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Abstract
Citrus psorosis virus (CPsV) is the causal agent of psorosis, an important disease of citrus. Sanitary and certification programmes helped reduce disease damage caused by psorosis and other graft‐transmissible diseases in many citrus‐growing regions. For quarantine and certification programmes, most of these diseases are currently diagnosed using biological indexing (BI) on sensitive indicator plants. In the case of citrus psorosis, CPsV can be detected [ver mas...]
dc.contributor.authorSimeone, Melina
dc.contributor.authorGomez, Claudio Andres
dc.contributor.authorBertalmío, Ana
dc.contributor.authorRuiz, Esperanza
dc.contributor.authorHauteville, Claudia
dc.contributor.authorGodoy Suarez, Laura Magdalena
dc.contributor.authorTito, Blas Alfredo
dc.contributor.authorGarcía, María Laura
dc.date.accessioned2021-03-08T16:24:01Z
dc.date.available2021-03-08T16:24:01Z
dc.date.issued2021-01
dc.identifier.issn1365-3059
dc.identifier.otherhttps://doi.org/10.1111/ppa.13341
dc.identifier.urihttp://hdl.handle.net/20.500.12123/8837
dc.identifier.urihttps://bsppjournals.onlinelibrary.wiley.com/doi/abs/10.1111/ppa.13341
dc.description.abstractCitrus psorosis virus (CPsV) is the causal agent of psorosis, an important disease of citrus. Sanitary and certification programmes helped reduce disease damage caused by psorosis and other graft‐transmissible diseases in many citrus‐growing regions. For quarantine and certification programmes, most of these diseases are currently diagnosed using biological indexing (BI) on sensitive indicator plants. In the case of citrus psorosis, CPsV can be detected by molecular methods such as quantitative reverse transcription PCR (RT‐qPCR), which is cheaper and faster than BI, but sensitivity, reliability, and reproducibility of both procedures have not been compared so far. In this work, 128 plants from Argentina and Uruguay were analysed using BI and CPsV detection by the RT‐qPCR assay. Almost perfect agreement between both diagnostic procedures and sensitivity, specificity, and estimated likelihood ratios indicate that RT‐qPCR is equivalent to BI for citrus psorosis diagnosis, thus providing confidence in the quick diagnostic procedure to monitor the sanitary status of citrus trees.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherWileyes_AR
dc.relationinfo:eu-repograntAgreement/INTA/ERIOS-630081/AR./Incremento en la calidad, cantidad y diversidad de la producción frutícola de Entre Ríoses_AR
dc.relationinfo:eu-repograntAgreement/INTA/2019-PD-E4-I081-001/2019-PD-E4-I081-001/AR./Generación de reactivos, desarrollo de metodologías, validación y acreditación de ensayos para el diagnóstico de patógenos vegetales.es_AR
dc.relationinfo:eu-repograntAgreement/INTA/2019-RIST-E4-I091-001/2019-RIST-E4-I091-001/AR./Multiplicación de propágulos de sanidad controladaes_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourcePlant Pathology (First published: 18 January 2021)es_AR
dc.subjectCitruses_AR
dc.subjectEnfermedades de las Plantases_AR
dc.subjectPlant Diseaseseng
dc.subjectDiagnósticoes_AR
dc.subjectDiagnosiseng
dc.subjectPCRes_AR
dc.subjectVirus de las Plantases_AR
dc.subjectPlant Viruseseng
dc.subject.otherVirus de la Psoriasis de los Cítricoses_AR
dc.subject.otherCitrus Psorosis Viruseng
dc.titleDetection of citrus psorosis virus by RT‐qPCR validated by diagnostic parameterses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Concordiaes_AR
dc.description.filFil: Simeone, M. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentinaes_AR
dc.description.filFil: Gomez, Claudio Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentinaes_AR
dc.description.filFil: Bertalmío, Ana. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental Salto Grande; Uruguayes_AR
dc.description.filFil: Ruiz, Esperanza. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Bioactivos; Argentinaes_AR
dc.description.filFil: Hauteville, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentinaes_AR
dc.description.filFil: Godoy Suarez, Laura Magdalena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentinaes_AR
dc.description.filFil: Tito, Blas Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentinaes_AR
dc.description.filFil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentinaes_AR
dc.subtypecientifico


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