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AcMNPV Core Gene ac109 Is Required for Budded Virion Transport to the Nucleus and for Occlusion of Viral Progeny
Alfonso, Victoria; Maroniche, Guillermo Andrés; Reca, Sol Rita; Lopez, Maria Gabriela; Del Vas, Mariana; Taboga, Oscar Alberto (Plos One, 2012-09)The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac109 core gene has been previously characterized as an essential late gene. Our results showed that budded virions could be detected in supernatants of ... -
Development of a novel set of Gateway-compatible vectors for live imaging in insect cells
Maroniche, Guillermo Andrés; Mongelli, Vanesa Claudia; Alfonso, Victoria; Llauger, Gabriela; Taboga, Oscar Alberto; Del Vas, Mariana (The Royal Entomological Society, 2011-10)Insect genomics is a growing area of research. To exploit fully the genomic data that are being generated, high-throughput systems for the functional characterization of insect proteins and their interactomes are required. ... -
Estudio de Azospirillum como tecnología aplicable en los cultivos de trigo y maíz = Study of Azospirillum as an applicable technology in wheat and corn crops
Garcia, Julia Elena; Puente, Mariana Laura; Maroniche, Guillermo Andrés; Perticari, Alejandro (Magna Publicaciones, 2013-08)Los efectos de la inoculación con bacterias promotoras en cereales son ampliamente estudiados en todo el mundo e incluso también en Argentina, aunque es limitado el número de ensayos a campo utilizando diferentes cepas y ... -
Estudio de la interacción hospedante-patógeno entre el Mal de Río Cuarto virus (MRCV) y el delfácido transmisor Delphacodes kuscheli
Maroniche, Guillermo Andrés (Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 2011)El virus del Mal de Río Cuarto (MRCV, Reoviridae, Fijivirus) causa la principal enfermedad que afecta al maíz en la Argentina. Su genoma consiste en diez segmentos de ARN de doble cadena (S1 a S10) que codifican para un ... -
Functional and biochemical properties of Mal de Río Cuarto virus (Fijivirus, Reoviridae) P9-1 viroplasm protein show further similarities to animal reovirus counterparts
Maroniche, Guillermo Andrés; Mongelli, Vanesa Claudia; Peralta, Andrea Veronica; Distefano, Ana Julia; Llauger, Gabriela; Taboga, Oscar Alberto; Hopp, Horacio Esteban; Del Vas, Mariana (Elsevier, 2010-09)Mal de Río Cuarto virus (MRCV) is a plant virus of the genus Fijivirus within the family Reoviridae that infects several monocotyledonous species and is transmitted by planthoppers in a persistent and propagative manner. ... -
Genome-based reclassification of Azospirillum brasilense Az39 as the type strain of Azospirillum argentinense sp. nov.
dos Santos Ferreira, Natália; Coniglio, Nayla Anahí; Puente, Mariana Laura; Sant’Anna, Fernando Hayashi; Maroniche, Guillermo Andrés; Garcia, Julia Elena; Molina, Romina Micaela; Nievas, Sofía; Volpiano, Camila Gazolla; Ambrosini, Adriana; Passaglia, Luciane Maria Pereira; Pedraza, Raúl Osvaldo; Reis, Veronica Massena; Zilli, Jerri Edson; Cassan, Fabricio Dario (Microbiology Society, 2022-08-01)Strain Az39T of Azospirillum is a diazotrophic plant growth-promoting bacterium isolated in 1982 from the roots of wheat plants growing in Marcos Juárez, Córdoba, Argentina. It produces indole-3- acetic acid in the presence ... -
In vitro PGPR properties and osmotic tolerance of different Azospirillum native strains and their effects on growth of maize under drought stress
Garcia, Julia Elena; Maroniche, Guillermo Andrés; Creus, Cecilia; Suárez-Rodríguez, Ramón; Ramirez-Trujillo, José Augusto; Groppa, María D. (2017-09)Osmotic variations in the soil can affect bacterial growth diminishing the number of inoculated bacteria. In a scenario of water deficit having tolerant bacteria would be beneficial to achieve a better response of the plant ... -
In vivo subcellular localization of Mal de Río Cuarto virus (MRCV) non-structural proteins in insect cells reveals their putative functions
Maroniche, Guillermo Andrés; Mongelli, Vanesa Claudia; Llauger, Gabriela; Alfonso, Victoria; Taboga, Oscar Alberto; Del Vas, Mariana (Elsevier, 2012-09)The in vivo subcellular localization of Mal de Río Cuarto virus (MRCV, Fijivirus, Reoviridae) non-structural proteins fused to GFP was analyzed by confocal microscopy. P5-1 showed a cytoplasmic vesicular-like distribution ... -
Inoculation with Azospirillum argentinense Az19 improves the yield of maize subjected to water deficit at key stages of plant development = La inoculación con Azospirillum argentinense Az19 mejora el rendimiento del maíz expuesto a déficit hídrico durante estadios clave del desarrollo vegetal
Garcia, Julia Elena; Ruiz, Monica Beatriz; Maroniche, Guillermo Andrés; Creus, Cecilia; Puente, Mariana Laura; Zawoznik, Myriam Sara; Groppa, María Daniela (Elsevier, 2023-07)Water deficit constitutes a severe limitation to agricultural productivity. In the context of sustainable crop production, the potential of microbial biotechnology to increase plant drought tolerance and improve crop yields ... -
Localization and survival of Azospirillum brasilense Az39 in soybean leaves
Puente, Mariana Laura; Maroniche, Guillermo Andrés; Panepucci, Marina Gabriela; Sabio Y Garcia, Julia Veronica; García, Julia Elena; Criado, María Victoria; Molina, Romina Micaela; Cassán, Fabricio Dario (Wiley, 2021-05)In recent years, foliar inoculation has gained acceptance among the available methods to deliver plant beneficial micro-organisms to crops under field conditions. Colonization efficiency by such micro-organisms largely ... -
Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation
Garcia, Julia Elena; Pagnussat, Luciana Anabella; Amenta, Melina B.; Casanovas, E. Mabel; Diaz, Pablo R.; Labarthe, María Mercedes; Martino, María V.; Groppa, María Daniela; Creus, Cecilia Mónica; Maroniche, Guillermo Andrés (Springer, 2024-12-27)Azospirillum argentinense Az19 is an osmotolerant plant growth-promoting bacterium that protects maize plants from drought. In this work, we explored the role of trehalose in the superior performance of Az19 under stress. ... -
Microbial biostimulants to mitigate salt stress in lettuce
Moya, Karen; Maroniche, Guillermo Andrés; Vallejo, Daniela Adriana; Puente, Mariana Laura; Di Salvo, Luciana; Garcia, Julia Elena (Sociedad Argentina de Microbiología General (SAMIGE), 2024-10-22)Plant growth-promoting rhizobacteria (PGPR) can stimulate plant growth, increase yield, reduce pathogen infection, as well as reduce abiotic plant stress. These microorganisms are of interest for application in agriculture. ... -
Molecular identification of Azospirillum spp.: limitations of 16S rRNA and qualities of rpoD as genetic markers
Maroniche, Guillermo Andrés; Garcia, Julia Elena; Salcedo, Florencia; Creus, Cecilia Mónica (2017)Since their discovery, plant-growth promoting rhizobacteria from the genus Azospirillum have been sub-jected to intensive research due to their biotechnological potential as crop inoculants. Phylogeneticanalysis of ... -
Phenogenetic profile and agronomic contribution of Azospirillum argentinense Az39T, a reference strain for the South American inoculant industry
Maroniche, Guillermo Andrés; Puente, Mariana Laura; Garcia, Julia Elena; Mongiardini, Elias Javier; Coniglio, Nayla Anahí; Nievas, Sofía Mariela; Labarthe, María Mercedes; Wisniewski-Dyé, Florence; Rodriguez Cáceres, Enrique; Diaz Zorita, Martin; Cassán, Fabricio Darío (Elsevier, 2024-02-15)Azospirillum sp. is a plant growth-promoting rhizobacteria largely recognized for its potential to increase the yield of different important crops. In this work, we present a thorough genomic and phenotypic analysis of A. ... -
Potential of rhizobacteria native to Argentina for the control of Meloidogyne javanica.
Borrajo, Paula; Mondino, Eduardo Ariel; Maroniche, Guillermo Andrés; Fernández, Macarena; Creus, Cecilia (Asociación Argentina de Microbiología, 2021-05-02)Biocontrol of the nematode Meloidogyne javanica was studied using the Argentinean strains Pseudomonas fluorescens MME3, TAE4, TAR5 and ZME4 and Bacillus sp. B7S, B9T and B19S. Pseudomonas protegens CHA0 was used as a ... -
Reference gene selection for gene expression studies using RT-qPCR in virus-infected planthoppers
Maroniche, Guillermo Andrés; Sagadin, Monica Beatriz; Mongelli, Vanesa Claudia; Truol, Graciela Ana Maria; Del Vas, Mariana (BioMed Central, 2011-12)Background: Planthoppers not only severely affect crops by causing mechanical damage when feeding but are also vectors of several plant virus species. The analysis of gene expression in persistently infected planthoppers ... -
Signs of a phyllospheric lifestyle in the genome of the stress-tolerant strain Azospirillum brasilense Az19
Garcia, Julia Elena; Labarthe, María Mercedes; Pagnussat, Luciana Anabella; Amenta, Melina Beatriz; Creus, Cecilia Mónica; Maroniche, Guillermo Andrés (Elsevier, 2020-11)Azospirillum brasilense Az19 is a plant-beneficial bacterium capable of protecting plants from the negative effects of drought. The objective of this study was to determine and analyze the genomic sequence of strain Az19 ... -
Successful production of the potato antimicrobial peptide Snakin-1 in baculovirus-infected insect cells and development of specific antibodies
Almasia, Natalia Ines; Molinari, Maria Paula; Maroniche, Guillermo Andrés; Nahirñak, Vanesa; Barrios Baron, Maria Pilar; Taboga, Oscar Alberto; Vazquez Rovere, Cecilia (2017)Background: Snakin-1 (StSN1) is a broad-spectrum antimicrobial cysteine-rich peptide isolated from Solanum tuberosum. Its biotechnological potential has been already recognized since it exhibits in vivo antifungal and ... -
Virus infection elevates transcriptional activity of miR164a promoter in plants
Bazzini, Ariel Alejandro; Almasia, Natalia Ines; Manacorda, Carlos Augusto; Mongelli, Vanesa Claudia; Conti, Gabriela; Maroniche, Guillermo Andrés; Rodriguez, Maria Cecilia; Distefano, Ana Julia; Hopp, Horacio Esteban; Del Vas, Mariana; Asurmendi, Sebastian (BioMed Central, 2009-12)Background: Micro RNAs (miRs) constitute a large group of endogenous small RNAs that have crucial roles in many important plant functions. Virus infection and transgenic expression of viral proteins alter accumulation and ...