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A synthesis of functional contributions of rhizobacteria to growth promotion in diverse crops
Brambilla, Silvina Maricel; Stritzler, Margarita; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Elsevier, 2022-12)The endophytic microbes are known to live asymptomatically inside their host and play crucial roles in the growth, development, fitness, and diversification of plants. The plant-endophyte association ranges from mutualism ... -
Absence of the nitrous oxide reductase gene cluster in commercial alfalfa inoculants is probably due to the extensive loss of genes during rhizobial domestication
Brambilla, Silvina Maricel; Frare, Romina Alejandra; Soto, Gabriela Cinthia; Jozefkowicz, Cintia; Ayub, Nicolás Daniel (Springer, 2018-08)As other legume crops, alfalfa cultivation increases the emission of the greenhouse gas nitrous oxide (N2O). Since legumesymbiotic nitrogen-fixing bacteria play a crucial role in this emission, it is important to understand ... -
Characterization of genetic diversity in accessions of prunus salicina lindl : keeping fruit flesh color ideotype while adapting to water stressed environments
Acuña, Cintia Vanesa; Rivas, Juan Gabriel; Brambilla, Silvina Maricel; Cerrillo, Teresa; Frusso, Enrique; Garcia, Martin Nahuel; Villalba, Pamela Victoria; Aguirre, Natalia Cristina; Sabio Y Garcia, Julia Veronica; Martinez, Maria Carolina; Hopp, Horacio Esteban; Marcucci Poltri, Susana Noemi (MDPI, 2019-08)The genetic diversity of 14 Japanese plum (Prunus salicina Lindl) landraces adapted to an ecosystem of alternating flooding and dry conditions was characterized using neutral simple sequence repeat (SSR) markers. Twelve ... -
Culturable bacteria associated with Anastrepha fraterculus sp. 1 : in search of nitrogen-fixing symbionts with biotechnological potential
Salgueiro, Julieta; Nussenbaum, Ana Laura; Marchesini, María Inés; Garbalena, Micaela Soledad; Brambilla, Silvina Maricel; Belliard, Silvina Ahnahi; Cuadros, Fabián; Núñez, Mauricio; Yáñez, Carolina; Juárez, María Laura; Vera, María Teresa; Lanzavecchia, Silvia Beatriz; Tsiamis, George; Segura, Diego Fernando (Wiley, 2025-02)Anastrepha fraterculus is a significant fruit fly pest in Argentina and other South American countries. Previous studies showed the key role of gut bacteria in the protection and nutrient assimilation of fruit flies, ... -
Edición génica del inoculante comercial de Alfalfa mediante base-editing por CRISPR-Cas9
Serantes, María Laura; Stritzler, Margarita; Brambilla, Silvina Maricel; Ayub, Nicolás Daniel (Ministerio de Desarrollo Agrario - Instituto Tecnológico de Chascomús, 2025-05)En países agroexportadores como Argentina, impulsar cultivos sostenibles con menos agroquímicos es clave para reducir costos ambientales y económicos. En este contexto, los inoculantes fijadores de nitrógeno son esenciales ... -
Edición génica en inoculantes de soja por CRISPR-Cas9 : Mejoramientos para una mayor sostenibilidad agrícola
Serantes, María Laura; Stritzler, Margarita; Brambilla, Silvina Maricel; Ayub, Nicolás Daniel (INTA, 2024-11)La promoción de cultivos sostenibles con menor uso de agroquímicos es esencial en la agricultura moderna. Los inoculantes fijadores de nitrógeno, como Bradyrhizobium japonicum E109 y SEMIA 5079, utilizados en soja, reducen ... -
Efectos de la domesticación de los rizobios simbiontes de alfalfa, sobre su capacidad de fijar nitrógeno y degradar óxido nitroso
Brambilla, Silvina Maricel (Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, 2020)La disponibilidad de nitrógeno es un factor crítico para la productividad de los cultivos. La aplicación de fertilizantes nitrogenados sintéticos derivados del petróleo ha logrado disminuir parcialmente la deficiencia de ... -
Engineering a commercial soybean inoculant to efficiently degrade glyphosate
Brambilla, Silvina Maricel; Pascuan, Cecilia Gabriela; Frare, Romina Alejandra; Liebrenz, Karen Ivana; Pesquero, Natalia; Ruiz, María Juana; Soto, Gabriela Cynthia; Cristos, Diego Sebastian; Ayub, Nicolás Daniel (Elsevier, 2025-06)Nitrogen-fixing rhizobia possess the well-characterized phn operon, which encodes the C–P lyase enzymatic complex responsible for degrading phosphonates such as glyphosate. However, glyphosate exerts a bacteriostatic effect ... -
Evaluación de un consorcio bacteriano para la fijación biológica de nitrógeno en alfalfa = Evaluation of a bacterial consortium for the nitrogen biological fixation in alfalfa
Moreno, Maria Valeria; Silva, Mercedes Pilar; Scarponi Gomez, Antonela; Arolfo, Valeria; Odorizzi, Ariel; Brambilla, Silvina Maricel; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel; López, J.; Erdozain Bagolin, Sofia Agostina; Lagares, Antonio (Asociación Argentina de Producción Animal (AAPA), 2024-09)La alfalfa (Medicago sativa L.) es un cultivo de importancia mundial por su alta calidad nutricional, alto rendimiento y su capacidad para la fijación biológica de nitrógeno atmosférico (FBN). En el proceso de FBN, la ... -
Exploring the cooperation between nitrogen-fixing and non-fixing alfalfa rhizobia
Brambilla, Silvina Maricel; Liebrenz, Karen Ivana; Odorizzi, Ariel; Arolfo, Valeria; Maguire, Vanina Giselle; Moreno, Maria Valeria; Ruiz, Oscar Adolfo; Stritzler, Margarita; Serantes, Maria Laura; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Springer, 2024-07-05)Alfalfa plays a crucial role in agriculture due to its nitrogen-fixing partnership with rhizobia, resulting in nutritious forage. While the potential parasitic action of inefficient rhizobia has been extensively studied, ... -
Exploring the relationship of potential new rhizobacteria metabolism with Lotus spp. performance in a rice-pasture rotation system
Maguire, Vanina Giselle; Rodríguez, Andrés Alberto; Alonso, Andrés Mariano; Ezquiaga, Juan Pedro; Salas, Nehuén; Llames, María Eugenia; Gortari, Maximiliano; Ayub, Nicolás Daniel; Brambilla, Silvina Maricel; Bouilly, Pedro José; Romero, Fernando Matías; Gárriz, Andrés; Ruíz, Oscar Adolfo (Springer, 2025-11-03)The first objective of this study was to identify and characterize new strains isolated from rice–pasture rotation soils, capable of: (A) establishing efficient symbiosis with Lotus plants and (B) enhancing their performance ... -
Genome editing of soybean inoculant using CRISPR/Cas9 system: enhancing agricultural sustainability
Serantes, Maria Laura; Stritzler, Margarita; Brambilla, Silvina Maricel; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Springer, 2024-04)In modern agriculture, inoculants, consisting of various bacteria and fungi, are essential for promoting crop growth and sustainability while reducing reliance on agrochemicals. Despite their benefits, there have been no ... -
Genomic and physiological plasticity in natural variants of commercial soybean inoculants supports the non-GMO status of base-edited inoculants
Cardillo, Maria Eugenia; Brambilla, Silvina Maricel; Liebrenz, Karen Ivana; Frare, Romina Alejandra; Maguire, Vanina Giselle; Soto, Gabriela Cynthia; Ruiz, Oscar Adolfo; Ayub, Nicolás Daniel (Springer, 2025-04)The regulatory classification of genome-edited microorganisms remains a topic of debate, particularly in the agricultural sector. In this study, we analyze the genomic and physiological differences of commercial variants ... -
Microevolution rather than large genome divergence determines the effectiveness of legume–rhizobia symbiotic interaction under field conditions
Jozefkowicz, Cintia; Brambilla, Silvina Maricel; Frare, Romina Alejandra; Stritzler, Margarita; Puente, Mariana Laura; Piccinetti, Carlos Fabian; Soto, Gabriela Cinthia; Ayub, Nicolás Daniel (2017-08)Despite the vast screening for natural nitrogenfixing isolates by public and private consortia, no significant progresses in the production of improved nitrogenfixing inoculants for alfalfa production have been made in the ... -
Multiple ways to evade the bacteriostatic action of glyphosate in rhizobia include the mutation of the conserved serine 90 of the nitrogenase subunit NifH to alanine
Liebrenz, Karen Ivana; Frare, Romina Alejandra; Gomez, Maria Cristina; Pascuan, Cecilia Gabriela; Brambilla, Silvina Maricel; Soldini, Diego Omar; Maguire, Vanina; Carrio, Alejandro Javier; Ruiz, Oscar; McCormick, Wayne; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Elsevier, 2022-07)The genome resequencing of spontaneous glyphosate-resistant mutants derived from the soybean inoculant E109 allowed identifying genes most likely associated with the uptake (gltL and cya) and metabolism (zigA and betA) of ... -
Obtención de bioinoculantes tolerantes a herbicidas para incrementar la productividad y mejorar la inocuidad ambiental de los cultivos de soja
Liebrenz, Karen Ivana; Brambilla, Silvina Maricel; Gomez, Maria Cristina; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (INTA, 2022-11)El glifosato inhibe una enzima involucrada en la síntesis de los aminoácidos aromáticos afectando la funcionalidad de los rizobios endosimbiontes de soja, disminuyendo la fijación de nitrógeno la productividad de los ... -
Oligopeptide uptake improves oxidative stress resistance and nodulation competitiveness in soybean rhizobia
Brambilla, Silvina Maricel; Liebrenz, Karen Ivana; Frare, Romina Alejandra; Gomez, Maria Cristina; Stritzler, Margarita; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Elsevier, 2023-12)We here explored the mechanisms associated with oxidative stress resistance and root colonization by soybean rhizobia. Genomic and physiological analyses supported the fact that the mutation of a highly conserved valine ... -
Spontaneous Mutations in the Nitrate Reductase Gene napC Drive the Emergence of Eco-friendly Low-N2O-Emitting Alfalfa Rhizobia in Regions with Different Climates
Brambilla, Silvina Maricel; Soto, Gabriela; Odorizzi, Ariel; Arolfo, Valeria; McCormick, Wayne; Primo, Emiliano; Giordano, Walter; Jozefkowicz, Cintia; Ayub, Nicolás Daniel (Springer, 2020)We have recently shown that commercial alfalfa inoculants (e.g., Sinorhizobium meliloti B399), which are closely related to the denitrifier model strain Sinorhizobium meliloti 1021, have conserved nitrate, nitrite, and ... -
Stable symbiotic nitrogen fixation under water-deficit field conditions by a stress-tolerant alfalfa microsymbiont and its complete genome sequence : Short communication
Jozefkowicz, Cintia; Brambilla, Silvina Maricel; Frare, Romina Alejandra; Stritzler, Margarita; Piccinetti, Carlos Fabian; Puente, Mariana Laura; Berini, Carolina Andrea; Reyes Perez, Pedro Jose; Soto, Gabriela Cinthia; Ayub, Nicolás Daniel (2017-12)We here characterized the stress-tolerant alfalfa microsymbiont Sinorhizobium meliloti B401. B401-treated plants showed high nitrogen fixation rates under humid and semiarid environments. The production of glycine betaine ... -
Synthetic multi-antibiotic resistant plasmids in plant-associated bacteria from agricultural soils
Brambilla, Silvina Maricel; Frare, Romina Alejandra; Stritzler, Margarita; Soto, Gabriela Cynthia; Berini, Carolina Andrea; Jozefkowicz, Cintia; Ayub, Nicolás Daniel (Elsevier, 2020-09)Objectives: Unlike higher organisms such as domestic animals and cultivated plants, which display a robust reproductive isolation and limited dispersal ability, microbes exhibit an extremely promiscuous gene flow and can ...
