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Abstract
Heat treatment induces defense mechanisms and triggers physiological responses that allow citrus fruit to withstand stressful conditions during storage. The aim of this paper was to identify changes in the proteome and the biochemistry of Valencia oranges (Citrus sinensis cv Valencia late) subjected to heat treatment before 0, 30 and 60 d of storage at 5 °C. Heat treatment for 48 h at 37 °C and 90% relative humidity was followed by 24 h at 20 °C. [ver mas...]
dc.contributor.authorPerotti, Valeria Elisa
dc.contributor.authordel Vecchio, Hernán A.
dc.contributor.authorSansevich, Alina
dc.contributor.authorMeier, Guillermo Enrique
dc.contributor.authorBello, Fernando
dc.contributor.authorCocco, Mariángeles
dc.contributor.authorGarran, Sergio Mario
dc.contributor.authorAnderson, Catalina Margarita
dc.contributor.authorVazquez, Daniel Eduardo
dc.contributor.authorPodesta, Florencio Esteban
dc.date.accessioned2019-03-07T14:45:35Z
dc.date.available2019-03-07T14:45:35Z
dc.date.issued2011-11
dc.identifier.issn0925-5214
dc.identifier.otherhttps://doi.org/10.1016/j.postharvbio.2011.05.015
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0925521411001323
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4545
dc.description.abstractHeat treatment induces defense mechanisms and triggers physiological responses that allow citrus fruit to withstand stressful conditions during storage. The aim of this paper was to identify changes in the proteome and the biochemistry of Valencia oranges (Citrus sinensis cv Valencia late) subjected to heat treatment before 0, 30 and 60 d of storage at 5 °C. Heat treatment for 48 h at 37 °C and 90% relative humidity was followed by 24 h at 20 °C. Differentially expressed proteins were detected by two-dimensional differential gel electrophoresis and identified by matrix-assisted laser desorption/ionization MS. Twenty eight different proteins in 40 spots among the 61 analyzed were identified. Functional classification showed that the main affected categories were “Cell rescue, defense, and virulence” and “Metabolism”; while 20% were found to be storage proteins with the rest related to minor categories. Activity of antioxidant enzymes was also evaluated. The most conspicuous change was an increase in superoxide dismutase in both the flavedo and juice sacs. Peroxidases increased in flavedo but decreased in sacs while alcohol dehydrogenase increased in treated flavedo, but not in sacs. Other fermentative enzymes were not affected. Metabolite content showed a higher concentration of sugars in heat treated fruit, while the main acids were slightly or not affected. These results are consistent with the development of a lower degree of fruit susceptibility to fungal pathogens, thus explaining the maintenance of postharvest quality.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourcePostharvest Biology and Technology 62 (2) : 97-114 (November 2011)es_AR
dc.subjectNaranja Dulcees_AR
dc.subjectSweet Orangeseng
dc.subjectVariedadeses_AR
dc.subjectVarietieseng
dc.subjectTratamiento Térmicoes_AR
dc.subjectHeat Treatmenteng
dc.subjectCitruses_AR
dc.subjectTecnología Postcosechaes_AR
dc.subjectPostharvest Technologyeng
dc.subject.otherProteomicses_AR
dc.titleProteomic, metabalomic, and biochemical analysis of heat treated Valencia oranges during storagees_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Concordiaes_AR
dc.description.filFil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentinaes_AR
dc.description.filFil: del Vecchio, Hernán A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentinaes_AR
dc.description.filFil: Sansevich, Alina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentinaes_AR
dc.description.filFil: Meier, Guillermo Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentinaes_AR
dc.description.filFil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentinaes_AR
dc.description.filFil: Cocco, Mariángeles. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentinaes_AR
dc.description.filFil: Garran, Sergio Mario. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentinaes_AR
dc.description.filFil: Anderson, Catalina Margarita. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentinaes_AR
dc.description.filFil: Vazquez, Daniel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentinaes_AR
dc.description.filFil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentinaes_AR
dc.subtypecientifico


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