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Multiple ways to evade the bacteriostatic action of glyphosate in rhizobia include the mutation of the conserved serine 90 of the nitrogenase subunit NifH to alanine

Abstract
The genome resequencing of spontaneous glyphosate-resistant mutants derived from the soybean inoculant E109 allowed identifying genes most likely associated with the uptake (gltL and cya) and metabolism (zigA and betA) of glyphosate, as well as with nitrogen fixation (nifH). Mutations in these genes reduce the lag phase and improve nodulation under glyphosate stress. In addition to providing glyphosate resistance, the amino acid exchange Ser90Ala in NifH [ver mas...]
The genome resequencing of spontaneous glyphosate-resistant mutants derived from the soybean inoculant E109 allowed identifying genes most likely associated with the uptake (gltL and cya) and metabolism (zigA and betA) of glyphosate, as well as with nitrogen fixation (nifH). Mutations in these genes reduce the lag phase and improve nodulation under glyphosate stress. In addition to providing glyphosate resistance, the amino acid exchange Ser90Ala in NifH increased the citrate synthase activity, growth rate and plant growth-promoting efficiency of E109 in the absence of glyphosate stress, suggesting roles for this site during both the free-living and symbiotic growth stages. [Cerrar]
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Author
Liebrenz, Karen Ivana;   Frare, Romina Alejandra;   Gomez, Maria Cristina;   Pascuan, Cecilia Gabriela;   Brambilla, Silvina Maricel;   Soldini, Diego Omar;   Maguire, Vanina;   Carrio, Alejandro Javier;   Ruiz, Oscar;   McCormick, Wayne;   Soto, Gabriela Cynthia;   Ayub, Nicolás Daniel;  
Fuente
Research in Microbiology 173 (6-7) : 103952 (Julio-Septiembre 2022)
Date
2022-07
Editorial
Elsevier
ISSN
1769-7123
URI
http://hdl.handle.net/20.500.12123/12422
https://www.sciencedirect.com/science/article/abs/pii/S092325082200033X
DOI
https://doi.org/10.1016/j.resmic.2022.103952
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Palabras Claves
Bradyrhizobium; Soybeans; Soja; Glyphosate; Glifosato; Nitrogenase; Nitrogenasa; Disinfectants; Desinfectantes; Rhizobiaceae; Alanine; Alanina; Nitrogen Fixation; Fijación del Nitrógeno; Mutation; Mutación; Stress; Estres;
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