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Abstract
Foot-and-Mouth Disease (FMD) is an acute viral disease that causes important economy losses. Vaccines with new low-cost adjuvants that stimulate protective immune responses are needed and can be assayed in a mouse model to predict their effectiveness in cattle. Immunostimulant Particle Adjuvant (ISPA), also known as cage-like particle adjuvant, consisting of lipid boxes of dipalmitoyl-phosphatidylcholine, cholesterol, sterylamine, alpha-tocopherol, and [ver mas...]
dc.contributor.authorBidart, Juan Esteban
dc.contributor.authorKornuta, Claudia Alejandra
dc.contributor.authorGammella, Mariela
dc.contributor.authorGnazzo, Victoria
dc.contributor.authorSoria, Ivana
dc.contributor.authorLangellotti, Cecilia Ana
dc.contributor.authorMongini, Claudia
dc.contributor.authorGalarza, Roxana Ivon
dc.contributor.authorCalvinho, Luis Fernando
dc.contributor.authorLupi, Giuliana
dc.contributor.authorQuattrocchi, Valeria
dc.contributor.authorMarcipar, Ivan
dc.contributor.authorZamorano, Patricia Ines
dc.date.accessioned2021-02-19T11:25:37Z
dc.date.available2021-02-19T11:25:37Z
dc.date.issued2020-07
dc.identifier.issn2297-1769
dc.identifier.otherhttps://doi.org/10.3389/fvets.2020.00396
dc.identifier.urihttp://hdl.handle.net/20.500.12123/8692
dc.identifier.urihttps://www.frontiersin.org/articles/10.3389/fvets.2020.00396/full
dc.description.abstractFoot-and-Mouth Disease (FMD) is an acute viral disease that causes important economy losses. Vaccines with new low-cost adjuvants that stimulate protective immune responses are needed and can be assayed in a mouse model to predict their effectiveness in cattle. Immunostimulant Particle Adjuvant (ISPA), also known as cage-like particle adjuvant, consisting of lipid boxes of dipalmitoyl-phosphatidylcholine, cholesterol, sterylamine, alpha-tocopherol, and QuilA saponin, was shown to enhance protection of a recombinant vaccine against Trypanosoma cruzi in a mouse model. Thus, in the present work, we studied the effects on the magnitude and type of immunity elicited in mice and cattle in response to a vaccine based on inactivated FMD virus (iFMDV) formulated with ISPA. It was demonstrated that iFMDV–ISPA induced protection in mice against challenge and elicited a specific antibody response in sera, characterized by a balanced Th1/Th2 profile. In cattle, the antibody titers reached corresponded to an expected percentage of protection (EPP) higher than 80%. EPP calculates the probability that livestock would be protected against a 10,000 bovine infectious doses challenge after vaccination. Moreover, in comparison with the non-adjuvanted iFMDV vaccine, iFMDV–ISPA elicited an increased specific T-cell response against the virus, including higher interferon gamma (IFNγ)+/CD8+ lymphocyte production in cattle. In this work, we report for first time that an inactivated FMDV serotype A vaccine adjuvanted with ISPA is capable of inducing protection against challenge in a murine model and of improving the specific immune responses against the virus in cattle.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherFrontiers Mediaes_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceFrontiers in Veterinary Science 7 : 396 (Julio 2020)es_AR
dc.subjectFoot and Mouth Diseaseeng
dc.subjectFiebre Aftosaes_AR
dc.subjectAphthoviruseng
dc.subjectVirus Fiebre Aftosaes_AR
dc.subjectImmunostimulantseng
dc.subjectInmunoestimulanteses_AR
dc.subjectVaccine Adjuvantseng
dc.subjectCoadyuvantes de Vacunases_AR
dc.subjectAdjuvantseng
dc.subjectCoadyuvanteses_AR
dc.subjectProtectioneng
dc.subjectProtecciónes_AR
dc.subjectImmune Responseeng
dc.subjectRespuesta Inmunológicaes_AR
dc.subjectCattleeng
dc.subjectGanado Bovinoes_AR
dc.subject.otherMouse Modeleng
dc.subject.otherModelo de Ratónes_AR
dc.titleA new cage-like particle adjuvant enhances protection of foot-and-mouth disease vaccinees_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Virologíaes_AR
dc.description.filFil: Bidart, Juan Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Kornuta, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Gammella, Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Gnazzo, Victoria. Instituto Nacional de Medicina Tropical; Argentinaes_AR
dc.description.filFil: Soria, Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentinaes_AR
dc.description.filFil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Mongini, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Galarza, Roxana Ivon. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cuenca del Salado. Agencia de Extensión Rural Chascomús; Argentinaes_AR
dc.description.filFil: Calvinho, Luis Fernando. INTA. Estación Experimental Agropecuaria Rafaela; Argentinaes_AR
dc.description.filFil: Lupi, Giuliana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Marcipar, Ivan Sergio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Tecnología Inmunológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad del Salvador; Argentinaes_AR
dc.subtypecientifico


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