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In this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra
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dc.contributor.author | Bacanelli, Gisele | |
dc.contributor.author | Olarte, Larissa C. | |
dc.contributor.author | Silva, Marcio Roberto | |
dc.contributor.author | Rodrigues, Rudielle A. | |
dc.contributor.author | Carneiro, Paulo A. M. | |
dc.contributor.author | Kannene, John B. | |
dc.contributor.author | Pasquatti, Taynara N. | |
dc.contributor.author | Takatani, Haruo | |
dc.contributor.author | Zumarraga, Martin Jose | |
dc.contributor.author | Etges, Rodrigo N. | |
dc.contributor.author | Araujo, Flabio Ribeiro de | |
dc.contributor.author | Verbisck, Newton V. | |
dc.date.accessioned | 2019-12-26T10:52:17Z | |
dc.date.available | 2019-12-26T10:52:17Z | |
dc.date.issued | 2019-10 | |
dc.identifier.issn | 1347-7439 | |
dc.identifier.other | https://doi.org/10.1292/jvms.19-0214 | |
dc.identifier.uri | https://www.jstage.jst.go.jp/article/jvms/81/10/81_19-0214/_article | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/6567 | |
dc.description.abstract | In this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra reference database were optimized for the identification of 80 clinical isolates of mycobacteria. As a result of this optimization, it was possible to identify and differentiate M. bovis from other members of the Mycobacterium tuberculosis complex with 100% specificity, 90.91% sensitivity and 91.25% reliability. MALDI-TOF MS methodology described herein provides successful identification of M. bovis within bovine/bubaline clinical samples, demonstrating its usefulness for bovine tuberculosis diagnosis in the future. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | The Japanese Society of Veterinary Science | es_AR |
dc.rights | info:eu-repo/semantics/openAccess | es_AR |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
dc.source | The Journal of Veterinary Medical Science 81 (10) : 1400-1408 (Octubre 2019) | es_AR |
dc.subject | Tuberculosis | es_AR |
dc.subject | Mycobacterium bovis Infections | eng |
dc.subject | Infeccion por Mycobacterium bovis | es_AR |
dc.subject | Ionization Treatment | eng |
dc.subject | Tratamiento Ionizante | es_AR |
dc.subject | Desorption | eng |
dc.subject | Desorción | es_AR |
dc.subject | Spectrometry | eng |
dc.subject | Espectrometría | es_AR |
dc.subject | Mycobacterium tuberculosis Infections | eng |
dc.subject | Infeccion Mycobacterium tuberculosis | es_AR |
dc.subject | Bovinae | eng |
dc.subject | Bovina | es_AR |
dc.title | Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
dc.description.origen | Instituto de Biotecnología | es_AR |
dc.description.fil | Fil: Bacanelli, Gisele. Federal University of Mato Grosso do Sul. Biotechnology and Biodiversity of the Central Western Region Postgraduate Program; Brasil | es_AR |
dc.description.fil | Fil: Olarte, Larissa C. Federal University of Mato Grosso do Sul. Biochemistry and Molecular Biology Multicentric Postgraduate Program; Brasil | es_AR |
dc.description.fil | Fil: Silva, Marcio Roberto. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Leite; Brasil | es_AR |
dc.description.fil | Fil: Rodrigues, Rudielle A. Federal University of Mato Grosso do Sul. Faculty of Veterinary Medicine. Veterinary Sciences Postgraduate Program; Brasil | es_AR |
dc.description.fil | Fil: Carneiro, Paulo A. M. Michigan State University. Center for Comparative Epidemiology; Estados Unidos | es_AR |
dc.description.fil | Fil: Kannene, John B. Michigan State University. Center for Comparative Epidemiology; Estados Unidos | es_AR |
dc.description.fil | Fil: Pasquatti, Taynara N. Dom Bosco Catholic University; Brasil | es_AR |
dc.description.fil | Fil: Takatani, Haruo. Agricultural Defense Agency of Amazonas; Brasil | es_AR |
dc.description.fil | Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Etges, Rodrigo N. Secretary of Agriculture, Livestock and Irrigation; Brasil | es_AR |
dc.description.fil | Fil: Araujo, Flabio Ribeiro de. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; Brasil | es_AR |
dc.description.fil | Fil: Verbisck, Newton V. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; Brasil | es_AR |
dc.subtype | cientifico |
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