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Quantification of endogenous hormones in plants is essential to understand their growth, development and response to biotic and abiotic stresses. However, it is challenging to develop high-throughput sample treatments from complex plant tissues containing low amounts of structurally unrelated and labile phytohormones while delivering clean and analyte-enriched extracts. In this paper we propose the use of supramolecular solvents (SUPRASs) made up or
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dc.contributor.author | Yonny, Melisa Evangelina | |
dc.contributor.author | Ballesteros-Gómez, Ana | |
dc.contributor.author | Toscano Adamo, Maria L. | |
dc.contributor.author | Rodriguez Torressi, Ariel | |
dc.contributor.author | Nazareno, Mónica Azucena | |
dc.contributor.author | Rubio, Soledad | |
dc.date.accessioned | 2020-06-25T14:20:50Z | |
dc.date.available | 2020-06-25T14:20:50Z | |
dc.date.issued | 2020-11 | |
dc.identifier.issn | 0039-9140 | |
dc.identifier.other | https://doi.org/10.1016/j.talanta.2020.121249 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/7470 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/abs/pii/S0039914020305403 | |
dc.description.abstract | Quantification of endogenous hormones in plants is essential to understand their growth, development and response to biotic and abiotic stresses. However, it is challenging to develop high-throughput sample treatments from complex plant tissues containing low amounts of structurally unrelated and labile phytohormones while delivering clean and analyte-enriched extracts. In this paper we propose the use of supramolecular solvents (SUPRASs) made up or inverted hexagonal nanostructures of alkanols to address this challenge. The strategy was applied, as a proof of concept, to the quantification of stress-related phytohormones belonging to different categories (abscisic acid, salicylic acid, jasmonic acid, methyl jasmonate and 3-indoleacetic acid) in melon and pepper leaves. Sample treatment consisted in a single extraction-cleanup step involving the use of a low volume of SUPRAS (244 μL), the stirring (5 min) and centrifugation (15 min) of the sample at room temperature, and the direct analysis of the extract by liquid chromatography tandem mass spectrometry (LC-MS/MS). This high-throughput sample treatment method delivered excellent results for the target phytohormones regarding absolute recoveries (80–92%), method quantification limits (0.05–2 ng g−1), reproducibility (1–7%) and matrix effects (+13 to −31%), in both melon and pepper leaves, compared to reported methods based on repetitive solvent extraction, purification and solvent evaporation steps. The method was successfully applied to determine target hormones in melon and pepper plants for the evaluation of the effect of thermal stress. It was found that their concentration increased in the ranges 1.2–1.9 and 1.3–3.8 times in melon and pepper leaves, respectively, compared with control samples. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.rights | info:eu-repo/semantics/restrictedAccess | es_AR |
dc.source | Talanta Volume 219 : 121249 (November 2020) | es_AR |
dc.subject | Sustancias de Crecimiento Vegetal | es_AR |
dc.subject | Plant Growth Substances | eng |
dc.subject | Solventes | es_AR |
dc.subject | Solvents | eng |
dc.subject | Estrés Térmico | es_AR |
dc.subject | Heat Stress | eng |
dc.subject | Tejidos Vegetales | es_AR |
dc.subject | Plant Tissues | eng |
dc.subject.other | Fitohormonas | es_AR |
dc.subject.other | Phytohormones | eng |
dc.title | Supramolecular solvent-based high-throughput sample treatment for monitoring phytohormones in plant tissues | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.description.origen | EEA Santiago del Estero | es_AR |
dc.description.fil | Fil: Yonny, Melisa. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias. Laboratorio de Antioxidantes y Procesos Oxidativos; Argentina | es_AR |
dc.description.fil | Fil: Ballesteros-Gómez, Ana. Universidad de Córdoba. Departamento de Química Analítica. Instituto de Química Fina y Nanoquímica; España | es_AR |
dc.description.fil | Fil: Toscano Adamo, Maria L. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias. Laboratorio de Antioxidantes y Procesos Oxidativos; Argentina | es_AR |
dc.description.fil | Fil: Rodriguez Torressi, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero; Argentina | es_AR |
dc.description.fil | Fil: Nazareno, Mónica A. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias. Laboratorio de Antioxidantes y Procesos Oxidativos; Argentina | es_AR |
dc.description.fil | Fil: Rubio, Soledad. Universidad de Córdoba. Departamento de Química Analítica. Instituto de Química Fina y Nanoquímica; España | es_AR |
dc.subtype | cientifico |
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