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Abstract
Bovine herpesvirus-1 (BoHV-1) is the causative agent of bovine infectious rhinotracheitis, an important disease worldwide. Although conventional BoHV-1 vaccines, including those based on the use of modified live virus and also inactivated vaccines, are currently used in many countries, they have several disadvantages. DNA vaccines have emerged as an attractive approach since they have the potential to induce both humoral and cellular immune response; [ver mas...]
dc.contributor.authorQuattrocchi, Valeria
dc.contributor.authorSoria, Ivana
dc.contributor.authorLangellotti, Cecilia Ana
dc.contributor.authorGnazzo, Victoria
dc.contributor.authorGammella, Mariela
dc.contributor.authorMoore, Prando Dadin
dc.contributor.authorZamorano, Patricia Ines
dc.date.accessioned2017-07-03T15:44:21Z
dc.date.available2017-07-03T15:44:21Z
dc.date.issued2017-01-25
dc.identifier.otherhttps://doi.org/10.3389/fimmu.2017.00037
dc.identifier.urihttp://hdl.handle.net/20.500.12123/549
dc.identifier.urihttp://journal.frontiersin.org/article/10.3389/fimmu.2017.00037/full
dc.description.abstractBovine herpesvirus-1 (BoHV-1) is the causative agent of bovine infectious rhinotracheitis, an important disease worldwide. Although conventional BoHV-1 vaccines, including those based on the use of modified live virus and also inactivated vaccines, are currently used in many countries, they have several disadvantages. DNA vaccines have emerged as an attractive approach since they have the potential to induce both humoral and cellular immune response; nevertheless, it is largely known that potency of naked DNA vaccines is limited. We demonstrated previously, in the murine model, that the use of adjuvants in combination with a DNA vaccine against BoHV-1 is immunologically beneficial. In this study, we evaluate the immune response and protection against challenge elicited in bovines, by a DNA vaccine carrying the sequence of secreted version of glycoprotein D (gD) of BoHV-1 formulated with chemical adjuvants. Bovines were vaccinated with formulations containing the sequence of gD alone or in combination with adjuvants ESSAI 903110 or Montanide™ 1113101PR. After prime vaccination and two boosters, animals were challenged with infectious BoHV-1. Formulations containing adjuvants Montanide™ 1113101PR and ESSAI 903110 were both, capable of increasing humoral immune response against the virus and diminishing clinical symptoms. Nevertheless, only formulations containing adjuvant Montanide™ 1113101PR was capable of improving cellular immune response and diminishing viral excretion. To our knowledge, it is the first time that a BoHV-1 DNA vaccine is combined with adjuvants and tested in cattle. These results could be useful to design a vaccine for the control of bovine rhinotracheitis.eng
dc.formatapplication/pdf
dc.language.isoeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceFrontiers in immunology, January 2017
dc.subjectVirus herpes simplex
dc.subjectHerpes simplex viruseng
dc.subjectADN
dc.subjectVacuna
dc.subjectVaccineseng
dc.subjectGanado Bovino
dc.subjectCattleeng
dc.subjectAdjuvantseng
dc.subjectCoadyuvantes
dc.titleA DNA vaccine formulated with chemical adjuvant provides partial protection against bovine herpes virus infection in cattle
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Virología
dc.gic154039
dc.description.filFil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
dc.description.filFil: Soria, Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.filFil: Langellotti, Cecilia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.filFil: Gnazzo, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.filFil: Gammella, Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
dc.description.filFil: Moore, Prando Dadin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
dc.description.filFil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad del Salvador; Argentina
dc.subtypecientifico


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