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Variability in spectrophotometric pyruvate analyses for predicting onion pungency and nutraceutical value

Abstract
Onion pyruvate concentration is used as a predictor of flavor intensity and nutraceutical value. The protocol of Schwimmer and Weston (SW) (1961) is the most widespread methodology for estimating onion pyruvate. Anthon and Barret (AB) (2003) proposed modifications to this procedure. Here, we compared these spectrophotometry-based procedures for pyruvate analysis using a diverse collection of onion cultivars. The SW method always led to over-estimation [ver mas...]
Onion pyruvate concentration is used as a predictor of flavor intensity and nutraceutical value. The protocol of Schwimmer and Weston (SW) (1961) is the most widespread methodology for estimating onion pyruvate. Anthon and Barret (AB) (2003) proposed modifications to this procedure. Here, we compared these spectrophotometry-based procedures for pyruvate analysis using a diverse collection of onion cultivars. The SW method always led to over-estimation of pyruvate levels in colored, but not in white onions, by up to 65%. Identification of light-absorbance interfering compounds was performed by spectrophotometry and HPLC analysis. Interference by quercetin and anthocyanins, jointly, accounted for more than 90% of the over-estimation of pyruvate. Pyruvate determinations according to AB significantly reduced absorbance interference from compounds other than pyruvate. This study provides evidence about the mechanistic basis underlying differences between the SW and AB methods for indirect assessment of onion flavor and nutraceutical value. [Cerrar]
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Author
Beretta, Hebe Vanesa;   Bannoud, Florencia;   Insani, Ester Marina;   Galmarini, Claudio Romulo;   Cavagnaro, Pablo;  
Fuente
Food chemistry 224 : 201-206. (June 2017)
Date
2017-06
ISSN
0308-8146
URI
http://hdl.handle.net/20.500.12123/1349
http://www.sciencedirect.com/science/article/pii/S0308814616320404
DOI
https://doi.org/10.1016/j.foodchem.2016.12.031
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article
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Excepto donde se diga explicitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
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