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Stearoyl-ACP desaturases (SADs) and fatty acid desaturases (FADs) play a critical role in plant lipid metabolism and also afect oil fatty acid composition introducing double bonds into the hydrocarbon chains to produce unsaturated fatty acids. In the present study, the genomic sequences of three SAD and three FAD candidate genes were characterized in olive and their expression was evaluated in diferent plant tissues. OeSAD genes corresponded to olive SAD1
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dc.contributor.author | Contreras Valentín, Ana Cibeles | |
dc.contributor.author | Mariotti, Roberto | |
dc.contributor.author | Mousavi, S. | |
dc.contributor.author | Baldoni, L. | |
dc.contributor.author | Guerrero, C. | |
dc.contributor.author | Roka, L. | |
dc.contributor.author | Cultrera, N. | |
dc.contributor.author | Pierantozzi, Pierluigi | |
dc.contributor.author | Maestri, Damián | |
dc.contributor.author | Gentili, Luciana | |
dc.contributor.author | Tivani, Martín | |
dc.contributor.author | Torres, Myriam Mariela | |
dc.date.accessioned | 2021-05-05T18:56:59Z | |
dc.date.available | 2021-05-05T18:56:59Z | |
dc.date.issued | 2020-05-28 | |
dc.identifier.issn | 0301-4851 | |
dc.identifier.issn | 1573-4978 | |
dc.identifier.other | https://doi.org/10.1007/s11033-020-05554-9 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/9283 | |
dc.identifier.uri | https://link.springer.com/article/10.1007/s11033-020-05554-9 | |
dc.description.abstract | Stearoyl-ACP desaturases (SADs) and fatty acid desaturases (FADs) play a critical role in plant lipid metabolism and also afect oil fatty acid composition introducing double bonds into the hydrocarbon chains to produce unsaturated fatty acids. In the present study, the genomic sequences of three SAD and three FAD candidate genes were characterized in olive and their expression was evaluated in diferent plant tissues. OeSAD genes corresponded to olive SAD1 and SAD2 and to a newly identifed OeSAD4, sharing the conserved protein structure with other plant species. On the other hand, the full-length genomic sequences of two microsomal OeFAD genes (FAD2-1 and FAD2-2) and the plastidial FAD6, were released. When the level of expression was tested on diferent tissues of cv. Leccino, OeSAD1 and OeSAD2 were mainly expressed in the fruits, while OeFAD genes showed the lowest expression in this tissue. The mRNA profling of all genes was directly studied in fruits of Leccino and Coratina cultivars during fruit development. In both genotypes, the expression level of OeSAD1and OeSAD2 had the highest value during and after the pit-hardening period, when oil accumulation in fruit mesocarp is intensively increasing. Furthermore, the expression level of both OeFAD2 genes, which were the main candidates for oleic acid desaturation, were almost negligible during fruit ripening. These results have made possible to defne candidate genes of the machinery regulation of fatty acid composition in olive oil, providing information on their sequence, gene structure and chromosomal location. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | Springer | es_AR |
dc.rights | info:eu-repo/semantics/restrictedAccess | es_AR |
dc.source | Molecular Biology Reports 47 (6) : 4345-4355 (May 2020) | es_AR |
dc.subject | Olea europaea | es_AR |
dc.subject | Expresión Génica | es_AR |
dc.subject | Mesocarpio | es_AR |
dc.subject | Gene Expression | eng |
dc.subject | Mesocarp | eng |
dc.subject | Genética | |
dc.subject | Genetics | eng |
dc.subject.other | Olivo | es_AR |
dc.title | Characterization and validation of olive FAD and SAD gene families: expression analysis in diferent tissues and during fruit development | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.description.origen | EEA San Juan | es_AR |
dc.description.fil | Fil: Contreras Valetín, Ana Cibeles. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina. | es_AR |
dc.description.fil | Fil: Contreras Valetín, Ana Cibeles. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Mariotti, Roberto. Consiglio Nazionale delle Ricerche. Institute of Biosciences and Bioresources; Italia | es_AR |
dc.description.fil | Fil: Mousavi, S. Consiglio Nazionale delle Ricerche. Institute of Biosciences and Bioresources; Italia | es_AR |
dc.description.fil | Fil: Baldoni, L. Consiglio Nazionale delle Ricerche. Institute of Biosciences and Bioresources; Italia | es_AR |
dc.description.fil | Fil: Guerrero, C. University of Malaga. Science Faculty. Department of Molecular Biology and Biochemistry ; España | es_AR |
dc.description.fil | Fil: Roka, L. Agricultural University of Athens. Department of Biotechnology; Grecia | es_AR |
dc.description.fil | Fil: Cultrera, N. Consiglio Nazionale delle Ricerche. Institute of Biosciences and Bioresources; Italia | es_AR |
dc.description.fil | Fil: Pierantozzi, Pierluigi. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina. | es_AR |
dc.description.fil | Fil: Pierantozzi, Pierluigi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Maestri, Damián. Universidad Nacional de Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina | es_AR |
dc.description.fil | Fil: Maestri, Damián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Gentili, Luciana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina. | es_AR |
dc.description.fil | Fil: Gentili, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Tivani, Martín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina. | es_AR |
dc.description.fil | Fil: Tivani, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Torres, Myriam Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina. | es_AR |
dc.description.fil | Fil: Torres, Myriam Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.subtype | cientifico |
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