View Item
- xmlui.general.dspace_homeCentros e Institutos de InvestigaciónCICVyA. Centro de Investigación en Ciencias Veterinarias y AgronómicasInstituto de BiotecnologíaArtículos científicosxmlui.ArtifactBrowser.ItemViewer.trail
- DSpace Home
- Centros e Institutos de Investigación
- CICVyA. Centro de Investigación en Ciencias Veterinarias y Agronómicas
- Instituto de Biotecnología
- Artículos científicos
- View Item
Development of a lateral flow immunochromatography test for the rapid detection of bovine tuberculosis
Abstract
Detection of specific antibodies would be a useful test strategy for bovine tuberculosis (bTB) as a complement to the single skin test. We developed a lateral flow immunochromatography (LFIC) test for rapid bTB detection based on the use of a conjugate of gold nanoparticles with a recombinant G protein. After evaluating 3 Mycobacterium bovis (MB) antigens: ESAT-6, CFP-10 and MPB83 for the control line, we selected MPB83 given it was the most specific. The
[ver mas...]
Detection of specific antibodies would be a useful test strategy for bovine tuberculosis (bTB) as a complement to the single skin test. We developed a lateral flow immunochromatography (LFIC) test for rapid bTB detection based on the use of a conjugate of gold nanoparticles with a recombinant G protein. After evaluating 3 Mycobacterium bovis (MB) antigens: ESAT-6, CFP-10 and MPB83 for the control line, we selected MPB83 given it was the most specific. The performance of the test was analyzed with 820 bovine sera, 40 sera corresponding to healthy animals, 5 sera from animals infected with Mycobacterium avium subsp. paratuberculosis (MAP) and 775 sera of animals from herds with bTB. All these sera were also submitted to a validated bTB-ELISA using whole-cell antigen from MB. From the 775 sera of animals from herds with bTB, 87 sera were positive by the bTB-ELISA, 45 were positive by LFIC and only 5 animals were positives by skin test (TST). To confirm bTB infection in the group of TST (−), bTB-ELISA (+) and LFIC (+) animals, we performed postmortem examination in 15 randomly selected animals. Macroscopically, these 15 animals had numerous small and large yellow-white granulomas, characteristic of bTB, and the infection was subsequently confirmed by PCR in these tissues with lesions (gold standard).
No false positive test result was detected with the developed LFIC either with the sera from healthy animals or from animals infected with MAP demonstrating that it can be a useful technique for the rapid identification of animals infected with bTB.
[Cerrar]
![Thumbnail](/bitstream/handle/20.500.12123/9147/Journal%20of%20Immunological%20Methods_cover.gif?sequence=4&isAllowed=y)
Author
Alonso, Maria Natalia;
Griffa, Natanael;
Moyano, Roberto Damián;
Mon, Maria Laura;
Colombatti Olivieri, Maria Alejandra;
Barandiaran, Soledad;
Martinez Vivot, Marcela;
Fiorini, Gonzalo;
Canal, Ana María;
Santangelo, María De La Paz;
Singh, Mahavir G.;
Romano, Maria Isabel;
Fuente
Journal of Immunological Methods 491 : 112941 (Abril 2021)
Date
2021-04
Editorial
Elsevier
ISSN
1872-7905
Formato
pdf
Tipo de documento
artículo
Palabras Claves
Derechos de acceso
Restringido
![](../../themes/Mirage2/images/cc-by-nc-sa.png)
Metadata
Show full item recordRelated items
Showing items related by title, author, creator and subject.
-
Evaluation of a virulent strain of Mycobacterium avium subsp. Paratuberculosis used as a heat-killed vaccine
Colombatti Olivieri, Maria Alejandra; Moyano, Roberto Damian; Mon, Maria Laura; Gravisaco, María José; Alvarado Pinedo, María Fiorella; Delgado, Fernando Oscar; Hernández Pando, Rogelio; Alonso, Maria Natalia; Cuerda, Maria Ximena; Santangelo, María De La Paz; Romano, Maria Isabel (Elsevier, 2021-11)Bovine paratuberculosis is one of the most important chronic infectious diseases in livestock. This disease is difficult to control because of its inefficient management (test and cull strategy and inadequate biosecurity). ... -
Buenas prácticas lecheras : guía para la implementación en la producción de leche bovina
Aimar, María Verónica; Amalfi, José María; Campos Carlés, Silvina; Charlon, Veronica; Cristiano, Gabriela; Delbino, Fernando; Diaz Cano, Muncha; Estevez Acuña, Rodrigo Rubén; Gigli, Isabel; Glauber, Claudio Eduardo; Guillermón, José; Ibargoyen, José María; Iorio, Jesica Daniela; Jaureguiberry, Horacio Sebastián; Jarkowsky, Andrés; Lesman, María de los Ángeles; Llano, Agustina; Mazzitelli, José; Moro, Santiago; Mozeris, Gustavo; Negri Rodriguez, Livia Maria; Olivieri, Daniela Ivana; Osan, Oscar; Pérez, Gabriela Guadalupe; Preumayr, Fernando Martín; Serrano, Pedro Miguel; Tonero, María Eugenia; Trombert, José María; Troya, Paula; Urruspuru, María Luz; Varaldo, Estefanía (Red de Buenas Prácticas Agropecuarias - BPA, 2021-03)Las “Buenas Prácticas Agropecuarias” hacen referencia a una producción sana, segura y amigable con el ambiente, los animales y las personas. Son numerosos los temas que abarca e involucra la producción primaria de leche: ... -
Zuccagnia‐type Propolis from Argentina: A potential functional ingredient in food to pathologies associated to metabolic syndrome and oxidative stress
Salas, Ana Lilia; Mercado, María Inés; Orqueda, Maria Eugenia; Correa Uriburu, Florencia; García, Maria Elena; Pérez, María Jorgelina; Alvarez, María de los Angeles; Ponessa, Graciela I.; Maldonado, Luis Maria; Zampini, Iris Catiana; Isla, María Inés (Wiley, 2020-07)The effect of Argentine propolis extracts against enzymes related to metabolic syndrome and oxidative stress, as well as the botanical origin of raw propolis, were studied. Histological and chemical analyses of propolis ...