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Virus infection elevates transcriptional activity of miR164a promoter in plants

Abstract
Background: Micro RNAs (miRs) constitute a large group of endogenous small RNAs that have crucial roles in many important plant functions. Virus infection and transgenic expression of viral proteins alter accumulation and activity of miRs and so far, most of the published evidence involves post-transcriptional regulations. Results: Using transgenic plants expressing a reporter gene under the promoter region of a characterized miR (P-miR164a), we [ver mas...]
Background: Micro RNAs (miRs) constitute a large group of endogenous small RNAs that have crucial roles in many important plant functions. Virus infection and transgenic expression of viral proteins alter accumulation and activity of miRs and so far, most of the published evidence involves post-transcriptional regulations. Results: Using transgenic plants expressing a reporter gene under the promoter region of a characterized miR (P-miR164a), we monitored the reporter gene expression in different tissues and during Arabidopsis development. Strong expression was detected in both vascular tissues and hydathodes. P-miR164a activity was developmentally regulated in plants with a maximum expression at stages 1.12 to 5.1 (according to Boyes, 2001) along the transition from vegetative to reproductive growth. Upon quantification of P-miR164a-derived GUS activity after Tobacco mosaic virus Cg or Oilseed rape mosaic virus (ORMV) infection and after hormone treatments, we demonstrated that ORMV and gibberellic acid elevated P-miR164a activity. Accordingly, total mature miR164, precursor of miR164a and CUC1 mRNA (a miR164 target) levels increased after virus infection and interestingly the most severe virus (ORMV) produced the strongest promoter induction. Conclusion: This work shows for the first time that the alteration of miR pathways produced by viral infections possesses a transcriptional component. In addition, the degree of miR alteration correlates with virus severity since a more severe virus produces a stronger P-miR164a induction. [Cerrar]
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Author
Bazzini, Ariel Alejandro;   Almasia, Natalia Ines;   Manacorda, Carlos Augusto;   Mongelli, Vanesa Claudia;   Conti, Gabriela;   Maroniche, Guillermo Andrés;   Rodriguez, Maria Cecilia;   Distefano, Ana Julia;   Hopp, Horacio Esteban;   Del Vas, Mariana;   Asurmendi, Sebastian;  
Fuente
BMC Plant Biology 9 : 152 (Diciembre 2009)
Date
2009-12
Editorial
BioMed Central
ISSN
1471-2229
URI
https://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-9-152
http://hdl.handle.net/20.500.12123/8665
DOI
https://doi.org/10.1186/1471-2229-9-152
Formato
pdf
Tipo de documento
artículo
Palabras Claves
Transgenic Plants; Plantas Transgénicas; Tobacco Mosaic Tobamovirus; Tobamovirus del Mosaico del Tabaco; Transcription; Transcripción;
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Excepto donde se diga explicitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
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