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resumen

Resumen
Analysis of the immune response to infection of livestock by foot-and-mouth disease virus (FMDV) is most often reported as the serum antibody response to the virus. While measurement of neutralizing antibody has been sensitive and specific, measurements of the quality of the antibody response are less robust. Determining the immunoglobulin (Ig) isotype of the serum antibody response provides a deeper understanding of the biology of the response and more [ver mas...]
dc.contributor.authorKenney, Mary
dc.contributor.authorWaters, Ryan A.
dc.contributor.authorRieder, Elizabeth
dc.contributor.authorPega, Juan Franco
dc.contributor.authorPerez Filgueira, Daniel Mariano
dc.contributor.authorGolde, William T.
dc.date.accessioned2019-02-14T15:50:37Z
dc.date.available2019-02-14T15:50:37Z
dc.date.issued2017-11
dc.identifier.issn0022-1759
dc.identifier.otherhttps://doi.org/10.1016/j.jim.2017.07.001
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4444
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0022175917302144?via%3Dihub
dc.description.abstractAnalysis of the immune response to infection of livestock by foot-and-mouth disease virus (FMDV) is most often reported as the serum antibody response to the virus. While measurement of neutralizing antibody has been sensitive and specific, measurements of the quality of the antibody response are less robust. Determining the immunoglobulin (Ig) isotype of the serum antibody response provides a deeper understanding of the biology of the response and more sensitive methods for these assays will facilitate analyses of B cell mediated immunity. We tested the hypothesis that using the virus as the molecular probe could be achieved by adding tags to the surface of the FMDV capsid, and that would enhance sensitivity in assays for anti-FMDV antibody responses. The use of a FLAG-tagged virus in these assays failed to yield improvement whereas chemically biotinylating the virus capsid resulted in significant enhancement of the signal. Here we describe methods using biotinylated virus for measuring anti-viral antibody in serum and antibody secreting cells (ASCs) in blood that are sensitive and specific. Finally, we describe using the biotinylated virus in flow cytometry where such assays should greatly enhance the analysis of anti-virus antibody producing B cells, allowing the investigator to focus on only the FMDV specific B cells when analyzing the development of the B cell response to either infection or vaccination.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.publisherElseviereng
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceJournal of immunological methods 450 : 1-9. (November 2017)eng
dc.subjectVirus Fiebre Aftosaes_AR
dc.subjectAphthoviruseng
dc.subjectRespuesta Inmunológicaes_AR
dc.subjectImmune Responseeng
dc.subjectELISAes_AR
dc.subjectFlow Cytometryeng
dc.subjectCitometría de Flujo (Células)es_AR
dc.subject.otherFoot and Mouth Disease Viruseng
dc.subject.otherBiotinylationeng
dc.subject.otherBiotinilaciónes_AR
dc.titleEnhanced sensitivity in detection of antiviral antibody responses using biotinylation of foot-and-mouth disease virus (FMDV) capsidseng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Virologíaes_AR
dc.description.filFil: Kenney, Mary. United States Department of Agriculture. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidoses_AR
dc.description.filFil: Waters, Ryan A. United States Department of Agriculture. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidoses_AR
dc.description.filFil: Rieder, Elizabeth. United States Department of Agriculture. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidoses_AR
dc.description.filFil: Pega, Juan Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Perez Filgueira, Daniel Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Golde, William T. United States Department of Agriculture. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidoses_AR
dc.subtypecientifico


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