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Abstract
The lack of naturally occurring resistance to citrus psorosis virus (CPsV) necessitates a transgenic approach for the development of CPsV-resistant citrus. To evaluate the feasibility of conferring resistance to a non-transgenic scion, we have assembled citrus plants by grafting combining a non-transgenic Sweet Orange as scion, CPsV-resistant transgenic Sweet Orange lines expressing intron-hairpin (ihp) RNA derived from the viral coat protein (ihpCP) as [ver mas...]
dc.contributor.authorde Francesco, Agustina
dc.contributor.authorSimeone, Melina
dc.contributor.authorGomez, Claudio Andres
dc.contributor.authorCosta, Norma Beatriz
dc.contributor.authorGarcia, Maria Laura
dc.date.accessioned2020-02-17T12:59:35Z
dc.date.available2020-02-17T12:59:35Z
dc.date.issued2020-01
dc.identifier.issn0962-8819
dc.identifier.issn1573-9368
dc.identifier.otherhttps://doi.org/10.1007/s11248-020-00191-1
dc.identifier.urihttp://hdl.handle.net/20.500.12123/6776
dc.identifier.urihttp://link.springer.com/10.1007/s11248-020-00191-1
dc.description.abstractThe lack of naturally occurring resistance to citrus psorosis virus (CPsV) necessitates a transgenic approach for the development of CPsV-resistant citrus. To evaluate the feasibility of conferring resistance to a non-transgenic scion, we have assembled citrus plants by grafting combining a non-transgenic Sweet Orange as scion, CPsV-resistant transgenic Sweet Orange lines expressing intron-hairpin (ihp) RNA derived from the viral coat protein (ihpCP) as interstock, and a non-transgenic citrus as rootstock. We demonstrated that ihpCP-transcripts translocate through the graft from interstock to scion, triggering the silencing of coat protein mRNA target. Two independent CPsV challenge assays showed that expression of ihpCP in the interstock provides resistance against CPsV in the interstock, and different levels of protection in the non-tg scion, depending of the virus delivery site. These results indicated that grafting is a promising biotechnological alternative to protect woody plants against virus infections in vegetative propagated plants.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherSpringeres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceTransgenic Research : 1-14 (January 2020)es_AR
dc.subjectCitruses_AR
dc.subjectNaranja Dulcees_AR
dc.subjectSweet Orangeseng
dc.subjectPlantas Transgénicases_AR
dc.subjectTransgenic Plantseng
dc.subjectResistencia a la Enfermedades_AR
dc.subjectDisease Resistanceeng
dc.subjectVirus de las Plantases_AR
dc.subjectPlant Viruseseng
dc.subject.otherVirus de la Psoriasis de los Cítricoses_AR
dc.subject.otherCitrus Psorosis Viruseng
dc.titleTransgenic Sweet Orange expressing hairpin CP-mRNA in the interstock confers tolerance to citrus psorosis virus in the non-transgenic sciones_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Concordiaes_AR
dc.description.filFil: de Francesco, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. University of California at Riverside. Department of Microbiology and Plant Pathology; Estados Unidoses_AR
dc.description.filFil: Simeone, M. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentinaes_AR
dc.description.filFil: Gomez, Claudio Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentinaes_AR
dc.description.filFil: Costa, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentinaes_AR
dc.description.filFil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentinaes_AR
dc.subtypecientifico


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