Show simple item record

resumen

Abstract
Exosomes are 60–150 nm small extracellular vesicles (EVs) released by most cells. Tumor-cell-derived exosomes, used as a vaccine, elicit a specific cytotoxic response against tumor cells, usually with a greater immunogenicity than tumor-cell lysates. However, the number of exosomes isolated from culture cells is limited. In recent studies, it was observed that cells respond to different stressor stimuli such as cytotoxic drugs, hypoxia, acidosis, or [ver mas...]
dc.contributor.authorCocozza, Federico
dc.contributor.authorMenay, Florencia
dc.contributor.authorTsacalian, Rodrigo Farid
dc.contributor.authorElisei, Analia
dc.contributor.authorSampedro, Pura
dc.contributor.authorSoria, Ivana
dc.contributor.authorWaldner, Claudia Inés
dc.contributor.authorGravisaco, María José
dc.contributor.authorMongini, Claudia
dc.date.accessioned2019-03-13T13:08:41Z
dc.date.available2019-03-13T13:08:41Z
dc.date.issued2019-03
dc.identifier.issn0264-410X
dc.identifier.otherhttps://doi.org/10.1016/j.vaccine.2019.02.004
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4586
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0264410X19301872?via%3Dihub
dc.description.abstractExosomes are 60–150 nm small extracellular vesicles (EVs) released by most cells. Tumor-cell-derived exosomes, used as a vaccine, elicit a specific cytotoxic response against tumor cells, usually with a greater immunogenicity than tumor-cell lysates. However, the number of exosomes isolated from culture cells is limited. In recent studies, it was observed that cells respond to different stressor stimuli such as cytotoxic drugs, hypoxia, acidosis, or radiation by increasing the release of EVs. In this study, using the murine LBC T-cell lymphoma, we found that cyclophosphamide significantly increased EVs yield. These EVs express exosome marker proteins such as TSG-101, CD9, CD81, and CD63. Furthermore, similar humoral and cellular immune responses were induced in vivo by EVs isolated from LBC-tumor cells whether they were grown under normal culture conditions (EVs C) or in the presence of cyclophosphamide (EVs CTX). Mice vaccinated either with EVs C or EVs CTX were similarly protected against an intraperitoneal challenge with LBC tumor cells. CD4+ and CD8+ IFN-c secreting cells were induced in immunized mice and a specific cytotoxic cellular immune response was elicited in vitro. These results demonstrate that a Th1 response was induced by immunization with the EVs. Our findings suggest that treatment of tumor cells with cyclophosphamide is a useful method to enhance the secretion of EVs in sensitive cell lines without altering their antitumor properties and thus may be used to produce antigens for future design of cancer vaccines.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.publisherElseviereng
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceVaccine 37 (12) : 1565-1576. (14 March 2019)eng
dc.subjectCiclofosfamidaes_AR
dc.subjectCyclophosphamideeng
dc.subjectNeoplasmases_AR
dc.subjectNeoplasmseng
dc.subjectRespuesta Inmunológicaes_AR
dc.subjectImmune Responseeng
dc.subjectLinfomaes_AR
dc.subjectLymphomaeng
dc.subjectVaccineseng
dc.subjectVacunaes_AR
dc.subjectT-lymphocyteseng
dc.subjectLinfocitos-tes_AR
dc.subject.otherExtracellular Vesicleseng
dc.subject.otherVesículas Extracelulareses_AR
dc.subject.otherExosomeseng
dc.subject.otherExosomases_AR
dc.titleCyclophosphamide enhances the release of tumor exosomes that elicit a specific immune response in vivo in a murine T-cell lymphomaeng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Virologíaes_AR
dc.description.filFil: Cocozza, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentinaes_AR
dc.description.filFil: Menay, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentinaes_AR
dc.description.filFil: Tsacalian, Rodrigo Farid. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentinaes_AR
dc.description.filFil: Elisei, Analia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentinaes_AR
dc.description.filFil: Sampedro, Pura. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentinaes_AR
dc.description.filFil: Soria, Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Waldner, Claudia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentinaes_AR
dc.description.filFil: Gravisaco, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Mongini, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentinaes_AR
dc.subtypecientifico


Files in this item

Thumbnail

This item appears in the following Collection(s)

common

Show simple item record