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Abstract
Previous attempts to produce a vaccine against BLV faced problems of efficacy (i.e. only a fraction of animals were protected), persistence (i.e. rapid decrease of immune protection), cost (e.g. production of purified proteins) or safety (e.g. genetically modified hybrid viruses). We have designed a novel strategy based on the use of a live-attenuated BLV provirus. The rationale behind this strategy relies on the deletion of genes required to induce [ver mas...]
dc.contributor.authorGutierrez, Gerónimo
dc.contributor.authorRodríguez, Sabrina M.
dc.contributor.authorVilor, Agustín
dc.contributor.authorGillet, Nicolas A.
dc.contributor.authorDeBrogniez, Alix
dc.contributor.authorTrono, Karina Gabriela
dc.contributor.authorWillems, Luc
dc.date.accessioned2019-02-20T18:14:10Z
dc.date.available2019-02-20T18:14:10Z
dc.date.issued2015
dc.identifier.issn1742-4690
dc.identifier.other10.1186/1742-4690-12-S1-I1
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4477
dc.identifier.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4578685/
dc.description17th International Conference on Human Retroviruses: HTLV and Related Viruses, Trois Ilets, Martinique,(FWI). 18-21 June 2015es_AR
dc.description.abstractPrevious attempts to produce a vaccine against BLV faced problems of efficacy (i.e. only a fraction of animals were protected), persistence (i.e. rapid decrease of immune protection), cost (e.g. production of purified proteins) or safety (e.g. genetically modified hybrid viruses). We have designed a novel strategy based on the use of a live-attenuated BLV provirus. The rationale behind this strategy relies on the deletion of genes required to induce pathogenesis maintaining integrity of those involved in infectivity. We have identified a BLV deleted provirus that is infectious in cattle but replicates at reduced levels in cows as shown by real-time quantitative PCR. The deletant elicits a strong anti-BLV immune response as indicated by wild-type antibody titers. Vaccinated animals but not uninfected controls resist challenge by a wild type BLV virus. The deletant does not spread to uninfected sentinels maintained during 5 years in the same herd supporting biosafety of the vaccine. Passive immunity, but not viral infection, is transmitted to the newborn calves via the maternal colostrum. Assays regarding production, storage and delivery of the vaccine, as well as safety of the milk produced by vaccinated cows are currently being carried out with the aim to start a large scale trial that will be held in Argentina in real dairy conditions during this year.es_AR
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherBioMed Centrales_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.sourceRetrovirology 12 (Suppl 1) : P3. (2015)es_AR
dc.subjectLeucemiaes_AR
dc.subjectLeukaemiaes_AR
dc.subjectVirus Leucemia Bovinaes_AR
dc.subjectBovine Leukaemia Viruses_AR
dc.subjectVacunaes_AR
dc.subjectVaccineses_AR
dc.titleAn efficient vaccine against bovine leukemia viruses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.rights.licenseCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.description.origenInstituto de Virologíaes_AR
dc.description.filFil: Gutierrez, Gerónimo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentinaes_AR
dc.description.filFil: Rodríguez, Sabrina M. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Belgicaes_AR
dc.description.filFil: Vilor, Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentinaes_AR
dc.description.filFil: Gillet, Nicolas A. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Belgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; Belgicaes_AR
dc.description.filFil: DeBrogniez, Alix. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Belgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; Belgicaes_AR
dc.description.filFil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentinaes_AR
dc.description.filFil: Willems, Luc. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Belgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; Belgicaes_AR
dc.subtypecientifico


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