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Abstract
Bovine herpesvirus 1 (BoHV-1) causes several clinical syndromes in cattle worldwide. There are 3 subtypes of BoHV-1: 1.1, 1.2a, and 1.2b. Several molecular methods are commonly used in the detection and characterization of BoHV-1. Among them, restriction endonuclease analysis (REA) and single-nucleotide polymorphism (SNP) analysis of the complete viral genome allow classification of BoHV-1 into different subtypes. However, developing countries need [ver mas...]
dc.contributor.authorMaidana, Silvina Soledad
dc.contributor.authorMiño, Samuel
dc.contributor.authorApostolo, Romina Maria
dc.contributor.authorDe Stefano, Gabriel Alejandro
dc.contributor.authorRomera, Sonia Alejandra
dc.date.accessioned2020-09-10T16:40:56Z
dc.date.available2020-09-10T16:40:56Z
dc.date.issued2020-02
dc.identifier.issn1943-4936
dc.identifier.issn1040-6387
dc.identifier.otherhttps://doi.org/10.1177/1040638719898692
dc.identifier.urihttp://hdl.handle.net/20.500.12123/7868
dc.identifier.urihttps://journals.sagepub.com/doi/abs/10.1177/1040638719898692
dc.description.abstractBovine herpesvirus 1 (BoHV-1) causes several clinical syndromes in cattle worldwide. There are 3 subtypes of BoHV-1: 1.1, 1.2a, and 1.2b. Several molecular methods are commonly used in the detection and characterization of BoHV-1. Among them, restriction endonuclease analysis (REA) and single-nucleotide polymorphism (SNP) analysis of the complete viral genome allow classification of BoHV-1 into different subtypes. However, developing countries need simpler and cheaper screening assays for routine testing. We designed a standard multiplex PCR followed by a REA assay allowing straightforward subclassification of all BoHV-1 isolates tested into 1.1, 1.2a, and 1.2b subtypes based on the analysis of fragment length polymorphism. Our standard multiplex PCR-REA was used to analyze 33 field strains of BoHV-1 isolated from various tissues. The assay confirmed the subtype identified previously by REA. In addition, non-polymorphic or undigested fragments were sequenced in order to confirm the mutation affecting the RE HindIII site. Our PCR-REA method is an affordable and rapid test that will subtype all BoHV-1 strains.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherSage Publicationses_AR
dc.relationinfo:eu-repograntAgreement/INTA/PNSA/1115054/AR./Enfermedades parasitarias, infecciosas y tóxico metabólicas que afectan la productividad de los bóvidos para producción de carne y leche.es_AR
dc.relationinfo:eu-repograntAgreement/INTA/PNSA/1115055/AR./Enfermedades infecciosas, parasitarias y toxico metabólicas que afectan la productividad de los ovinos, caprinos y camélidos.es_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceJournal of Veterinary Diagnostic Investigation 32 (1) : 112-117 (Febrero 2020)es_AR
dc.subjectBovine Herpesviruseng
dc.subjectHerpes Virus Bovinoes_AR
dc.subjectPCRes_AR
dc.subjectGenetic Techniqueseng
dc.subjectTécnica Genéticaes_AR
dc.subjectGenetic Isolationeng
dc.subjectAislamiento Genéticoes_AR
dc.subject.otherBoHV-1es_AR
dc.subject.otherMultiplex PCReng
dc.subject.otherPCR Múltiplees_AR
dc.subject.otherNew Molecular Methodeng
dc.subject.otherNuevo Método Moleculares_AR
dc.titleA new molecular method for the rapid subtyping of bovine herpesvirus 1 field isolateses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/acceptedVersiones_AR
dc.description.origenInstituto de Virologíaes_AR
dc.description.filFil: Maidana, Silvina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Apostolo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Esquel. Grupo de Producción Animal; Argentinaes_AR
dc.description.filFil: De Stefano, Gabriel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentinaes_AR
dc.description.filFil: Romera, Sonia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad del Salvador. Cátedra de Inmunología; Argentina. Universidad de Morón. Facultad de Ciencias exactas, Químicas y Naturales. Cátedra de Inmunogenética; Argentinaes_AR
dc.subtypecientifico


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