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Abstract
Cyclodextrins (CD) are cyclic oligosaccharides with multiple applications in the food, pharmaceutical, cosmetic, agricultural and chemical industries. In this work, the conditions used to produce CD with cyclodextrin glycosyltransferase from Bacillus circulans DF 9R were optimized using experimental designs. The developed method allowed the partial purification and concentration of the enzyme from the cultural broth and, subsequently, the CD production, [ver mas...]
dc.contributor.authorSzerman, Natalia
dc.contributor.authorSchroh, Ignacio
dc.contributor.authorRossi, Ana Lía
dc.contributor.authorRosso, Adriana Mabel
dc.contributor.authorKrymkiewicz, Norberto
dc.contributor.authorFerrarotti, Susana Alicia
dc.date.accessioned2019-09-24T11:20:16Z
dc.date.available2019-09-24T11:20:16Z
dc.date.issued2006-09-30
dc.identifier.issn0960-8524
dc.identifier.otherhttps://doi.org/10.1016/j.biortech.2006.09.056
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0960852406005281
dc.identifier.urihttp://hdl.handle.net/20.500.12123/5951
dc.description.abstractCyclodextrins (CD) are cyclic oligosaccharides with multiple applications in the food, pharmaceutical, cosmetic, agricultural and chemical industries. In this work, the conditions used to produce CD with cyclodextrin glycosyltransferase from Bacillus circulans DF 9R were optimized using experimental designs. The developed method allowed the partial purification and concentration of the enzyme from the cultural broth and, subsequently, the CD production, using the same cassava starch as enzyme adsorbent and as substrate. Heat-treatment of raw starch at 70 C for 15 min in the presence of adsorbed cyclodextrin glycosyltransferase allowed the starch liquefaction without enzyme inactivation. The optimum conditions for CD production were: 5% (w/v) cassava starch, 15 U of enzyme per gram of substrate, reaction temperature of 56 C and pH 6.4. After 4 h, the proportion of starch converted to CD reached 66% (w/w) and the weight ratio of a-CD:b-CD:c-CD was 1.00:0.70:0.16.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevier
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceBioresource Technology 98 (15) : 2886–2891 (November 2007).es_AR
dc.subjectCyclodextrinseng
dc.subjectCiclodextrinases_AR
dc.subjectBacillus circulanses_AR
dc.subject.otherCyclodextrins Productioneng
dc.subject.otherProducción de ciclodextrinases_AR
dc.subject.otherCyclodextrin glycosyltransferaseeng
dc.subject.otherBacillus circulans DF 9Res_AR
dc.titleCyclodextrin production by cyclodextrin glycosyltransferase from Bacillus circulans DF 9Res_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.filFil: Szerman, Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina.es_AR
dc.description.filFil: Schroh, Ignacio. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina.es_AR
dc.description.filFil: Rossi, Ana Lía. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina.es_AR
dc.description.filFil: Rosso, Adriana Mabel. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina.es_AR
dc.description.filFil: Krymkiewicz, Norberto. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina.es_AR
dc.description.filFil: Ferrarotti, Susana Alicia. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina.es_AR
dc.subtypecientifico


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