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Propidium monoazide (PMA) is a selective nucleic acid intercalating dye that can be combined with real‐time PCR (qPCR) in order to evaluate cell viability in food samples. The aim of the present work was to evaluate PMA‐qPCR to detect and quantify viable STEC cells in beef burgers using stx2 as target gene. First, it was determined that 100 µM of PMA could inhibit qPCR signal from non‐viable cells and had no influence on the amplification of different
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dc.contributor.author | Rey, Maria De Los Angeles | |
dc.contributor.author | Cap, Mariana | |
dc.contributor.author | Favre, Leonardo Cristian | |
dc.contributor.author | Rodriguez, Anabel | |
dc.contributor.author | Dus Santos, Maria Jose | |
dc.contributor.author | Vaudagna, Sergio Ramon | |
dc.contributor.author | Mozgovoj, Marina Valeria | |
dc.date | info:eu-repo/date/embargoEnd/2022-03-12 | |
dc.date.accessioned | 2021-03-15T12:10:09Z | |
dc.date.available | 2021-03-15T12:10:09Z | |
dc.date.issued | 2021-02-06 | |
dc.identifier.issn | 1745-4549 | |
dc.identifier.other | https://doi.org/10.1111/jfpp.15338 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/8891 | |
dc.identifier.uri | https://ifst.onlinelibrary.wiley.com/doi/10.1111/jfpp.15338 | |
dc.description.abstract | Propidium monoazide (PMA) is a selective nucleic acid intercalating dye that can be combined with real‐time PCR (qPCR) in order to evaluate cell viability in food samples. The aim of the present work was to evaluate PMA‐qPCR to detect and quantify viable STEC cells in beef burgers using stx2 as target gene. First, it was determined that 100 µM of PMA could inhibit qPCR signal from non‐viable cells and had no influence on the amplification of different concentrations of viable cells. Then, it was shown that PMA efficiently distinguished between different log cfu of viable cells in presence of a high concentration of non‐viable cells, both in culture and in beef burger homogenates. Finally, it was determined that PMA could distinguish between viable and non‐viable cells within the same log cfu in beef burger homogenates. PMA‐qPCR effectively detected and quantified viable STEC cells in culture and in beef burger homogenates. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.relation | info:eu-repograntAgreement/INTA/2019-PE-E7-I147-001/2019-PE-E7-I147-001/AR./INOCUIDAD DE ALIMENTOS PARA CONSUMO HUMANO Y ANIMAL | es_AR |
dc.relation | info:eu-repograntAgreement/INTA/PNAIyAV-1130042/AR./Herramientas y sistemas para la gestión de la calidad integral. | |
dc.rights | info:eu-repo/semantics/embargoedAccess | es_AR |
dc.source | Journal Food Processing and Preservation 45 (3) : e15338 (2021) | es_AR |
dc.subject | Escherichia coli | es_AR |
dc.subject | Viability | eng |
dc.subject | Minced Meat | eng |
dc.subject | Carne Picada | es_AR |
dc.subject.other | Beef Burgers | eng |
dc.subject.other | Hamburguesas de Ternera | es_AR |
dc.subject.other | Propidium monoazide | eng |
dc.subject.other | Monoazida de propidio | es_AR |
dc.subject.other | qPCR | es_AR |
dc.subject.other | PCR en tiempo real (qPCR) | es_AR |
dc.title | Evaluation of PMA‐qPCR methodology to detect and quantify viable Shiga toxin‐producing Escherichia coli in beef burgers | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/acceptedVersion | es_AR |
dc.description.origen | Instituto de Investigación de Tecnología de Alimentos | es_AR |
dc.description.fil | Fil: Rey, María De Los Ángeles. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina. | es_AR |
dc.description.fil | Fil: Rey, María De Los Ángeles. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables (UEDD INTA CONICET); Argentina. | es_AR |
dc.description.fil | Fil: Cap, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina. | es_AR |
dc.description.fil | Fil: Cap, Mariana. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables (UEDD INTA CONICET); Argentina. | es_AR |
dc.description.fil | Fil: Favre, Leonardo Cristian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina. | es_AR |
dc.description.fil | Fil: Favre, Leonardo Cristian. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables (UEDD INTA CONICET); Argentina. | es_AR |
dc.description.fil | Fil: Rodriguez, Anabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina. | es_AR |
dc.description.fil | Fil: Rodriguez, Anabel. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables (UEDD INTA CONICET); Argentina. | es_AR |
dc.description.fil | Fil: Dus Santos, Maria Jose. Instituto de Virología e Innovaciones tecnológicas. Centro de Investigaciones en Ciencias Veterinarias y Agronómicas (INTA-CONICET); Argentina. | es_AR |
dc.description.fil | Fil: Vaudagna Sergio Ramon. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina. | es_AR |
dc.description.fil | Fil: Vaudagna Sergio Ramon. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables (UEDD INTA CONICET); Argentina. | es_AR |
dc.description.fil | Fil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina. | es_AR |
dc.description.fil | Fil: Mozgovoj, Marina Valeria. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables (UEDD INTA CONICET); Argentina. | es_AR |
dc.subtype | cientifico |
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