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Resumen
Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of empty capsids as a subunit vaccine has been reported to be a promising candidate because it avoids the use of virus in the vaccine production and conserves the conformational epitopes of the virus. In this report, we explored [ver mas...]
dc.contributor.authorMignaqui, Ana Clara
dc.contributor.authorRuiz, Vanesa
dc.contributor.authorPerret, Sylvie
dc.contributor.authorSt-Laurent, Gilles
dc.contributor.authorChahal, Parminder Singh
dc.contributor.authorTransfiguracion, Julia
dc.contributor.authorSammarruco, Romina Ayelén
dc.contributor.authorGnazzo, Victoria
dc.contributor.authorDurocher, Yves
dc.contributor.authorWigdorovitz, Andres
dc.date.accessioned2019-04-03T17:36:27Z
dc.date.available2019-04-03T17:36:27Z
dc.date.issued2013-08-20
dc.identifier.issn1932-6203
dc.identifier.otherhttps://doi.org/10.1371/journal.pone.0072800
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4809
dc.identifier.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0072800
dc.description.abstractFoot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of empty capsids as a subunit vaccine has been reported to be a promising candidate because it avoids the use of virus in the vaccine production and conserves the conformational epitopes of the virus. In this report, we explored transient gene expression (TGE) in serum-free suspension-growing mammalian cells for the production of FMDV recombinant empty capsids as a subunit vaccine. The recombinant proteins produced, assembled into empty capsids and induced protective immune response against viral challenge in mice. Furthermore, they were recognized by anti-FMDV bovine sera. By using this technology, we were able to achieve expression levels that are compatible with the development of a vaccine. Thus, TGE of mammalian cells is an easy to perform, scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.publisherPLOS
dc.relationinfo:eu-repograntAgreement/INTA/AESA/201721/AR./Fiebre aftosaes_AR
dc.rightsinfo:eu-repo/semantics/openAccesseng
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.sourcePLoS ONE 8 (8) : e72800. (August 20, 2013)es_AR
dc.subjectExpresión Génicaes_AR
dc.subjectGene Expressioneng
dc.subjectMamíferoses_AR
dc.subjectMammalseng
dc.subjectVirus Fiebre Aftosaes_AR
dc.subjectAphthoviruseng
dc.subjectRecombinaciónes_AR
dc.subjectRecombinationeng
dc.subjectTransfectioneng
dc.subjectTransfecciónes_AR
dc.subjectVaccineseng
dc.subjectVacunaes_AR
dc.subjectProteaseseng
dc.subjectProteasases_AR
dc.subject.otherFoot and Mouth Disease Viruseng
dc.subject.otherCapsidseng
dc.subject.otherCápsidases_AR
dc.titleTransient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsidseng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.rights.licenseCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.description.origenInstituto de Virologíaes_AR
dc.description.filFil: Mignaqui, Ana Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentinaes_AR
dc.description.filFil: Perret, Sylvie. National Research Council of Canada. Biotechnology Research Institute; Canadáes_AR
dc.description.filFil: St-Laurent, Gilles. National Research Council of Canada. Biotechnology Research Institute; Canadáes_AR
dc.description.filFil: Chahal, Parminder Singh. National Research Council of Canada. Biotechnology Research Institute; Canadáes_AR
dc.description.filFil: Transfiguracion, Julia. National Research Council of Canada. Biotechnology Research Institute; Canadáes_AR
dc.description.filFil: Sammarruco, Romina Ayelén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentinaes_AR
dc.description.filFil: Gnazzo, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Durocher, Yves. National Research Council of Canada. Biotechnology Research Institute; Canadáes_AR
dc.description.filFil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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