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The present work compared 2 culture methods and a PCR assay applied with 2 enrichment methods for the detection of motile and nonmotile Salmonella strains using artificially contaminated egg content. The specificity (Sp) was 1 in all methods. The sensitivity (Se), accuracy (Ac), positive predictive value (PPV), and negative predictive value (NPV) were 1 in both culture methods for motile and nonmotile strains. In reference to the PCR methods, Se and PPV [ver mas...]
dc.contributor.authorSoria, Mario
dc.contributor.authorSoria, Maria Cecilia
dc.contributor.authorBueno, Dante Javier
dc.date.accessioned2019-12-06T14:32:44Z
dc.date.available2019-12-06T14:32:44Z
dc.date.issued2012-10
dc.identifier.issn0032-5791
dc.identifier.issn1525-3171
dc.identifier.otherhttps://doi.org/10.3382/ps.2012-02253
dc.identifier.urihttps://academic.oup.com/ps/article/91/10/2668/1561306
dc.identifier.urihttp://hdl.handle.net/20.500.12123/6467
dc.description.abstractThe present work compared 2 culture methods and a PCR assay applied with 2 enrichment methods for the detection of motile and nonmotile Salmonella strains using artificially contaminated egg content. The specificity (Sp) was 1 in all methods. The sensitivity (Se), accuracy (Ac), positive predictive value (PPV), and negative predictive value (NPV) were 1 in both culture methods for motile and nonmotile strains. In reference to the PCR methods, Se and PPV were between 0 and 1, whereas Ac and NPV were between 0.14 and 1. The detection level of motile and nonmotile strains was 5 to 54 cfu per 25 mL for both culture methods, but some strains could not be detected by the PCR methods. Extending incubation time of the enrichment medium to 5 d in the tetrathionate broth (TT), and Muller-Kauffmann tetrathionate-novobiocin broth (MKTTn) methods did not improve the isolation rates. All selective plating media did not show any statistical differences in the parameters of performance studied. Kappa coefficients showed that there was an excellent agreement between the bacteriological methods for all Salmonella strains. The agreement was very good and good between the PCR methods, for motile and nonmotile strains, respectively. However, there was a poor agreement when the PCR and bacteriological methods were compared for motile and nonmotile Salmonella strains. The TT and MKTTn methods are similar in terms of Ac, Se, Sp, PPV, and NPV for different Salmonella strains in egg content. The use of the PCR method cannot improve the same parameters, described before, in this matrix. So, further studies are needed to improve the performance parameters and limit of detection in egg content for the PCR methods, so that test can be used in poultry and food industry.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherOxford Academic Presses_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourcePoultry Science 91 (10) : 2668–2676 (October 2012)es_AR
dc.subjectAves de Corrales_AR
dc.subjectPoultryeng
dc.subjectEnfermedades de los Animaleses_AR
dc.subjectAnimal Diseaseseng
dc.subjectSalmonellaes_AR
dc.subjectPCRes_AR
dc.subjectTécnicas de Cultivoes_AR
dc.subjectCulture Techniqueseng
dc.subjectHuevoses_AR
dc.subjectEggseng
dc.titleA comparative study of culture methods and polymerase chain reaction for Salmonella detection in egg contentes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Concepción del Uruguayes_AR
dc.description.filFil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentinaes_AR
dc.description.filFil: Soria, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentinaes_AR
dc.subtypecientifico


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