Instituto de Biotecnología
Browse by
Recent Submissions
-
Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding
(Pontificia Universidad Catolica de Valparaiso, 2010-09)Snakin-1 (StSN1) is a broad-spectrum antimicrobial peptide isolated from Solanum tuberosum. Homologous proteins have been identified in a wide range of species but there is no apparent consensus in the roles they play. A ... -
IL-12 DNA Displays efficient adjuvant effects improving immunogenicity of Ag85A in DNA prime/MVA boost immunizations
(Frontiers Media, 2020-09)Mycobacterium tuberculosis (Mtb) infection is one of the leading causes of death worldwide. The Modified Vaccinia Ankara (MVA) vaccine vector expressing the mycobacterial antigen 85A (MVA85A) was demonstrated to be safe, ... -
Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil
(Wiley, 2020-02)The aim of this study was to characterize Mycobacterium bovis from cattle and buffalo tissue samples, from two Brazilian states, and to analyse their genetic diversity by spoligotyping. Tissue samples from tuberculosis ... -
Transgenic citrange troyer rootstocks overexpressing antimicrobial potato snakin-1 show reduced citrus canker disease symptoms
(Elsevier, 2020-12)Citrus canker is a major disease caused by Xanthomonas citri pv. citri. Snakin-1 is an antimicrobial peptide, which was previously shown to be effective against different bacterial and fungal diseases in potato, wheat and ... -
Potato snakin‑1: an antimicrobial player of the trade‑off between host defense and development
(Springer, 2020-07)Snakin-1 (SN1) from potato is a cysteine-rich antimicrobial peptide with high evolutionary conservation. It has 63 amino acid residues, 12 of which are cysteines capable of forming six disulfide bonds. SN1 localizes in the ... -
Detection of Mycobacterium bovis–infected dairy herds using PCR in bulk tank milk samples
(Mary Ann Liebert Inc., 2012-02)Bovine tuberculosis (bTB) is a chronic and zoonotic disease due to Mycobacterium bovis. The tuberculosis eradication campaign carried out in Argentina has considerably improved the health situation of the herds. Here we ... -
Understanding the relationship between Mycobacterium bovis spoligotypes from cattle in Latin American Countries
(Elsevier, 2013-02)Spoligotyping is the most frequently used method for genotyping isolates of Mycobacterium bovis worldwide. In the current work, we compared spoligotypes from 1684 M. bovis isolates from Argentina (816), Brazil (412), Chile ... -
La genómica de las micobacterias
(Office International des Epizooties, 2016)Las especies Mycobacterium bovis y Mycobacterium avium subsp. paratuberculosis son los agentes causales de la tuberculosis y la paratuberculosis en animales, respectivamente. Además, ambas micobacterias, pero fundamentalmente ... -
LAMP technology: Rapid identification of Brucella and Mycobacterium avium subsp. paratuberculosis
(Sociedade Brasileira de Microbiologia, 2015-06)In this study, we developed new sets of primers to detect Brucella spp. and M. avium subsp. paratuberculosis (MAP) through isothermal amplification. We selected a previously well-characterized target gene, bscp31, specific ... -
Use of touch-down polymerase chain reaction to enhance the sensitivity of Mycobacterium bovis detection
(Sage Publications, 2005-05)The confirmatory diagnosis of Mycobacterium bovis (M. bovis) in animal samples is carried out by culture in Stonebrink media. However, culture is very slow because of the extremely long duplication time of the bacillus and ... -
Mycobacterium bovis infection in a horse with granulomatous enterocolitis
(Sage Publications, 2015-03)A 2-year-old dappled Percheron horse had a wasting condition that did not respond to antibiotic treatments and ultimately resulted in death. Thickening of the wall of the large colon and enlargement of the mesenteric lymph ... -
Mycobacterium intracellulare infection in a capybara (Hydrochoerus hydrochaeris)
(American Association of Zoo Veterinarians, 2013-12)This report describes the first case of Mycobacterium intracellulare infection with typical granulomatous lesions of mycobacteriosis in a capybara (Hydrochoerus hydrochaeris). The individual was a captive-bred young female, ... -
Patterns and processes of mycobacterium bovis evolution revealed by phylogenomic analyses
(Oxford University Press, 2017-03)Mycobacterium bovis is an important animal pathogen worldwide that parasitizes wild and domesticated vertebrate livestock as well as humans. A comparison of the five M. bovis complete genomes from the United Kingdom, South ... -
Draft genome sequence of Mycobacterium bovis 04-303, a highly virulent strain from Argentina
(American Society for Microbiology, 2013-11)Mycobacterium bovis strain 04-303 was isolated from a wild boar living in a free-ranging field in Argentina. This work reports the draft genome sequence of this highly virulent strain and the genomic comparison of its major ... -
Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts
(Elsevier, 2014-12)There has been an on-going debate on whether the development of drug resistance in Mycobacterium tuberculosis reduces its relative fitness and its ability to cause disease. The aim of this study was to explore this ... -
Pre-columbian mycobacterial genomes reveal seals as a source of new world human tuberculosis
(Springer Nature, 2014-08)Modern strains of Mycobacterium tuberculosis from the Americas are closely related to those from Europe, supporting the assumption that human tuberculosis was introduced post-contact1. This notion, however, is incompatible ... -
Mycobacterium bovis Dmce2 double deletion mutant protects cattle against challenge with virulent M. bovis
(Elsevier, 2013-05)A Mycobacterium bovis strain deleted in mce2A and mce2B genes (M. bovis Δmce2) was tested as an experimental vaccine in cattle challenged with a virulent M. bovis strain. Three-and-a-half-month old calves (n = 5 to 6 per ... -
Mycobacterioses in dogs and cats from Buenos Aires, Argentina
(Sage Publications, 2017-09)Mycobacterioses can produce nonspecific clinical signs in dogs and cats that make diagnosis difficult. Furthermore, the full characterization of mycobacterial agents is not always possible or practical. We characterized ... -
Bovine tuberculosis in domestic pigs: Genotyping and distribution of isolates in Argentina
(Elsevier, 2015-12)Bovine tuberculosis is caused by Mycobacterium bovis and affects primarily cattle, among many other mammal species. In this study, 250 isolates of M. bovis collected from pigs slaughtered in Argentina were typed by ... -
Tuberculosis in swine co-infected with Mycobacterium avium subsp. hominissuis and Mycobacterium bovis in a cluster from Argentina
(Cambridge University Press, 2015-04)In Argentina little is known about the epidemiology of tuberculosis (TB) infection in swine. We characterized the epidemiological dynamics of Mycobacterium avium complex (MAC) infection in a swine population of Argentina ...