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Resumen
Interferon lambda (IFN-λ) plays an important role in inducing an antiviral state in mucosal surfaces and has been used as an effective biotherapeutic against several viral diseases. Here we performed a proof of concept study on the activity of a biologically active recombinant bovine IFN-λ (rIFN-λ) produced in eukaryotic cells against Bovine Viral Diarrhea Virus (BVDV) in cattle. We first confirmed the lack of toxicity of different concentrations of [ver mas...]
dc.contributor.authorQuintana, Maria Eugenia
dc.contributor.authorCardoso, Nancy
dc.contributor.authorPereyra, Walter Rodrigo
dc.contributor.authorBarone, Lucas Jose
dc.contributor.authorBarrionuevo, Florencia Mariel
dc.contributor.authorMansilla, Florencia Celeste
dc.contributor.authorTurco, Cecilia Soledad
dc.contributor.authorCapozzo, Alejandra
dc.date.accessioned2021-01-08T16:14:54Z
dc.date.available2021-01-08T16:14:54Z
dc.date.issued2020-12
dc.identifier.issn0165-2427
dc.identifier.otherhttps://doi.org/10.1016/j.vetimm.2020.110145
dc.identifier.urihttp://hdl.handle.net/20.500.12123/8582
dc.identifier.urihttps://www.sciencedirect.com/science/article/abs/pii/S0165242720301719
dc.description.abstractInterferon lambda (IFN-λ) plays an important role in inducing an antiviral state in mucosal surfaces and has been used as an effective biotherapeutic against several viral diseases. Here we performed a proof of concept study on the activity of a biologically active recombinant bovine IFN-λ (rIFN-λ) produced in eukaryotic cells against Bovine Viral Diarrhea Virus (BVDV) in cattle. We first confirmed the lack of toxicity of different concentrations of rIFN-λ in bovine peripheral blood cells and the safety of its subcutaneous application in calves in doses up to 12 IU/kg. The antiviral activity of the rIFN-λ against BVDV was assessed in calves that were inoculated with 6 IU/kg of rIFN-λ (n = 4) or mock-treated (n = 2) two days before and after challenge with a BVDV type-2 non-cytopathic strain. Mock-treated animals developed respiratory disease, shedded the virus from 4 to 7 days post-infection (dpi) and had viremia between 4 and 14 dpi. Conversely, calves treated with rIFN-λ did not develop clinical symptoms. The virus was not found in nasal secretions or sera. Only one animal had a positive viral RNA detection in serum at 7 dpi. All infected animals treated with rIFN-λ increased systemic type-I IFNs levels at 4 dpi. The antiviral treatment induced an earlier onset of the anti-BVDV neutralizing antibodies. Altogether, these results constitute the proof-of-principle of bovine IFN-λ as an antiviral biotherapeutic to protect cattle against the clinical disease caused by BVDV.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceVeterinary Immunology and Immunopathology 230 : 110145 (Diciembre 2020)es_AR
dc.subjectInterferonseng
dc.subjectInterferonases_AR
dc.subjectBovine Viral Diarrhoeaeng
dc.subjectDiarrea Viral Bovinaes_AR
dc.subjectDisease Controleng
dc.subjectControl de Enfermedadeses_AR
dc.subjectAntiviral Agentseng
dc.subjectViricidases_AR
dc.subjectCattleeng
dc.subjectGanado Bovinoes_AR
dc.titleInterferon lambda protects cattle against bovine viral diarrhea virus infectiones_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Virologíaes_AR
dc.description.filFil: Quintana, María Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentinaes_AR
dc.description.filFil: Cardoso, Nancy Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentinaes_AR
dc.description.filFil: Pereyra Walter Rodrigo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentinaes_AR
dc.description.filFil: Barone, Lucas Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentinaes_AR
dc.description.filFil: Barrionuevo, Florencia Mariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentinaes_AR
dc.description.filFil: Mansilla, Florencia Celeste. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentinaes_AR
dc.description.filFil: Turco Cecilia Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentinaes_AR
dc.description.filFil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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