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Abstract
Neospora caninum is a world-wide distributed apicomplexan parasite, causative agent of bovine neosporosis, which is one of the major causes of reproductive losses in cattle affecting both dairy and beef industries. Several techniques are regularly used for the diagnosis of bovine neosporosis. Amongst them, serological techniques are generally preferred, being IFAT the traditionally accepted reference method for diagnosis (gold standard). However, ELISA [ver mas...]
dc.contributor.authorMansilla, Florencia Celeste
dc.contributor.authorGual, Ignacio
dc.contributor.authorPereyra, Walter Rodrigo
dc.contributor.authorCardoso, Nancy Patricia
dc.contributor.authorTurco, Cecilia
dc.contributor.authorCapozzo, Alejandra Victoria
dc.date.accessioned2020-06-24T15:24:58Z
dc.date.available2020-06-24T15:24:58Z
dc.date.issued2019-08-26
dc.identifier.issn2348-9790
dc.identifier.urihttp://www.annexpublishers.com/articles/JVSAH/7204-Development-and-Validation-of-a-Novel-ELISA-for-the-Detection-of-Neospora-Caninum-Antibodies-in-Bovine-Sera.pdf
dc.identifier.urihttp://hdl.handle.net/20.500.12123/7466
dc.description.abstractNeospora caninum is a world-wide distributed apicomplexan parasite, causative agent of bovine neosporosis, which is one of the major causes of reproductive losses in cattle affecting both dairy and beef industries. Several techniques are regularly used for the diagnosis of bovine neosporosis. Amongst them, serological techniques are generally preferred, being IFAT the traditionally accepted reference method for diagnosis (gold standard). However, ELISA has become the most eligible method for large-scale screening of specific antibodies against N. caninum in diagnostic laboratories around the world, providing results that can be easily standardized between laboratories, within a few hours at a relative low-cost. There is no local production of commercial ELISA tests nor in Argentina neither in South America, therefore they must be purchased abroad, turning unfeasible the routinely use of ELISA tests. Here we have developed and validated accordingly to OIE specifications a novel ELISA test based on soluble antigens from N. caninum tachyzoites to accurately determine the presence of specific antibodies anti- N. caninum antigens in bovine sera samples, enabling the rapid, objective and low-cost diagnosis of bovine neosporosis in Argentina; replacing IFAT and avoiding delays and high importation taxes.es_AR
dc.formatapplication/pdfeng
dc.language.isoenges_AR
dc.publisherAnnex Publisherseng
dc.rightsinfo:eu-repo/semantics/openAccesseng
dc.sourceJournal of veterinary science and animal husbandry 7 (2) : 204. (August 29, 2019)es_AR
dc.subjectTécnicas Inmunológicases_AR
dc.subjectImmunological Techniqueseng
dc.subjectELISAes_AR
dc.subjectBovinaeng
dc.subjectBovinaees_AR
dc.subjectNeospora Caninumes_AR
dc.subjectAnticuerposes_AR
dc.subjectAntibodieseng
dc.subject.otherIgGeng
dc.subject.otherBovine Sera Sampleseng
dc.titleDevelopment and validation of a novel ELISA for the detection of Neospora caninum antibodies in bovine seraes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Balcarcees_AR
dc.description.filFil: Mansilla Florencia Celeste. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.es_AR
dc.description.filFil: Gual Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Pereyra Walter Rodrigo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Cardoso Nancy. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Turco Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.es_AR
dc.description.filFil: Capozzo Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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