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Study on the interference of vaccine antibodies with the serological diagnosis of leptospirosis in cattle
Abstract
A simple IgG-specific ELISA for Leptospira spp. was compared with the microscopic agglutination test (MAT) to detect IgG antibody responses to a commercial vaccine in cattle. We used an enzyme-linked immunosorbent assay (ELISA) with sonicated Leptospira interrogans serovar copenhageni M 20. After initial vaccination, specific antibodies against Leptospira spp. were detected in 90% of the animals by IgG-ELISA and 60% by MAT, while after booster, antibodies
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A simple IgG-specific ELISA for Leptospira spp. was compared with the microscopic agglutination test (MAT) to detect IgG antibody responses to a commercial vaccine in cattle. We used an enzyme-linked immunosorbent assay (ELISA) with sonicated Leptospira interrogans serovar copenhageni M 20. After initial vaccination, specific antibodies against Leptospira spp. were detected in 90% of the animals by IgG-ELISA and 60% by MAT, while after booster, antibodies were detected in 100% and 90% of the animals by IgG-ELISA and MAT, respectively. Both serological MAT and ELISA tests revealed interferences of vaccine antibodies. Disease diagnosis with ELISA and MAT methods should be made two and a half months and four months, respectively, after vaccination to avoid interference of vaccine antibodies. On the other hand, our results suggest that IgG-ELISA may be a useful method to assess the development of IgG antibodies induced by Leptospira vaccine.
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![Thumbnail](/bitstream/handle/20.500.12123/18583/VeterinaryMicrobiology-cover.gif?sequence=4&isAllowed=y)
Fuente
Veterinary Microbiology : 110169 (Available online 9 July 2024)
Date
2024
Editorial
Elsevier
ISSN
0378-1135
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