Instituto de Biotecnología
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Exploring sunflower responses to Sclerotinia head rot at early stages of infection using RNA‑seq analysis
(Springer Nature, 2020-08)Sclerotinia head rot (SHR), caused by the necrotrophic fungus Sclerotinia sclerotiorum, is one of the most devastating sunflower crop diseases. Despite its worldwide occurrence, the genetic determinants of plant resistance ... -
The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far?
(Crimson Publishers, 2020-08)Specific mutations in the conserved domains of the acetolactate synthase (ALS) gene conduct to different key amino acid substitutions that can confer herbicide resistance in different plant species. This outcome has been ... -
Development and diagnostic validation of an ELISA based on an antigenic mixture for the detection of bovine tuberculosis
(Elsevier, 2020-02)Programs for the eradication of bovine tuberculosis (bTB) focus on the tuberculin skin test (TST) and slaughter of reactor cattle. However, the disease remains an animal health concern in several countries and improving ... -
Characterization of two GH5 endoglucanases from termite microbiome using synthetic metagenomics
(Springer, 2020-08)Here, we characterize two novel GH5 endoglucanases (GH5CelA and GH5CelB) from an uncultured bacterium identified in termite gut microbiomes. Both genes were codon-optimized, synthetized, cloned, and expressed as recombinant ... -
Development of diagnostic tools for IBDV detection using plants as bioreactors
(SpringerOpen, 2020-05)Infectious bursal disease virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds, thus causing important economic losses in the poultry industry. Multimeric ... -
Genomic re-assessment of the transposable element landscape of the potato genome
(Springer, 2020-05)Transposable elements (TEs) are DNA sequences with the ability to autoreplicate and move throughout the host genome. TEs are major drivers in stress response and genome evolution. Given their significance, the development ... -
Efficient method for targeted gene disruption by homologous recombination in Mycobacterium avium subspecie paratuberculosis
(Elsevier, 2020-09)Targeted gene disruption by homologous recombination, has been widely used in mycobacterium species to understand the genetic basis of virulence and persistence in the host and to develop efficacious potential live vaccines. ... -
Comparative analysis of the midgut microbiota of two natural tick vectors of Rickettsia rickettsii
(Elsevier, 2020-05)Although the ticks Amblyomma sculptum and Amblyomma aureolatum are important vectors of Rickettsia rickettsii, causative agent of the life-threatening Rocky Mountain spotted fever, A. aureolatum is considerably more ... -
Evaluation of hydrolytic enzyme activities from digestive fluids of Anthonomus grandis (Coleoptera: Curculionidae)
(Wiley, 2020-08)The cotton boll weevil, Anthonomus grandis, is a major pest of cotton crops in South America. In this work, partial biochemical characterizations of (hemi) cellulases and pectinases activities in the digestive system (head‐ ... -
Pathotype and SSR variation in Phytophthora sojae from the Argentinean Pampas
(Wiley, 2020-04)Genetic variation in Phytophthora sojae was compared between northern (NPS) and southern (SPS) Pampeana sub‐regions, in the core soya bean region of Argentina. Ninety‐three isolates from plants and soil were evaluated in ... -
Detección de bacterias de los géneros Ehrlichia, Anaplasma y Rickettsia en garrapatas Rhipicephalus sanguineus s.l en Pergamino, Argentina = Detection of Bacteria of the Genera Ehrlichia, Anaplasma and Rickettsia in Rhipicephalus sanguineus s.l Ticks in the City of Pergamino, Argentina
(Ministerio de Salud de la Nación, Argentina, 2019-12)INTRODUCCIÓN: Las rickettsiosis, ehrlichiosis y anaplasmosis son causadas por bacterias gramnegativas intracelulares obligadas y transmitidas por artrópodos. El objetivo de este trabajo fue determinar especies de garrapatas ... -
BoLA-DRB3 exon2 polymorphisms among tuberculous cattle: Nucleotide and functional variability and their association with bovine tuberculosis pathology
(Elsevier, 2020-06)Bovine tuberculosis (bTB) is caused by Mycobacterium bovis and disseminated worldwide. In Argentina, the highest prevalence occurs in dairy areas. BoLA DRB3.2 is related to the adaptive immunity in mycobacterial infections. ... -
Development of a copro-LAMP assay for detection of several species of Echinococcus granulosus sensu lato complex
(Elsevier, 2020-01)Cystic echinococcosis represents a significant problem in human and animal health and constitutes one of the most severe Neglected Tropical Diseases prioritized by the World Health Organization. The etiological agent is ... -
Spontaneous Mutations in the Nitrate Reductase Gene napC Drive the Emergence of Eco-friendly Low-N2O-Emitting Alfalfa Rhizobia in Regions with Different Climates
(Springer, 2020)We have recently shown that commercial alfalfa inoculants (e.g., Sinorhizobium meliloti B399), which are closely related to the denitrifier model strain Sinorhizobium meliloti 1021, have conserved nitrate, nitrite, and ... -
Desarrollo y evaluación de un candidato vacunal para IBD producido en plantas: una alternativa a las vacunas tradicionales
(Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 2018)El Virus de la enfermedad infecciosa de la bursa (IBDV) es el agente etiológico responsable de una enfermedad aguda contagiosa e inmunosupresora que afecta pollos jóvenes y causa importantes pérdidas económicas. La vacunación ... -
Análisis del microbioma de insectos: identificación y caracterización de glicosil hidrolasas
(Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 2018)Algunos insectos han desarrollado estrategias muy eficaces para utilizar sustratos lignocelulósicos como fuentes de energía. En termitas y algunos coleópteros, la digestión de la lignocelulosa es el resultado de la acción ... -
Unveiling the genetic basis of Sclerotinia head rot resistance in sunflower
(BMC, 2020-07)Background: Sclerotinia sclerotiorum is a necrotrophic fungus that causes Sclerotinia head rot (SHR) in sunflower, with epidemics leading to severe yield losses. In this work, we present an association mapping (AM) approach ... -
Tuberculosis bovina : transmisibilidad de cepas de Mycobacterium bovis y detección de micobacterias en menudencias comercializadas en bocas de expendio de carne
(Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, 2019)La tuberculosis bovina, causada por Mycobacterium bovis (M. bovis), es endémica en la Argentina. Su presencia en el ganado bovino representa un riesgo para la salud humana debido a su carácter zoonótico, pudiendo además ... -
Prospección y caracterización de bacterias celulolíticas y hemicelulolíticas
(Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 2018)La prospección de bacterias con capacidad de degradar lignocelulosa permite la identificación de nuevas enzimas con potencial aplicación en numerosos procesos industriales. En este trabajo el objetivo fue la obtención de ... -
A candidate gene survey of quantitative trait loci affecting chemical composition in tomato fruit
(Society for Experimental Biology, 2008-07)In tomato, numerous wild-related species have been demonstrated to be untapped sources of valuable genetic variability, including pathogen-resistance genes, nutritional, and industrial quality traits. From a collection of ...