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Comparison of ELISA using recombinant LipL32 and sonicated antigen of leptospira for detecting bovine leptospirosis
Martinez, Mara Leila; Rodriguez, Marcelo A.; Saraullo, Vanina Rosa; Irazu, Lucía E.; Hamer, Micaela; Watanabe, Olivia; Grune Loffler, Sylvia; Romero, Graciela Noemi; Samartino, Luis Ernesto; Brihuega, Bibiana Felicitas (Elsevier, 2022-01)Leptospirosis is one of the most widely distributed zoonosis in the world. Bovine leptospirosis is a serious problem in bovine production, causing reproductive losses. The aim of this work was to compare recombinant LipL32 ... -
Detección de Leptospira spp. (Spirochaetales: Leptospiraceae) en muestras ambientales de regiones habitadas por poblaciones vulnerables del norte argentino
Grune Loffler, Sylvia; Periago, María Victoria; Watanabe, Olivia; Saraullo, Vanina Rosa; Aldama, Evangelina; Cejas, Graciela; Cruz, Diego; Delgado, Cintia; Goizueta, Carolina; Hamer, Micaela; Martinez, Mara Leila; Brihuega, Bibiana Felicitas (Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, 2021-12)La leptospirosis continúa siendo hoy en día un problema para la salud pública, principalmente en poblaciones de bajos recursos socioeconómicos. En este trabajo se presenta la detección de leptospiras patógenas en muestras ... -
First report of pathogenic Leptospira spp. in Tadarida brasiliensis bats (family Molossidae) and Eptesicus furinalis (family Vespertilionidae) of Argentina. New host species in this country? = Primer reporte de Leptospira spp. patógena en murciélagos Tadarida brasiliensis (familia: Molossidae) y Eptesicus furinalis (familia: Vespertilionidae) en Argentina. ¿Nuevas especies hospedadoras en el país?
Saraullo, Vanina Rosa; Grune Loffler, Sylvia; Pastorino, Florencia; Watanabe, Olivia; Alonso, Maria Laura; Hamer, Micaela; Moreira, Cecilia; Martinez, Mara Leila; Martinez, Gustavo; Brihuega, Bibiana Felicitas (Elsevier, 2021-09)Leptospirosis is an endemic disease caused by Leptospira spp., a bacterium that affects animals and humans. In recent years, the number of reports of leptospirosis in wild animals has increased, which highlights the need ... -
New enzyme-linked immunoassay for the detection of specific antibodies against multiple Leptospira serogroups in bovine sera
Martinez, Mara Leila; Rodriguez, Marcelo A.; Irazu, Lucía E.; Romero, Graciela Noemi; Saraullo, Vanina Rosa; Watanabe, Olivia; Hamer, Micaela; Grune Loffler, Sylvia; Samartino, Luis Ernesto; Brihuega, Bibiana Felicitas (Elsevier, 2021-04)Leptospirosis is a zoonotic disease with worldwide endemicity in Argentina, it is a significant public health problem in low-income populations. Bovine leptospirosis is a serious economic problem for cattle production, ... -
Optimization and comparative analysis of LAMP and PCR techniques for the detection of leptospiral DNA in Golden Syrian hamsters
Hamer, Micaela; Watanabe, Olivia; Saraullo, Vanina Rosa; Ortega, Facundo Miguel; Sanchez, Maria Cristina; Martinez, Mara Leila; Brihuega, Bibiana Felicitas; Grune Loffler, Sylvia (Springer, 2024-03)Leptospirosis is a zoonotic disease with significant public health and economic impact worldwide. Rapid and accurate diagnosis is essential for effective prevention and treatment. This study optimized a loop-mediated ... -
Study on the interference of vaccine antibodies with the serological diagnosis of leptospirosis in cattle
Martinez, Mara Leila; Esteban, Micaela; Sanchez, Maria Cristina; Saraullo, Vanina Rosa; Hamer, Micaela; Samartino, Luis Ernesto; Brihuega, Bibiana Felicitas (Elsevier, 2024)A simple IgG-specific ELISA for Leptospira spp. was compared with the microscopic agglutination test (MAT) to detect IgG antibody responses to a commercial vaccine in cattle. We used an enzyme-linked immunosorbent assay ... -
Use of Chelex-100 for the molecular diagnosisof five animal pathogens
Tomazic, Mariela Luján; Hamer, Micaela; Bustos, Carla Paola; Arregui, Matias Ezequiel; Ascencio, Mariano; Saraullo, Vanina Rosa; Watanabe, Olivia; Brihuega, Bibiana Felicitas; Rodriguez, Anabel Elisa; Grune Loffler, Sylvia (Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, 2021-06)Molecular tools have improved conventional veterinary diagnosis. Acid nucleic extraction is a key step for downstream applications. This work aimed to compare the DNA extraction method Chelex-100 resin (M1) ... -
Use of the Leptospira sp. ligB C-terminus coding region as a diagnostic tool of animal leptospirosis
Saraullo, Vanina Rosa; Grune Loffler, Sylvia; Florin-Christensen, Mónica; Watanabe, Olivia; Hamer, Micaela; Martinez, Mara Leila; Brihuega, Bibiana Felicitas (Elsevier, 2021-10)Leptospirosis is the most widespread zoonosis worldwide, and it can cause reproductive failures in livestock, while in humans may vary from a mild fever to multi-organ failure and death. Due to this, in this study, we ...