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Development and diagnostic validation of an ELISA based on an antigenic mixture for the detection of bovine tuberculosis
Griffa, Natanael; Moyano, Roberto Damián; Canal, Ana María; Traveria, Gabriel Eduardo; Santangelo, María De La Paz; Alonso, Maria Natalia; Romano, Maria Isabel (Elsevier, 2020-02)Programs for the eradication of bovine tuberculosis (bTB) focus on the tuberculin skin test (TST) and slaughter of reactor cattle. However, the disease remains an animal health concern in several countries and improving ... -
Development of a lateral flow immunochromatography test for the rapid detection of bovine tuberculosis
Alonso, Maria Natalia; Griffa, Natanael; Moyano, Roberto Damián; Mon, Maria Laura; Colombatti Olivieri, Maria Alejandra; Barandiaran, Soledad; Martinez Vivot, Marcela; Fiorini, Gonzalo; Canal, Ana María; Santangelo, María De La Paz; Singh, Mahavir G.; Romano, Maria Isabel (Elsevier, 2021-04)Detection of specific antibodies would be a useful test strategy for bovine tuberculosis (bTB) as a complement to the single skin test. We developed a lateral flow immunochromatography (LFIC) test for rapid bTB detection ... -
Efficient method for targeted gene disruption by homologous recombination in Mycobacterium avium subspecie paratuberculosis
Alonso, Maria Natalia; Malaga, Wladimir; Mc Neil, Michael; Jackson, Mary; Romano, Maria Isabel; Guilhot, Christophe; Santangelo, María De La Paz (Elsevier, 2020-09)Targeted gene disruption by homologous recombination, has been widely used in mycobacterium species to understand the genetic basis of virulence and persistence in the host and to develop efficacious potential live vaccines. ... -
Evaluation of a virulent strain of Mycobacterium avium subsp. Paratuberculosis used as a heat-killed vaccine
Colombatti Olivieri, Maria Alejandra; Moyano, Roberto Damian; Mon, Maria Laura; Gravisaco, María José; Alvarado Pinedo, María Fiorella; Delgado, Fernando Oscar; Hernández Pando, Rogelio; Alonso, Maria Natalia; Cuerda, Maria Ximena; Santangelo, María De La Paz; Romano, Maria Isabel (Elsevier, 2021-11)Bovine paratuberculosis is one of the most important chronic infectious diseases in livestock. This disease is difficult to control because of its inefficient management (test and cull strategy and inadequate biosecurity). ...