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Análisis de redes de coexpresión de la enfermedad cítrica Huanglongbing a partir de estudios de cinética de infección
Machado, Rodrigo; Moschen, Sergio; Gonzalez, Santiago Geronimo; Conti, Gabriela; Di Rienzo, Julio Alejandro; Burdyn, Lourdes; Hopp, Horacio Esteban; Fernandez, Paula Del Carmen (Universidad Nacional del Noroeste de la Provincia de Buenos Aires ( UNNOBA), 2022-10)HuangLongBing (HLB) es la enfermedad más devastadora de los cítricos y hasta el momento no cuenta con un tratamiento efectivo. Es causada por bacterias del género Candidatus Liberibacter spp. Estas bacterias son parásitos ... -
Bases moleculares de la interacción virus-planta : relación entre genes de defensa, ARNs pequeños y sintomatología
Conti, Gabriela (Facultad de Agronomía, Universidad de Buenos Aires, 2013-03)Los virus fitopatógenos producen alteraciones en el metabolismo y la fisiología de sus huéspedes provocadas principalmente por alteraciones en la expresión génica durante las infecciones. Numerosos cambios están asociados ... -
Citrus genetic transformation: an overview of the current strategies and insights on the new emerging technologies
Conti, Gabriela; Xoconostle-Cázares, Beatriz; Marcelino-Pérez, Gabriel; Hopp, Horacio Esteban; Reyes Martinez, Carina Andrea (Frontiers Media, 2021-11)Citrus are among the most prevailing fruit crops produced worldwide. The implementation of effective and reliable breeding programs is essential for coping with the increasing demands of satisfactory yield and quality of ... -
Differential mRNA accumulation upon early arabidopsis thaliana infection with ORMV and TMV-Cg Is associated with distinct endogenous small RNAs Level
Zavallo, Diego; Debat, Humberto Julio; Conti, Gabriela; Manacorda, Carlos Augusto; Rodriguez, Maria Cecilia; Asurmendi, Sebastian (2015-08-03)Small RNAs (sRNAs) play important roles in plant development and host-pathogen interactions. Several studies have highlighted the relationship between viral infections, endogenous sRNA accumulation and transcriptional ... -
Generación de una herramienta para la búsqueda de metadata asociada a transcriptos
Machado, Rodrigo; Moschen, Sebastian Nicolas; Gonzalez, Sergio Alberto; Conti, Gabriela; Massaro, Mora; Di Rienzo, Julio Alejandro; Burdyn, Lourdes; Hopp, Horacio Esteban; Fernandez, Paula Del Carmen (Sociedad Argentina de Informática (SADIO), 2021-10)Al analizar un transcriptoma en primera instancia se debe realizar un pre-procesamiento computacional de las lecturas de transcriptos generados por secuenciadores masivos y posterior obtención de la expresión diferencial ... -
Identificación y caracterización biológica y molecular de viroides en la región citrícola del Río Uruguay
Joris, Giovanna Lujan (Facultad de Ciencias Exactas, Universidad Nacional de La Plata, 2024-07)Los cítricos, al igual que otros cultivos, son sensibles a diversas enfermedades que impactan negativamente en la economía regional. Dentro de los patógenos que afectan a los cítricos se encuentran los viroides, patógenos ... -
Metabolic and miRNA profiling of TMV infected plants reveals biphasic temporal changes
Bazzini, Ariel Alejandro; Manacorda, Carlos Augusto; Tohge, Takayuki; Conti, Gabriela; Rodriguez, Maria Cecilia; Nunes Nesi, Adriano; Villanueva, Sofía; Fernie, Alisdair R.; Carrari, Fernando; Asurmendi, Sebastian (2011-12-12)Plant viral infections induce changes including gene expression and metabolic components. Identification of metabolites and microRNAs (miRNAs) differing in abundance along infection may provide a broad view of the pathways ... -
MicroRNA analysis of Taenia crassiceps cysticerci under praziquantel treatment and genome-wide identification of Taenia solium miRNAs
Pérez, Matías; Macchiaroli, Natalia; Lichtenstein, Gabriel; Conti, Gabriela; Asurmendi, Sebastian; Milone, Diego Humberto; Stegmayer, Georgina; Kamenetzky, Laura; Cucher, Marcela; Rosenzvit, Mara Cecilia (2017-09)MicroRNAs (miRNAs) are small non-coding RNAs that have emerged as important regulators of gene expression and perform critical functions in development and disease. In spite of the increased interest in miRNAs from helminth ... -
Modulation of host plant immunity by Tobamovirus proteins
Conti, Gabriela; Rodriguez, Maria Cecilia; Venturuzzi, Andrea Laura; Asurmendi, Sebastian (2017-03)To establish successful infection, plant viruses produce profound alterations of host physiology, disturbing unrelated endogenous processes and contributing to the development of disease. In tobamoviruses, emerging evidence ... -
Multiomics analyses reveal the roles of the ASR1 transcription factor in tomato fruits
Dominguez, Pia Guadalupe; Conti, Gabriela; Duffy, Tomas; Insani, Ester Marina; Alseekh, Saleh; Asurmendi, Sebastian; Fernie, Alisdair R.; Carrari, Fernando (Oxford University Press, 2021-09)The transcription factor ASR1 (ABA, STRESS, RIPENING 1) plays multiple roles in plant responses to abiotic stresses as well as being involved in the regulation of central metabolism in several plant species. However, despite ... -
Natural occurring epialleles determine vitamin E accumulation in tomato fruits
Quadrana, Leandro Daniel; Almeida de Souza, Juliana Beatriz; Asis, Ramón; Duffy, Tomas; Dominguez, Pia Guadalupe; Bermudez Salazar, Luisa; Conti, Gabriela; Silva, Junia V. Corrêa da; Peralta, Iris Edith; Colot, Vincent; Asurmendi, Sebastian; Fernie, Alisdair R.; Rossi, Magdalena; Carrari, Fernando Oscar (2014-07)Vitamin E (VTE) content is a low heritability nutritional trait for which the genetic determinants are poorly understood. Here, we focus on a previously detected major tomato VTE quantitative trait loci (QTL; mQTL9-2-6) ... -
Negative modulation of SA signaling components by the capsid protein of tobacco mosaic virus is required for viral long-distance movement
Venturuzzi, Andrea Laura; Rodriguez, Maria Cecilia; Conti, Gabriela; Leone, Melisa; Caro, Maria Del Pilar; Montecchia, Juan Francisco; Zavallo, Diego; Asurmendi, Sebastian (Wiley, 2021-05)An important aspect of plant–virus interaction is the way viruses dynamically move over long distances and how plant immunity modulates viral systemic movement. Salicylic acid (SA), a well-characterized hormone responsible ... -
Optimización de un diseño experimental para el estudio de la interacción Citrus - Candidatus Liberibacter spp., causante de la enfermedad HuangLongBing mediante análisis de RNA-seq
Machado, Rodrigo; Moschen, Sebastian Nicolas; Conti, Gabriela; Gonzalez, Sergio Alberto; Burdyn, Lourdes; Hopp, Horacio Esteban; Di Rienzo, Julio Alejandro; Fernandez, Paula Del Carmen (REDBIO, 2021-06)El método por excelencia actualmente utilizado para la detección de HLB es la PCR en tiempo real basado en la secuencia 16S rDNA. Sin embargo, la distribución irregular de las bacterias en las plantas, genera dificultades ... -
TMV induces RNA decay pathways to modulate gene silencing and disease symptoms
Conti, Gabriela; Zavallo, Diego; Venturuzzi, Andrea Laura; Rodriguez, Maria Cecilia; Crespi, Martin; Asurmendi, Sebastian (2017)RNA decay pathways comprise a combination of RNA degradation mechanisms that are implicated in gene expression, development and defense responses in eukaryotes. These mechanisms are known as the RNA Quality Control or RQC ... -
TMV-Cg Coat Protein stabilizes DELLA proteins and in turn negatively modulates salicylic acid-mediated defense pathway during Arabidopsis thaliana viral infection
Rodriguez, Maria Cecilia; Conti, Gabriela; Zavallo, Diego; Manacorda, Carlos Augusto; Asurmendi, Sebastian (2014)Background Plant viral infections disturb defense regulatory networks during tissue invasion. Emerging evidence demonstrates that a significant proportion of these alterations are mediated by hormone imbalances. Although ... -
Transgenic citrange troyer rootstocks overexpressing antimicrobial potato snakin-1 show reduced citrus canker disease symptoms
Conti, Gabriela; Gardella, Victoria; Vandecaveye, Melina Antonella; Gomez, Claudio Andres; Joris, Giovanna Lujan; Hauteville, Claudia; Burdyn, Lourdes; Almasia, Natalia Ines; Nahirñak, Vanesa; Vazquez Rovere, Cecilia; Gochez, Alberto Martin; Furman, Nicolas; Lezcano, Cecilia Carolina; Kobayashi, Ken; García, María Laura; Canteros, Blanca Isabel; Hopp, Horacio Esteban; Reyes, Carina Andrea (Elsevier, 2020-12)Citrus canker is a major disease caused by Xanthomonas citri pv. citri. Snakin-1 is an antimicrobial peptide, which was previously shown to be effective against different bacterial and fungal diseases in potato, wheat and ... -
Transgenic expression of tobacco mosaic virus capsid and movement proteins modulate plant basal defense and biotic stress responses in Nicotiana tabacum
Conti, Gabriela; Rodriguez, Maria Cecilia; Manacorda, Carlos Augusto; Asurmendi, Sebastian (2012)Plant viruses cause metabolic and physiological changes associated with symptomatic disease phenotypes. Symptoms involve direct and indirect effects, which result in disruption of host physiology. We used transgenic ... -
Virus infection elevates transcriptional activity of miR164a promoter in plants
Bazzini, Ariel Alejandro; Almasia, Natalia Ines; Manacorda, Carlos Augusto; Mongelli, Vanesa Claudia; Conti, Gabriela; Maroniche, Guillermo Andrés; Rodriguez, Maria Cecilia; Distefano, Ana Julia; Hopp, Horacio Esteban; Del Vas, Mariana; Asurmendi, Sebastian (BioMed Central, 2009-12)Background: Micro RNAs (miRs) constitute a large group of endogenous small RNAs that have crucial roles in many important plant functions. Virus infection and transgenic expression of viral proteins alter accumulation and ...