autores 

Autores

Mostrando ítems 1-19 de 19

    • Caracterización funcional del gen PhaZ en plantas 

      Ortiz, Ana Veronica; Frare, Romina Alejandra; Gomez, Maria Cristina; Jozefkowicz, Cintia; Ayub, Nicolás Daniel; Blumwald, Eduardo; Soto, Gabriela Cynthia (Ministerio de Desarrollo Agrario - Instituto Tecnológico de Chascomús, 2025-05)
      La germinación de semillas es un proceso biológico complejo que involucra una serie de eventos, los cuales se dividen en tres fases principales: la imbibición (fase I), la activación metabólica (fase II) y la protrusión ...

    • CRISPR/Cas9-mediated knockout of a polyester synthase-like gene delays flowering time in alfalfa 

      Galindo Sotomonte, Luisa Fernanda; Jozefkowicz, Cintia; Gomez, Maria Cristina; Stritzler, Margarita; Frare, Romina Alejandra; Bottero, Ana Emilia; Tajima, Hiromi; Blumwald, Eduardo; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Springer, 2023-02)
      Alfalfa (Medicago sativa), commonly known as the “Queen of Forages”, is the main source of plant protein for meat and milk production systems worldwide. Since alfalfa is a perennial vegetative-harvest forage, delaying ...

    • Efficient CRISPR/Cas9 genome editing in alfalfa using a public germplasm 

      Bottero, Ana Emilia; Massa, Gabriela Alejandra; Gonzalez, Matías Nicolás; Stritzler, Margarita; Tajima, Hiromi; Gomez, Maria Cristina; Frare, Romina Alejandra; Feingold, Sergio Enrique; Blumwald, Eduardo; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Frontiers Media, 2021-07)
      Because its ability to acquire large amounts of nitrogen by symbiosis, tetraploid alfalfa is the main source of vegetable proteins in meat and milk production systems in temperate regions. Alfalfa cultivation also adds ...

    • Engineering a commercial soybean inoculant to efficiently degrade glyphosate 

      Brambilla, Silvina Maricel; Pascuan, Cecilia Gabriela; Frare, Romina Alejandra; Liebrenz, Karen Ivana; Pesquero, Natalia; Ruiz, María Juana; Soto, Gabriela Cynthia; Cristos, Diego Sebastian; Ayub, Nicolás Daniel (Elsevier, 2025-06)
      Nitrogen-fixing rhizobia possess the well-characterized phn operon, which encodes the C–P lyase enzymatic complex responsible for degrading phosphonates such as glyphosate. However, glyphosate exerts a bacteriostatic effect ...

    • Exploring the role of the NO‑detoxifying enzyme HmpA in the evolution of domesticated Alfalfa Rhizobia 

      Frare, Romina Alejandra; Pascuan, Cecilia Gabriela; Galindo Sotomonte, Luisa Fernanda; McCormick, Wayne; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Springer, 2022-02)
      We have previously shown the extensive loss of genes during the domestication of alfalfa rhizobia and the high nitrous oxide emission associated with the extreme genomic instability of commercial inoculants. In the present ...

    • Generation of a multi‑herbicide‑tolerant alfalfa by using base editing 

      Bottero, Ana Emilia; Gomez, Maria Cristina; Stritzler, Margarita; Tajima, Hiromi; Frare, Romina Alejandra; Pascuan, Cecilia Gabriela; Blumwald, Eduardo; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Springer, 2022-02)
      We present the first report on base editing in alfalfa. Specifically, we showed edited alfalfa with tolerance to both sulfonylurea- and imidazolinone-type herbicides. Alfalfa (Medicago sativa) is one of the most important ...

    • Genomic and physiological plasticity in natural variants of commercial soybean inoculants supports the non-GMO status of base-edited inoculants 

      Cardillo, Maria Eugenia; Brambilla, Silvina Maricel; Liebrenz, Karen Ivana; Frare, Romina Alejandra; Maguire, Vanina Giselle; Soto, Gabriela Cynthia; Ruiz, Oscar Adolfo; Ayub, Nicolás Daniel (Springer, 2025-04)
      The regulatory classification of genome-edited microorganisms remains a topic of debate, particularly in the agricultural sector. In this study, we analyze the genomic and physiological differences of commercial variants ...

    • Improvement of anti-bloating alfalfa 

      Cardillo, Maria Eugenia; Frare, Romina Alejandra; Ayub, Nicolás Daniel; Pascuan, Cecilia Gabriela; Soto, Gabriela Cynthia (Sociedad Argentina de Fisiología Vegetal, 2025-08)
      Alfalfa (Medicago sativa L.) is one of the most important legumes for the stability of agricultural-livestock production systems due to its excellent forage quality, adaptability to different climate and soil, high biomass ...

    • Major cereal crops benefit from biological nitrogen fixation when inoculated with the nitrogen-fixing bacterium Pseudomonas protegens Pf-5 X940 

      Fox, Ana Romina; Soto, Gabriela Cinthia; Valverde, Claudio Fabián; Russo, Daniela Marta; Lagares, Antonio; Zorreguieta, Ángeles; Pascuan, Cecilia Gabriela; Frare, Romina Alejandra; Mercado Blanco, Jesús; Dixon, Ray; Ayub, Nicolás Daniel; Alleva, Karina Edith (2016)
      A main goal of biological nitrogen fixation research has been to expand the nitrogen-fixing ability to major cereal crops. In this work, we demonstrate the use of the efficient nitrogen-fixing rhizobacterium Pseudomonas ...

    • Microevolution rather than large genome divergence determines the effectiveness of legume–rhizobia symbiotic interaction under field conditions 

      Jozefkowicz, Cintia; Brambilla, Silvina Maricel; Frare, Romina Alejandra; Stritzler, Margarita; Puente, Mariana Laura; Piccinetti, Carlos Fabian; Soto, Gabriela Cinthia; Ayub, Nicolás Daniel (2017-08)
      Despite the vast screening for natural nitrogenfixing isolates by public and private consortia, no significant progresses in the production of improved nitrogenfixing inoculants for alfalfa production have been made in the ...

    • mRNA biogenesis-related helicase eIF4AIII from Arabidopsis thaliana is an important factor for abiotic stress adaptation 

      Pascuan, Cecilia Gabriela; Frare, Romina Alejandra; Alleva, Karina Edith; Ayub, Nicolás Daniel; Soto, Gabriela Cinthia (2016)
      Similar to other plant species, Arabidopsis has a huge repertoire of predicted helicases, including the eIF4AIII factor, a putative component of the exon junction complex related to mRNA biogenesis. In this article, we ...

    • Multiple ways to evade the bacteriostatic action of glyphosate in rhizobia include the mutation of the conserved serine 90 of the nitrogenase subunit NifH to alanine 

      Liebrenz, Karen Ivana; Frare, Romina Alejandra; Gomez, Maria Cristina; Pascuan, Cecilia Gabriela; Brambilla, Silvina Maricel; Soldini, Diego Omar; Maguire, Vanina; Carrio, Alejandro Javier; Ruiz, Oscar; McCormick, Wayne; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Elsevier, 2022-07)
      The genome resequencing of spontaneous glyphosate-resistant mutants derived from the soybean inoculant E109 allowed identifying genes most likely associated with the uptake (gltL and cya) and metabolism (zigA and betA) of ...

    • Oligopeptide uptake improves oxidative stress resistance and nodulation competitiveness in soybean rhizobia 

      Brambilla, Silvina Maricel; Liebrenz, Karen Ivana; Frare, Romina Alejandra; Gomez, Maria Cristina; Stritzler, Margarita; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Elsevier, 2023-12)
      We here explored the mechanisms associated with oxidative stress resistance and root colonization by soybean rhizobia. Genomic and physiological analyses supported the fact that the mutation of a highly conserved valine ...

    • Rapid and cloning‑free screening of edited alfalfa via next‑generation sequencing 

      Stritzler, Margarita; Pascuan, Cecilia Gabriela; Bottero, Ana Emilia; Gomez, Maria Cristina; Frare, Romina Alejandra; Puebla, Andrea Fabiana; Tajima, Hiromi; Ayub, Nicolás Daniel; Blumwald, Eduardo; Soto, Gabriela Cynthia (Springer, 2022-07)
      The identification of full knockout plants is frequently the rate-limiting step of the research and commercial projects searching the complete loss of the function of target genes via the CRISPR/Cas9 system. This problem ...

    • Retrotransposon and CRISPR/Cas9‑mediated knockout of NOD26 impairs the legume‑rhizobia symbiosis 

      Frare, Romina Alejandra; Stritzler, Margarita; Gomez, Maria Cristina; Tajima, Hiromi; Pascuan, Cecilia Gabriela; López Fernández, María Paula; Bottero, Ana Emilia; Nikel, Pablo I.; Alleva, Karina Edith; Ayub, Nicolás Daniel; Blumwald, Eduardo; Soto, Gabriela Cynthia (Springer, 2022-07)
      The multifunctional channel NOD26, identified and extensively studied (both biochemically and biophysically) in soybean, is a major protein component of the symbiosome membrane. The water and ammonia transport activities ...

    • Stable symbiotic nitrogen fixation under water-deficit field conditions by a stress-tolerant alfalfa microsymbiont and its complete genome sequence : Short communication 

      Jozefkowicz, Cintia; Brambilla, Silvina Maricel; Frare, Romina Alejandra; Stritzler, Margarita; Piccinetti, Carlos Fabian; Puente, Mariana Laura; Berini, Carolina Andrea; Reyes Perez, Pedro Jose; Soto, Gabriela Cinthia; Ayub, Nicolás Daniel (2017-12)
      We here characterized the stress-tolerant alfalfa microsymbiont Sinorhizobium meliloti B401. B401-treated plants showed high nitrogen fixation rates under humid and semiarid environments. The production of glycine betaine ...

    • Subfunctionalization probably drives the emergence of plant growth‑promoting genes 

      Frare, Romina Alejandra; Mozzicafreddo, Matteo; Gomez, Maria Cristina; Fussoni, Nerina Belen; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Springer, 2022-07)
      The duplication and divergence of stress-related genes might help microbes adapt to different environments. However, little is known about this type of subfunctionalization process in microbes. We here examined the evolution ...

    • Synthetic multi-antibiotic resistant plasmids in plant-associated bacteria from agricultural soils 

      Brambilla, Silvina Maricel; Frare, Romina Alejandra; Stritzler, Margarita; Soto, Gabriela Cynthia; Berini, Carolina Andrea; Jozefkowicz, Cintia; Ayub, Nicolás Daniel (Elsevier, 2020-09)
      Objectives: Unlike higher organisms such as domestic animals and cultivated plants, which display a robust reproductive isolation and limited dispersal ability, microbes exhibit an extremely promiscuous gene flow and can ...

    • Whole-Genome Resequencing of Spontaneous Oxidative Stress-Resistant Mutants Reveals an Antioxidant System of Bradyrhizobium japonicum Involved in Soybean Colonization 

      Liebrenz, Karen Ivana; Gomez, Maria Cristina; Brambilla, Silvina Maricel; Frare, Romina Alejandra; Stritzler, Margarita; Maguire, Vanina; Ruiz, Oscar; Soldini, Diego Omar; Pascuan, Cecilia Gabriela; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Springer, 2021-11)
      Soybean is the most inoculant-consuming crop in the world, carrying strains belonging to the extremely related species Bradyrhizobium japonicum and Bradyrhizobium diazoefficiens. Currently, it is well known that B. japonicum ...