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Background: Symptoms of grapevine leafroll disease (GLRD) in red-fruited wine grape (Vitis vinifera L.) cultivars consist of green veins and red and reddish-purple discoloration of inter-veinal areas of leaves. The reddish-purple color of symptomatic leaves may be due to the accumulation of anthocyanins and could reflect an up-regulation of genes involved in their biosynthesis.
Results: We examined six putative constitutively expressed genes, Ubiquitin,
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dc.contributor.author | Gutha, Linga R. | |
dc.contributor.author | Casassa, Luis Federico | |
dc.contributor.author | Harbertson, James F. | |
dc.contributor.author | Naidu, Rayapati A. | |
dc.date.accessioned | 2020-07-13T12:50:16Z | |
dc.date.available | 2020-07-13T12:50:16Z | |
dc.date.issued | 2010-08 | |
dc.identifier.issn | 1471-2229 | |
dc.identifier.other | https://doi.org/10.1186/1471-2229-10-187 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/7541 | |
dc.identifier.uri | https://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-10-187 | |
dc.description.abstract | Background: Symptoms of grapevine leafroll disease (GLRD) in red-fruited wine grape (Vitis vinifera L.) cultivars consist of green veins and red and reddish-purple discoloration of inter-veinal areas of leaves. The reddish-purple color of symptomatic leaves may be due to the accumulation of anthocyanins and could reflect an up-regulation of genes involved in their biosynthesis. Results: We examined six putative constitutively expressed genes, Ubiquitin, Actin, GAPDH, EF1-a, SAND and NAD5, for their potential as references for normalization of gene expression in reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR). Using the geNorm program, a combination of two genes (Actin and NAD5) was identified as the stable set of reference genes for normalization of gene expression data obtained from grapevine leaves. By using gene-specific RT-qPCR in combination with a reliable normalization factor, we compared relative expression of the flavonoid biosynthetic pathway genes between leaves infected with Grapevine leafroll-associated virus 3 (GLRaV-3) and exhibiting GLRD symptoms and virus-free green leaves obtained from a red-fruited wine grape cultivar (cv. Merlot). The expression levels of these different genes ranged from two- to fifty-fold increase in virus-infected leaves. Among them, CHS3, F3'5'H, F3H1, LDOX, LAR1 and MybA1 showed greater than 10-fold increase suggesting that they were expressed at significantly higher levels in virus-infected symptomatic leaves. HPLC profiling of anthocyanins extracted from leaves indicated the presence of cyanidin-3-glucoside and malvidin-3-glucoside only in virus-infected symptomatic leaves. The results also showed 24% higher levels of flavonols in virus-infected symptomatic leaves than in virus-free green leaves, with quercetin followed by myricetin being the predominant compounds. Proanthocyanidins, estimated as total tannins by protein precipitation method, were 36% higher in virus-infected symptomatic leaves when compared to virus-free green leaves. Conclusions: The results, the first example to our knowledge, showed that modulation of the flavonoid biosynthetic pathway occurred in GLRaV-3-infected leaves of a red-fruited wine grape cultivar (cv. Merlot) leading to de novo synthesis of two classes of anthocyanins. These anthocyanins have contributed to the expression of reddish-purple color of virus-infected grapevine leaves exhibiting GLRD symptoms. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | BMC | es_AR |
dc.rights | info:eu-repo/semantics/openAccess | es_AR |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
dc.source | BMC Plant Biology 10 : article number: 187 (2010) | es_AR |
dc.subject | Vid | es_AR |
dc.subject | Grapevines | eng |
dc.subject | Virus de las Plantas | es_AR |
dc.subject | Plant Viruses | eng |
dc.subject | Flavonoides | es_AR |
dc.subject | Flavonoids | eng |
dc.subject | Antocianinas | es_AR |
dc.subject | Anthocyanins | eng |
dc.subject | Genética | es_AR |
dc.subject | Genetics | eng |
dc.title | Modulation of flavonoid biosynthetic pathway genes and anthocyanins due to virus infection in grapevine (Vitis viniferaL.) leaves | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
dc.description.origen | EEA Mendoza | es_AR |
dc.description.fil | Fil: Gutha, Linga R. Washington State University. Irrigated Agriculture Research and Extension Center. Department of Plant Pathology; Estados Unidos | es_AR |
dc.description.fil | Fil: Casassa, Luis Federico. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Centro de Estudios de Enología; Argentina. Washington State University. Irrigated Agriculture Research and Extension Center. School of Food Science; Estados Unidos | es_AR |
dc.description.fil | Fil: Harbertson, James F. Washington State University. Irrigated Agriculture Research and Extension Center. School of Food Science; Estados Unidos | es_AR |
dc.description.fil | Fil: Naidu, Rayapati A. Washington State University. Irrigated Agriculture Research and Extension Center. Department of Plant Pathology; Estados Unidos | es_AR |
dc.subtype | cientifico |
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