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Resumen
In this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra [ver mas...]
dc.contributor.authorBacanelli, Gisele
dc.contributor.authorOlarte, Larissa C.
dc.contributor.authorSilva, Marcio Roberto
dc.contributor.authorRodrigues, Rudielle A.
dc.contributor.authorCarneiro, Paulo A. M.
dc.contributor.authorKannene, John B.
dc.contributor.authorPasquatti, Taynara N.
dc.contributor.authorTakatani, Haruo
dc.contributor.authorZumarraga, Martin Jose
dc.contributor.authorEtges, Rodrigo N.
dc.contributor.authorAraujo, Flabio Ribeiro de
dc.contributor.authorVerbisck, Newton V.
dc.date.accessioned2019-12-26T10:52:17Z
dc.date.available2019-12-26T10:52:17Z
dc.date.issued2019-10
dc.identifier.issn1347-7439
dc.identifier.otherhttps://doi.org/10.1292/jvms.19-0214
dc.identifier.urihttps://www.jstage.jst.go.jp/article/jvms/81/10/81_19-0214/_article
dc.identifier.urihttp://hdl.handle.net/20.500.12123/6567
dc.description.abstractIn this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra reference database were optimized for the identification of 80 clinical isolates of mycobacteria. As a result of this optimization, it was possible to identify and differentiate M. bovis from other members of the Mycobacterium tuberculosis complex with 100% specificity, 90.91% sensitivity and 91.25% reliability. MALDI-TOF MS methodology described herein provides successful identification of M. bovis within bovine/bubaline clinical samples, demonstrating its usefulness for bovine tuberculosis diagnosis in the future.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherThe Japanese Society of Veterinary Sciencees_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.sourceThe Journal of Veterinary Medical Science 81 (10) : 1400-1408 (Octubre 2019)es_AR
dc.subjectTuberculosises_AR
dc.subjectMycobacterium bovis Infectionseng
dc.subjectInfeccion por Mycobacterium bovises_AR
dc.subjectIonization Treatmenteng
dc.subjectTratamiento Ionizantees_AR
dc.subjectDesorptioneng
dc.subjectDesorciónes_AR
dc.subjectSpectrometryeng
dc.subjectEspectrometríaes_AR
dc.subjectMycobacterium tuberculosis Infectionseng
dc.subjectInfeccion Mycobacterium tuberculosises_AR
dc.subjectBovinaeeng
dc.subjectBovinaes_AR
dc.titleMatrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinaees_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.rights.licenseCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Bacanelli, Gisele. Federal University of Mato Grosso do Sul. Biotechnology and Biodiversity of the Central Western Region Postgraduate Program; Brasiles_AR
dc.description.filFil: Olarte, Larissa C. Federal University of Mato Grosso do Sul. Biochemistry and Molecular Biology Multicentric Postgraduate Program; Brasiles_AR
dc.description.filFil: Silva, Marcio Roberto. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Leite; Brasiles_AR
dc.description.filFil: Rodrigues, Rudielle A. Federal University of Mato Grosso do Sul. Faculty of Veterinary Medicine. Veterinary Sciences Postgraduate Program; Brasiles_AR
dc.description.filFil: Carneiro, Paulo A. M. Michigan State University. Center for Comparative Epidemiology; Estados Unidoses_AR
dc.description.filFil: Kannene, John B. Michigan State University. Center for Comparative Epidemiology; Estados Unidoses_AR
dc.description.filFil: Pasquatti, Taynara N. Dom Bosco Catholic University; Brasiles_AR
dc.description.filFil: Takatani, Haruo. Agricultural Defense Agency of Amazonas; Brasiles_AR
dc.description.filFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Etges, Rodrigo N. Secretary of Agriculture, Livestock and Irrigation; Brasiles_AR
dc.description.filFil: Araujo, Flabio Ribeiro de. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; Brasiles_AR
dc.description.filFil: Verbisck, Newton V. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; Brasiles_AR
dc.subtypecientifico


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