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Abstract
Propidium monoazide (PMA) is a selective nucleic acid intercalating dye that can be combined with real‐time PCR (qPCR) in order to evaluate cell viability in food samples. The aim of the present work was to evaluate PMA‐qPCR to detect and quantify viable STEC cells in beef burgers using stx2 as target gene. First, it was determined that 100 µM of PMA could inhibit qPCR signal from non‐viable cells and had no influence on the amplification of different [ver mas...]
dc.contributor.authorRey, Maria De Los Angeles
dc.contributor.authorCap, Mariana
dc.contributor.authorFavre, Leonardo Cristian
dc.contributor.authorRodriguez, Anabel
dc.contributor.authorDus Santos, Maria Jose
dc.contributor.authorVaudagna, Sergio Ramon
dc.contributor.authorMozgovoj, Marina Valeria
dc.dateinfo:eu-repo/date/embargoEnd/2022-03-12
dc.date.accessioned2021-03-15T12:10:09Z
dc.date.available2021-03-15T12:10:09Z
dc.date.issued2021-02-06
dc.identifier.issn1745-4549
dc.identifier.otherhttps://doi.org/10.1111/jfpp.15338
dc.identifier.urihttp://hdl.handle.net/20.500.12123/8891
dc.identifier.urihttps://ifst.onlinelibrary.wiley.com/doi/10.1111/jfpp.15338
dc.description.abstractPropidium monoazide (PMA) is a selective nucleic acid intercalating dye that can be combined with real‐time PCR (qPCR) in order to evaluate cell viability in food samples. The aim of the present work was to evaluate PMA‐qPCR to detect and quantify viable STEC cells in beef burgers using stx2 as target gene. First, it was determined that 100 µM of PMA could inhibit qPCR signal from non‐viable cells and had no influence on the amplification of different concentrations of viable cells. Then, it was shown that PMA efficiently distinguished between different log cfu of viable cells in presence of a high concentration of non‐viable cells, both in culture and in beef burger homogenates. Finally, it was determined that PMA could distinguish between viable and non‐viable cells within the same log cfu in beef burger homogenates. PMA‐qPCR effectively detected and quantified viable STEC cells in culture and in beef burger homogenates.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.relationinfo:eu-repograntAgreement/INTA/2019-PE-E7-I147-001/2019-PE-E7-I147-001/AR./INOCUIDAD DE ALIMENTOS PARA CONSUMO HUMANO Y ANIMALes_AR
dc.relationinfo:eu-repograntAgreement/INTA/PNAIyAV-1130042/AR./Herramientas y sistemas para la gestión de la calidad integral.
dc.rightsinfo:eu-repo/semantics/embargoedAccesses_AR
dc.sourceJournal Food Processing and Preservation 45 (3) : e15338 (2021)es_AR
dc.subjectEscherichia colies_AR
dc.subjectViabilityeng
dc.subjectMinced Meateng
dc.subjectCarne Picadaes_AR
dc.subject.otherBeef Burgerseng
dc.subject.otherHamburguesas de Terneraes_AR
dc.subject.otherPropidium monoazideeng
dc.subject.otherMonoazida de propidioes_AR
dc.subject.otherqPCRes_AR
dc.subject.otherPCR en tiempo real (qPCR)es_AR
dc.titleEvaluation of PMA‐qPCR methodology to detect and quantify viable Shiga toxin‐producing Escherichia coli in beef burgerses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/acceptedVersiones_AR
dc.description.origenInstituto de Investigación de Tecnología de Alimentoses_AR
dc.description.filFil: Rey, María De Los Ángeles. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina.es_AR
dc.description.filFil: Rey, María De Los Ángeles. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables (UEDD INTA CONICET); Argentina.es_AR
dc.description.filFil: Cap, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina.es_AR
dc.description.filFil: Cap, Mariana. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables (UEDD INTA CONICET); Argentina.es_AR
dc.description.filFil: Favre, Leonardo Cristian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina.es_AR
dc.description.filFil: Favre, Leonardo Cristian. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables (UEDD INTA CONICET); Argentina.es_AR
dc.description.filFil: Rodriguez, Anabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina.es_AR
dc.description.filFil: Rodriguez, Anabel. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables (UEDD INTA CONICET); Argentina.es_AR
dc.description.filFil: Dus Santos, Maria Jose. Instituto de Virología e Innovaciones tecnológicas. Centro de Investigaciones en Ciencias Veterinarias y Agronómicas (INTA-CONICET); Argentina.es_AR
dc.description.filFil: Vaudagna Sergio Ramon. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina.es_AR
dc.description.filFil: Vaudagna Sergio Ramon. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables (UEDD INTA CONICET); Argentina.es_AR
dc.description.filFil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina.es_AR
dc.description.filFil: Mozgovoj, Marina Valeria. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables (UEDD INTA CONICET); Argentina.es_AR
dc.subtypecientifico


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