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Abstract
The aim of this study was to evaluate the response of orange fruit (Citrus sinensis var. Valencia Late) to freezing stress in planta, both immediately after the natural event and after a week, in order to understand the biochemical and molecular basis of the changes that later derive in internal and external damage symptoms. Using two‐dimensional differential gel electrophoresis to analyze exposed and non‐exposed fruit, 27 differential protein spots were [ver mas...]
dc.contributor.authorPerotti, Valeria Elisa
dc.contributor.authorMoreno, Alejandra Soledad
dc.contributor.authorTripodi, Karina Eva Josefina
dc.contributor.authorMeier, Guillermo Enrique
dc.contributor.authorBello, Fernando
dc.contributor.authorCocco, Mariángeles
dc.contributor.authorVazquez, Daniel Eduardo
dc.contributor.authorAnderson, Catalina Margarita
dc.contributor.authorPodesta, Florencio Esteban
dc.date.accessioned2019-03-19T13:29:18Z
dc.date.available2019-03-19T13:29:18Z
dc.date.issued2015-03
dc.identifier.issn0031-9317
dc.identifier.issn1399-3054
dc.identifier.otherhttps://doi.org/10.1111/ppl.12259
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/10.1111/ppl.12259
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4653
dc.description.abstractThe aim of this study was to evaluate the response of orange fruit (Citrus sinensis var. Valencia Late) to freezing stress in planta, both immediately after the natural event and after a week, in order to understand the biochemical and molecular basis of the changes that later derive in internal and external damage symptoms. Using two‐dimensional differential gel electrophoresis to analyze exposed and non‐exposed fruit, 27 differential protein spots were detected in juice sacs and flavedo, among all comparisons made. Also, primary and secondary metabolites relative contents were analyzed in both tissues by gas chromatography‐mass spectrometry and liquid chromatography‐mass spectrometry, respectively. Proteins and compounds involved in regulatory functions, iron metabolism, oxidative damage and carbohydrate metabolism were the most affected. Interestingly, three glycolytic enzymes were induced by cold, and there was an increase in fermentation products (volatiles); all of that suggests that more energy generation might be required from glycolysis to counter the cold stress. Moreover, a notable increase in sugar levels was observed after frost, but it was not at the expense of organic acids utilization. Consequently, these results suggest a probable redistribution of photoassimilates in the frost‐exposed plants, tending to restore the homeostasis altered by that severe type of stress. Isosinensetin was the most cold‐sensitive secondary metabolite because it could not be detected at all after the frost, constituting a possible tool to early diagnose freezing damage.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherWileyes_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourcePhysiologia Plantarum 153 (3) : 337-354 (March 2015)es_AR
dc.subjectNaranja Dulcees_AR
dc.subjectSweet Orangeseng
dc.subjectVariedadeses_AR
dc.subjectVarietieseng
dc.subjectCitruses_AR
dc.subjectHeladaes_AR
dc.subjectFrosteng
dc.subjectEstrés de Fríoes_AR
dc.subjectCold Stresseng
dc.subjectMetabolismoes_AR
dc.subjectMetabolismeng
dc.subject.otherVariedad Valenciaes_AR
dc.subject.otherProteomicses_AR
dc.titleProteomic and metabolomic profiling of Valencia orange fruit after natural frost exposurees_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Concordiaes_AR
dc.description.filFil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentinaes_AR
dc.description.filFil: Moreno, Alejandra Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentinaes_AR
dc.description.filFil: Tripodi, Karina Eva Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentinaes_AR
dc.description.filFil: Meier, Guillermo Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentinaes_AR
dc.description.filFil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentinaes_AR
dc.description.filFil: Cocco, Mariángeles. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentinaes_AR
dc.description.filFil: Vazquez, Daniel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentinaes_AR
dc.description.filFil: Anderson, Catalina Margarita. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentinaes_AR
dc.description.filFil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentinaes_AR
dc.subtypecientifico


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