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Aportes a la producción sustentable de soja mediante el mejoramiento genético de los rizobios
Liebrenz, Karen Ivana (Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, 2022-07-14)La soja es el cultivo que consume más inoculantes a nivel global. Estos inoculantes están basados casi en su totalidad en cepas relacionadas de las especies Bradyrhizobium japonicum y Bradyrhizobium diazoefficiens. ... -
Engineering a commercial soybean inoculant to efficiently degrade glyphosate
Brambilla, Silvina Maricel; Pascuan, Cecilia Gabriela; Frare, Romina Alejandra; Liebrenz, Karen Ivana; Pesquero, Natalia; Ruiz, María Juana; Soto, Gabriela Cynthia; Cristos, Diego Sebastian; Ayub, Nicolás Daniel (Elsevier, 2025-06)Nitrogen-fixing rhizobia possess the well-characterized phn operon, which encodes the C–P lyase enzymatic complex responsible for degrading phosphonates such as glyphosate. However, glyphosate exerts a bacteriostatic effect ... -
Exploring the cooperation between nitrogen-fixing and non-fixing alfalfa rhizobia
Brambilla, Silvina Maricel; Liebrenz, Karen Ivana; Odorizzi, Ariel; Arolfo, Valeria; Maguire, Vanina Giselle; Moreno, Maria Valeria; Ruiz, Oscar Adolfo; Stritzler, Margarita; Serantes, Maria Laura; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Springer, 2024-07-05)Alfalfa plays a crucial role in agriculture due to its nitrogen-fixing partnership with rhizobia, resulting in nutritious forage. While the potential parasitic action of inefficient rhizobia has been extensively studied, ... -
Genomic and physiological plasticity in natural variants of commercial soybean inoculants supports the non-GMO status of base-edited inoculants
Cardillo, Maria Eugenia; Brambilla, Silvina Maricel; Liebrenz, Karen Ivana; Frare, Romina Alejandra; Maguire, Vanina Giselle; Soto, Gabriela Cynthia; Ruiz, Oscar Adolfo; Ayub, Nicolás Daniel (Springer, 2025-04)The regulatory classification of genome-edited microorganisms remains a topic of debate, particularly in the agricultural sector. In this study, we analyze the genomic and physiological differences of commercial variants ... -
Multiple ways to evade the bacteriostatic action of glyphosate in rhizobia include the mutation of the conserved serine 90 of the nitrogenase subunit NifH to alanine
Liebrenz, Karen Ivana; Frare, Romina Alejandra; Gomez, Maria Cristina; Pascuan, Cecilia Gabriela; Brambilla, Silvina Maricel; Soldini, Diego Omar; Maguire, Vanina; Carrio, Alejandro Javier; Ruiz, Oscar; McCormick, Wayne; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Elsevier, 2022-07)The genome resequencing of spontaneous glyphosate-resistant mutants derived from the soybean inoculant E109 allowed identifying genes most likely associated with the uptake (gltL and cya) and metabolism (zigA and betA) of ... -
Obtención de bioinoculantes tolerantes a herbicidas para incrementar la productividad y mejorar la inocuidad ambiental de los cultivos de soja
Liebrenz, Karen Ivana; Brambilla, Silvina Maricel; Gomez, Maria Cristina; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (INTA, 2022-11)El glifosato inhibe una enzima involucrada en la síntesis de los aminoácidos aromáticos afectando la funcionalidad de los rizobios endosimbiontes de soja, disminuyendo la fijación de nitrógeno la productividad de los ... -
Oligopeptide uptake improves oxidative stress resistance and nodulation competitiveness in soybean rhizobia
Brambilla, Silvina Maricel; Liebrenz, Karen Ivana; Frare, Romina Alejandra; Gomez, Maria Cristina; Stritzler, Margarita; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Elsevier, 2023-12)We here explored the mechanisms associated with oxidative stress resistance and root colonization by soybean rhizobia. Genomic and physiological analyses supported the fact that the mutation of a highly conserved valine ... -
Whole-Genome Resequencing of Spontaneous Oxidative Stress-Resistant Mutants Reveals an Antioxidant System of Bradyrhizobium japonicum Involved in Soybean Colonization
Liebrenz, Karen Ivana; Gomez, Maria Cristina; Brambilla, Silvina Maricel; Frare, Romina Alejandra; Stritzler, Margarita; Maguire, Vanina; Ruiz, Oscar; Soldini, Diego Omar; Pascuan, Cecilia Gabriela; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Springer, 2021-11)Soybean is the most inoculant-consuming crop in the world, carrying strains belonging to the extremely related species Bradyrhizobium japonicum and Bradyrhizobium diazoefficiens. Currently, it is well known that B. japonicum ...
