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Protection efficacy of Argentinian isolates of Mycobacterium avium subsp. paratuberculosis with different genotypes and virulence in a murine model
Abstract
Paratuberculosis is a chronic disease caused by Mycobacterium avium subsp. paratuberculosis (Map). The disease causes economic losses and, therefore, it is imperative to follow proper control strategies, which should include an effective vaccine. Several strategies have assessed the virulence and immune response of Map strains that could be used as a vaccine. This study evaluates the degree of virulence, immune response, and protection of Argentinian
[ver mas...]
Paratuberculosis is a chronic disease caused by Mycobacterium avium subsp. paratuberculosis (Map). The disease causes economic losses and, therefore, it is imperative to follow proper control strategies, which should include an effective vaccine. Several strategies have assessed the virulence and immune response of Map strains that could be used as a vaccine. This study evaluates the degree of virulence, immune response, and protection of Argentinian strains of Map with different genotype in a murine model. Four local isolates (Cattle type) with different genotypes (analyzed by MIRU-VNTR and SSRs) were selected and evaluated in a virulence assay in BALB/c mice. This assay allowed us to differentiate virulent and low-virulence Map strains. The less virulent strains (1543/481 and A162) failed to induce a significant production of the proinflammatory cytokine IFNg, whereas the virulent strain 6611 established infection along with a proinflammatory immune response. On the other hand, the virulent strain 1347/498 was efficient in establishing a persistent infection, but failed to promote an important Th1 response compared with 6611 at the evaluated time. We selected the low-virulence strain 1543/498 as a live vaccine and the virulent strain 6611 as a live and inactivated vaccine in a protection assay in mice. Strain 1543/481 failed to protect the animals from challenge, whereas strain 6611, in its live and inactivated form, significantly reduced the CFUs count in the infected mice, although they had different immunological response profiles. The inactivated virulent strain 6611 is a potential vaccine candidate against paratuberculosis to be tested in cattle.
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![Thumbnail](/bitstream/handle/20.500.12123/4635/cover_Research%20in%20Veterinary%20Science_2018.gif?sequence=3&isAllowed=y)
Author
Colombatti Olivieri, Maria Alejandra;
Moyano, Roberto Damian;
Traveria, Gabriel Eduardo;
Alvarado Pinedo, Maria Fiorella;
Mon, Maria Laura;
Gravisaco, María José;
Delgado, Fernando Oscar;
Santangelo, María De La Paz;
Romano, Maria Isabel;
Fuente
Research in veterinary science 121 : 4-11. (December 2018)
Date
2018-12
Editorial
Elsevier
ISSN
0034-5288
Formato
pdf
Tipo de documento
artículo
Palabras Claves
Derechos de acceso
Restringido
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Metadata
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