Show simple item record

resumen

Abstract
The genus Ilex to which mate (Ilex paraguariensis) belongs, consists of more than 500 species. A wide range of metabolites including saponins and phenylpropanoids has been reported from Ilex species. However, despite the previous works on the Ilex metabolites, the metabolic similarities between species which can be used for chemotaxonomy of the species are not clear yet. In this study, nuclear magnetic resonance (NMR) spectroscopy-based metabolomics was [ver mas...]
dc.contributor.authorKim, Hye Kyong
dc.contributor.authorSaifullah
dc.contributor.authorKhan, Saifullah A.Jamil
dc.contributor.authorWilson, Erica Georgina
dc.contributor.authorPrat Kricun, Sergio
dc.contributor.authorMeissner, Axel
dc.contributor.authorGöraler, Sibel
dc.contributor.authorDeelder, André Martien
dc.contributor.authorChoi, Young-hae
dc.contributor.authorVerpoorte, Robert
dc.date.accessioned2019-07-15T13:43:44Z
dc.date.available2019-07-15T13:43:44Z
dc.date.issued2010-05
dc.identifier.issn0031-9422
dc.identifier.issn1873-3700
dc.identifier.otherhttps://doi.org/10.1016/j.phytochem.2010.02.001
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0031942210000427
dc.identifier.urihttp://hdl.handle.net/20.500.12123/5499
dc.description.abstractThe genus Ilex to which mate (Ilex paraguariensis) belongs, consists of more than 500 species. A wide range of metabolites including saponins and phenylpropanoids has been reported from Ilex species. However, despite the previous works on the Ilex metabolites, the metabolic similarities between species which can be used for chemotaxonomy of the species are not clear yet. In this study, nuclear magnetic resonance (NMR) spectroscopy-based metabolomics was applied to the classification of 11 South American Ilex species, namely, Ilex argentina, Ilex brasiliensis, Ilex brevicuspis, Ilex dumosa var. dumosa, I. dumosa var. guaranina, Ilex integerrima, Ilex microdonta, I. paraguariensis var. paraguariensis, Ilex pseudobuxus, Ilex taubertiana, and Ilex theezans. 1H NMR combined with principal component analysis (PCA), partial least square-discriminant analysis (PLS-DA) and hierarchical cluster analysis (HCA) showed a clear separation between species and resulted in four groups based on metabolomic similarities. The signal congestion of 1H NMR spectra was overcome by the implementation of two-dimensional (2D)-J-resolved and heteronuclear single quantum coherence (HSQC). From the results obtained by 1D- and 2D-NMR-based metabolomics it was concluded that species included in group A (I. paraguariensis) were metabolically characterized by a higher amount of xanthines, and phenolics including phenylpropanoids and flavonoids; group B (I. dumosa var. dumosa and I. dumosa var. guaranina) with oleanane type saponins; group C (I. brasiliensis, I. integerrima, I. pseudobuxus and I. theezans) with arbutin and dicaffeoylquinic acids, and group D (I. argentina, I. brevicuspis, I. microdonta and I. taubertiana) with the highest level of ursane-type saponins. Clear metabolomic discrimination of Ilex species and varieties in this study makes the chemotaxonomic classification of Ilex species possible.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourcePhytochemistry 71 (7) : 773-784 (May 2010)es_AR
dc.subjectIlexes_AR
dc.subjectIlex paraguariensises_AR
dc.subjectVariedadeses_AR
dc.subjectVarietieseng
dc.subjectMetabolismoes_AR
dc.subjectMetabolismeng
dc.subjectEspectroscopia RMNes_AR
dc.subjectNMR Spectroscopyeng
dc.subjectQuimiotaxonomíaes_AR
dc.subjectChemotaxonomyeng
dc.subjectAmérica del Sur
dc.subjectSouth Americaeng
dc.subject.otherEspectroscopia Resonancia Magnética Nucleares_AR
dc.titleMetabolic classification of South American Ilex species by NMR-based metabolomicses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Cerro Azules_AR
dc.description.filFil: Kim, Hye Kyong. Leiden University. Institute of Biology. Section Metabolomics. Division of Pharmacognosy; Holandaes_AR
dc.description.filFil: Saifullah. Leiden University. Institute of Biology. Section Metabolomics. Division of Pharmacognosy; Holanda. University of Karachi. International Center for Chemical and Biological Sciences. Research Institute of Chemistry. Biotechnology Wing H.E.J.; Paquistánes_AR
dc.description.filFil: Khan, Saifullah A.Jamil. University of Karachi. International Center for Chemical and Biological Sciences. Research Institute of Chemistry. Biotechnology Wing H.E.J.; Paquistánes_AR
dc.description.filFil: Wilson, Erica Georgina. Leiden University. Institute of Biology. Section Metabolomics. Division of Pharmacognosy; Holandaes_AR
dc.description.filFil: Prat Kricun, Sergio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cerro Azul; Argentinaes_AR
dc.description.filFil: Meissner, Axel. Leiden University Medical Center. Department of Parasitology; Holandaes_AR
dc.description.filFil: Göraler, Sibel. Leiden University Medical Center. Department of Parasitology; Holandaes_AR
dc.description.filFil: Deelder, André Martien. Leiden University Medical Center. Department of Parasitology; Holandaes_AR
dc.description.filFil: Choi, Young-hae. Leiden University. Institute of Biology. Section Metabolomics. Division of Pharmacognosy; Holandaes_AR
dc.description.filFil: Verpoorte, Robert. Leiden University. Institute of Biology. Section Metabolomics. Division of Pharmacognosy; Holandaes_AR
dc.subtypecientifico


Files in this item

Thumbnail

This item appears in the following Collection(s)

common

Show simple item record