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Abstract
New technologies in the field of vaccinology arise as a necessity for the treatment and control of many diseases. Whole virus inactivated vaccines and modified live virus ones used against Bovine Herpesvirus‐1 (BoHV‐1) infection have several disadvantages. Previous works on DNA vaccines against BoHV‐1 have demonstrated the capability to induce humoral and cellular immune responses. Nevertheless, ‘naked’ DNA induces low immunogenic response. Thus, loading [ver mas...]
dc.contributor.authorKornuta, Claudia Alejandra
dc.contributor.authorBidart, , Juan Esteban
dc.contributor.authorSoria, Ivana
dc.contributor.authorGammella, Mariela
dc.contributor.authorQuattrocchi, Valeria
dc.contributor.authorPappalardo, Juan Sebastián
dc.contributor.authorSalmaso, Stefano
dc.contributor.authorTorchilin, Vladimir P.
dc.contributor.authorCheuquepan Valenzuel, Felipe Andres
dc.contributor.authorHecker, Yanina
dc.contributor.authorMoore, Dadin P.
dc.contributor.authorZamorano, Patricia Ines
dc.contributor.authorLangellotti, Cecilia Ana
dc.date.accessioned2021-01-15T13:48:31Z
dc.date.available2021-01-15T13:48:31Z
dc.date.issued2020-07
dc.identifier.issn1865-1674
dc.identifier.otherhttps://doi.org/10.1111/tbed.13718
dc.identifier.urihttp://hdl.handle.net/20.500.12123/8603
dc.description.abstractNew technologies in the field of vaccinology arise as a necessity for the treatment and control of many diseases. Whole virus inactivated vaccines and modified live virus ones used against Bovine Herpesvirus‐1 (BoHV‐1) infection have several disadvantages. Previous works on DNA vaccines against BoHV‐1 have demonstrated the capability to induce humoral and cellular immune responses. Nevertheless, ‘naked’ DNA induces low immunogenic response. Thus, loading of antigen encoding DNA sequences in liposomal formulations targeting dendritic cell receptors could be a promising strategy to better activate these antigen‐presenting cells (APC). In this work, a DNA‐based vaccine encoding the truncated version of BoHV‐1 glycoprotein D (pCIgD) was evaluated alone and encapsulated in a liposomal formulation containing LPS and decorated with MANα1‐2MAN‐PEG‐DOPE (pCIgD‐Man‐L). The vaccinations were performed in mice and bovines. The results showed that the use of pCIgD‐Man‐L enhanced the immune response in both animal models. For humoral immunity, significant differences were achieved when total antibody titres and isotypes were assayed in sera. Regarding cellular immunity, a significant increase in the proliferative response against BoHV‐1 was detected in animals vaccinated with pCIgD‐Man‐L when compared to the response induced in animals vaccinated with pCIgD. In addition, upregulation of CD40 molecules on the surface of bovine dendritic cells (DCs) was observed when cells were stimulated and activated with the vaccine formulations. When viral challenge was performed, bovines vaccinated with MANα1‐2MAN‐PEG‐DOPE elicited better protection which was evidenced by a lower viral excretion. These results demonstrate that the dendritic cell targeting using MANα1‐2MAN decorated liposomes can boost the immunogenicity resulting in a long‐lasting immunity. Liposomes decorated with MANα1‐2MAN‐PEG‐DOPE were tested for the first time as a DNA vaccine nanovehicle in cattle as a preventive treatment against BoHV‐1. These results open new perspectives for the design of vaccines for the control of bovine rhinotracheitis.es_AR
dc.formatapplication/pdfeng
dc.language.isoenges_AR
dc.publisherWileyeng
dc.relationinfo:eu-repograntAgreement/INTA/PNBIO-1131032/AR./Desarrollo de herramientas biotecnológicas para la prevención y el control de enfermedades pecuarias: vacunas, diagnóstico y eIdemiología molecular.es_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceTransboundary and emerging diseases (Julio 2020)es_AR
dc.subjectLiposomes (Organelles)es_AR
dc.subjectLiposomas (Organulos)es_AR
dc.subjectImmunogeneticseng
dc.subjectInmunogenéticaes_AR
dc.subjectVaccineseng
dc.subjectVacunaes_AR
dc.subjectHerpesviridaees_AR
dc.titleMANα1-2MAN decorated liposomes enhance the immunogenicity induced by a DNA vaccine against BoHV-1es_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Virologíaes_AR
dc.description.filFil: Kornuta, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentinaes_AR
dc.description.filFil: Bidart, Juan Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentinaes_AR
dc.description.filFil: Soria, Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentinaes_AR
dc.description.filFil: Gammella, Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentinaes_AR
dc.description.filFil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentinaes_AR
dc.description.filFil: Pappalardo, Juan Sebastián. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentinaes_AR
dc.description.filFil: Salmaso, Stefano. Universita degli Studi di Padova. Department of Pharmaceutical and Pharmacological Sciences; Italiaes_AR
dc.description.filFil: Torchilin, Vladimir P. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidoses_AR
dc.description.filFil: Cheuquepán Valenzuela, Felipe Andrés. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce); Argentina.es_AR
dc.description.filFil: Hecker, Yanina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentinaes_AR
dc.description.filFil: Moore, Dadin P. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentinaes_AR
dc.description.filFil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentinaes_AR
dc.description.filFil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Kornuta, Claudia Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
dc.description.filFil: Bidart, Juan Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
dc.description.filFil: Soria, Ivana. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); Argentina
dc.description.filFil: Gammella, Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
dc.description.filFil: Quattrocchi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
dc.description.filFil: Cheuquepán Valenzuela, Felipe Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
dc.description.filFil: Cheuquepán Valenzuela, Felipe Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
dc.description.filFil: Zamorano, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
dc.description.filFil: Zamorano, Patricia Ines. Universidad del Salvador; Argentina
dc.description.filFil: Hecker, Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
dc.subtypecientifico


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