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Abstract
The Agouti gene (ASIP) is one of the most important genes for coat color determination in mammals. It has a complex structure with several promoters and alternative non-coding first exons that are transcribed into mRNAs with different 5́UTR. These mRNA isoforms regulate the temporal and spatial expression of the gene, producing diverse pigmentation patterns. Here, we studied ASIP transcriptional variants and their expression in the skin of llamas with [ver mas...]
dc.contributor.authorAnello, Melina
dc.contributor.authorDaverio, Maria Silvana
dc.contributor.authorRodriguez, S.S.
dc.contributor.authorRomero, Sandra Raquel
dc.contributor.authorRenieri, Carlo
dc.contributor.authorVidal Rioja, Lidia Beatriz
dc.contributor.authorDi Rocco, Florencia
dc.dateinfo:eu-repo/date/embargoEnd/2022-10-19
dc.date.accessioned2021-10-19T12:42:34Z
dc.date.available2021-10-19T12:42:34Z
dc.date.issued2021-10
dc.identifier.issn0378-1119
dc.identifier.otherhttps://doi.org/10.1016/j.gene.2021.146018
dc.identifier.urihttp://hdl.handle.net/20.500.12123/10524
dc.identifier.urihttps://www.sciencedirect.com/science/article/abs/pii/S0378111921006132
dc.description.abstractThe Agouti gene (ASIP) is one of the most important genes for coat color determination in mammals. It has a complex structure with several promoters and alternative non-coding first exons that are transcribed into mRNAs with different 5́UTR. These mRNA isoforms regulate the temporal and spatial expression of the gene, producing diverse pigmentation patterns. Here, we studied ASIP transcriptional variants and their expression in the skin of llamas with different coat color phenotypes. We also described the ASIP locus, including promoter usage and the splicing events that originate each transcript variant. Using 5́RACE-PCR we isolated seven ASIP transcripts with alternative 5’UTR, where exons 1A, 1A’, 1C, 1D, and a novel non-coding exon 1A” were identified. Additionally, new alternative spliced forms were found. The diversity of ASIP 5’UTRs is originated by a complex pattern of alternative promoter usage, multiple transcription start sites and splicing events that include exon skipping and alternative 3’ splicing site selection. We found that ASIP was highly expressed in llamas with white and brown phenotypes while black animals presented very low expression. The main responsible for this difference was a fusion transcript between ASIP and NCOA6 genes, which was present in the skin of white and brown llamas but not in the black ones. The rest of ASIP transcripts presented very low expression in the skin, indicating that the main regulation point for ASIP gene expression is at the transcriptional level. Nevertheless, the characteristics of the 5’UTRs sequences suggest that alternative transcripts could be regulated differently at the protein synthesis level.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.rightsinfo:eu-repo/semantics/embargoedAccesses_AR
dc.sourceGene : 146018 (Available online 14 October 2021)es_AR
dc.subjectLlamaes_AR
dc.subjectLlamaseng
dc.subjectFenotiposes_AR
dc.subjectPhenotypeseng
dc.subjectGenéticaes_AR
dc.subjectGeneticseng
dc.subjectExpresión Génicaes_AR
dc.subjectGene Expressioneng
dc.subject.otherAgouti Geneeng
dc.subject.otherASIP Geneeng
dc.titleThe ASIP gene in the Llama (Lama glama) : alternative transcripts, expression and relation with color phenotypeses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/acceptedVersiones_AR
dc.description.origenIPAF Región NOAes_AR
dc.description.filFil: Anello, M. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular; Argentinaes_AR
dc.description.filFil: Anello, M. Provincia de Buenos Aires. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular; Argentinaes_AR
dc.description.filFil: Anello, M. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentinaes_AR
dc.description.filFil: Daverio, Maria Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular; Argentinaes_AR
dc.description.filFil: Daverio, Maria Silvana. Provincia de Buenos Aires. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular; Argentinaes_AR
dc.description.filFil: Daverio, Maria Silvana. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentinaes_AR
dc.description.filFil: Daverio, Maria Silvana. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Biología; Argentines_AR
dc.description.filFil: Rodriguez, S.S. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular; Argentinaes_AR
dc.description.filFil: Rodriguez, S.S. Provincia de Buenos Aires. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular; Argentinaes_AR
dc.description.filFil: Rodriguez, S.S. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentinaes_AR
dc.description.filFil: Romero, Sandra Raquel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto De Investigación y Desarrollo Tecnológico para la Agricultura Familiar Región NOA; Argentinaes_AR
dc.description.filFil: Renieri, Carlo. University of Camerino. School of Pharmacy; Italiaes_AR
dc.description.filFil: Vidal Rioja, Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular; Argentinaes_AR
dc.description.filFil: Vidal Rioja, Lidia. Provincia de Buenos Aires. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular; Argentinaes_AR
dc.description.filFil: Vidal Rioja, Lidia. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentinaes_AR
dc.description.filFil: Di Rocco, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular; Argentinaes_AR
dc.description.filFil: Di Rocco, Florencia. Provincia de Buenos Aires. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular; Argentinaes_AR
dc.description.filFil: Di Rocco, Florencia. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentinaes_AR
dc.subtypecientifico


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