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Storage lipids and proteins of Euterpe edulis seeds
Abstract
Comparative studies on fatty acid and protein composition of the endosperm and embryo of palmito (Euterpe edulis Martius) were conducted using gas-liquid chromatography and sodium dodecyl sulfate– polyacrylamide gel electrophoresis. On a dry weight basis, the embryo contained extremely lower amounts of lipids and proteins than did the endosperm, which was associated with the scarce lipid and protein bodies previously reported in axis and cotyledon. The
[ver mas...]
Comparative studies on fatty acid and protein composition of the endosperm and embryo of palmito (Euterpe edulis Martius) were conducted using gas-liquid chromatography and sodium dodecyl sulfate– polyacrylamide gel electrophoresis. On a dry weight basis, the embryo contained extremely lower amounts of lipids and proteins than did the endosperm, which was associated with the scarce lipid and protein bodies previously reported in axis and cotyledon. The fatty acid composition also exhibited differences between
both tissues: (I) the fatty acid diversity was greater in embryo than in endosperm; (II) embryo and endosperm contained predominantly linoleic, palmitic, oleic and stearic acids even though the relative values were different for each tissue. As compared to other palm species, the higher fatty acid unsaturation in Euterpe edulis seed could be involved in the previously reported short longevity and recalcitrant behavior during storage.
Proteins of both tissues were heterogeneous in molecular mass. Some proteins were tissue-specific, but other were common, among them a highly glycosylated protein which migrated at about 55 kDa. We hypothesize that the latter, also reported in all previously studied palm species, is one of the proteins characterizing the Arecaceae family.
[Cerrar]
Estudios comparativos sobre la composición de ácidos grasos y proteínas del endospermo y el embrión de palmito (Euterpe edulis Martius) mediante cromatografía gas-líquido y dodecilsulfato de sodio– Electroforesis en gel de poliacrilamida. Sobre una base de peso seco, el embrión contenía cantidades extremadamente bajas de lípidos y proteínas que el endospermo, que estaba asociado con los escasos cuerpos de lípidos y proteínas reportado previamente en eje y
[ver mas...]
Estudios comparativos sobre la composición de ácidos grasos y proteínas del endospermo y el embrión de palmito (Euterpe edulis Martius) mediante cromatografía gas-líquido y dodecilsulfato de sodio– Electroforesis en gel de poliacrilamida. Sobre una base de peso seco, el embrión contenía cantidades extremadamente bajas de lípidos y proteínas que el endospermo, que estaba asociado con los escasos cuerpos de lípidos y proteínas reportado previamente en eje y cotiledón. La composición de ácidos grasos también mostró diferencias entre ambos tejidos: (I) la diversidad de ácidos grasos fue mayor en el embrión que en el endospermo; (II) embrión y endospermo contenía predominantemente ácidos linoleico, palmítico, oleico y esteárico, aunque los valores relativos eran diferentes para cada tejido. En comparación con otras especies de palmeras, la mayor insaturación de ácidos grasos en Euterpe edulis la semilla podría estar involucrada en la corta longevidad y el comportamiento recalcitrante durante el almacenamiento informados anteriormente. Las proteínas de ambos tejidos eran heterogéneas en masa molecular. Algunas proteínas eran específicas de tejido, pero otras eran comunes, entre ellos una proteína altamente glicosilada que migraba a aproximadamente 55 kDa. Nosotros hipotetizamos
que esta última, también reportada en todas las especies de palmeras previamente estudiadas, es una de las proteínas que caracterizan la familia Arecaceae.
[Cerrar]

Author
Panza, Victor;
Pighin, Darío Gabriel;
Láinez, Verónica;
Pollero, Ricardo José;
Maldonado, Sara;
Fuente
Biocell (Mendoza) 33 (2) : 99-106. (2009).
Date
2009
Editorial
Sociedad Latinoamericana de Microscopía Electrónica y Centro Regional de Investigaciones Científicas y Tecnológicas (Mendoza, Argentina)
ISSN
0327-9545
Formato
pdf
Tipo de documento
artículo
Palabras Claves
Derechos de acceso
Abierto

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