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Abstract
Antigens with aromatic amino groups can be conjugated to proteins by a diazo bond. This property was used to synthesise an antigen-biotin conjugate using bovine serum albumin (BSA) as a bridge, and tested this conjugate in an enzymoimmunoassay for clenbuterol detection. The assay included a highly specific antibody and the biotin-avidin amplification system. Its coefficient of variation was lower than 20% in the range of 100–8000 pg per well and the limit [ver mas...]
dc.contributor.authorSpotorno, Viviana Graciela
dc.contributor.authorTezón, Jorge Guillermo
dc.date.accessioned2018-11-27T14:44:34Z
dc.date.available2018-11-27T14:44:34Z
dc.date.issued2012-12
dc.identifier.issn0954-0105
dc.identifier.issn1465-3443
dc.identifier.otherhttps://doi.org/10.1080/09540105.2011.629314
dc.identifier.urihttps://www.tandfonline.com/doi/full/10.1080/09540105.2011.629314
dc.identifier.urihttp://hdl.handle.net/20.500.12123/3977
dc.description.abstractAntigens with aromatic amino groups can be conjugated to proteins by a diazo bond. This property was used to synthesise an antigen-biotin conjugate using bovine serum albumin (BSA) as a bridge, and tested this conjugate in an enzymoimmunoassay for clenbuterol detection. The assay included a highly specific antibody and the biotin-avidin amplification system. Its coefficient of variation was lower than 20% in the range of 100–8000 pg per well and the limit of detection was 6.4 pg. The synthesis of clenbuterol–BSA–biotin conjugate was reproducible, the reaction product easily purified and the amplification capacity could be set by adjusting the clenbuterol:biotin ratioeng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherTaylor & Francises_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceFood and Agricultural Immunology 23 (4) : 315-328 (December 2012)es_AR
dc.subjectTécnicas Inmunológicases_AR
dc.subjectImmunological Techniqueseng
dc.subjectAntígenoses_AR
dc.subjectAntigenseng
dc.subjectBiotinaes_AR
dc.subjectBiotineng
dc.subjectTécnicas Inmunoenzimáticases_AR
dc.subjectImmunoenzyme Techniqueseng
dc.subjectClenbuteroles_AR
dc.subject.otherInmunoensayoses_AR
dc.titleDevelopment of an antigen-biotin conjugate with a protein bridge and its application for the development of an enzyme immunoassayes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Tecnología de Alimentoses_AR
dc.description.filFil: Spotorno, Viviana Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentinaes_AR
dc.description.filFil: Tezon, Jorge Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentinaes_AR
dc.subtypecientifico


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