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Abstract
Low-cost high-throughput methods applicable to any virus strain are required for screening antiviral compounds against multiple field strains. Colorimetric cell-viability assays are used for this purpose as long as the viruses are cytopathic (CP) in cell culture. However, bovine viral diarrhoea virus (BVDV) strains circulating in the field are mostly non-cytopathic (NCP). An In Cell-ELISA aimed to measure viral infectivity by detecting a conserved protein [ver mas...]
dc.contributor.authorQuintana, Maria Eugenia
dc.contributor.authorBarone, Lucas
dc.contributor.authorForlenza, María Belén
dc.contributor.authorTrotta, Myrian Vanesa
dc.contributor.authorTurco, Cecilia
dc.contributor.authorMansilla, Florencia Celeste
dc.contributor.authorCardoso, Nancy Patricia
dc.contributor.authorCapozzo, Alejandra Victoria
dc.date.accessioned2019-12-13T10:53:45Z
dc.date.available2019-12-13T10:53:45Z
dc.date.issued2018-07-19
dc.identifier.issn0166-0934
dc.identifier.otherhttps://doi.org/10.1016/j.jviromet.2018.07.010
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0166093418301617
dc.identifier.urihttp://hdl.handle.net/20.500.12123/6503
dc.description.abstractLow-cost high-throughput methods applicable to any virus strain are required for screening antiviral compounds against multiple field strains. Colorimetric cell-viability assays are used for this purpose as long as the viruses are cytopathic (CP) in cell culture. However, bovine viral diarrhoea virus (BVDV) strains circulating in the field are mostly non-cytopathic (NCP). An In Cell-ELISA aimed to measure viral infectivity by detecting a conserved protein produced during viral replication (non-structural protein 3, “NS3”) was developed. The ELISA is performed without harvesting the cells, directly on the 96-wells culture plate. NS3 In Cell-ELISA was tested for its ability to assess BVDV-specific antiviral activity of recombinant bovine type I and III IFNs. Results correlated to those measured by qRT-PCR and virus titration. NS3 In Cell-ELISA was also efficient in estimating the IC50 of two compounds with different antiviral activity. Estimation of the 50% inhibition dose of each IFN using six BVDV strains of different biotype and genotype showed that CP strains were more susceptible to both IFNs than NCP, while type 2 NCP viruses were more sensitive to IFN-I. The In Cell-ELISA format using a detector antibody against a conserved non-structural protein can be potentially applied to accurately measure infectivity of any viral strain.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceJournal of Virological Methods 260 (1): 75–81. (October 2018)es_AR
dc.subjectBovine Viral Diarrhoeaeng
dc.subjectDiarrea Viral Bovinaes_AR
dc.subjectPathogenicityeng
dc.subjectPatogenicidades_AR
dc.subject.otherCytopathiceng
dc.subject.otherCell-ELISAeng
dc.titleA direct high-throughput in Cell-ELISA for measuring infectivity of cytopathic and non-cytopathic bovine viral diarrhoea virus strains applied to the assessment of antiviral activityes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Virologíaes_AR
dc.description.filFil: Quintana, María Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina.es_AR
dc.description.filFil: Barone, Lucas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.es_AR
dc.description.filFil: Forlenza, María Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.es_AR
dc.description.filFil: Trotta, Myrian Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.es_AR
dc.description.filFil: Turco, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.es_AR
dc.description.filFil: Mansilla, Florencia Celeste. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.es_AR
dc.description.filFil: Cardoso, Nancy Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina.es_AR
dc.description.filFil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina.es_AR
dc.subtypecientifico


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