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Foot-and-mouth disease (FMD) remains one of the most feared viral diseases affecting cloven-hoofed animals, and results in severe economic losses. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of recombinant FMDV-like particles (VLPs) as subunit vaccines has gained importance because of their immunogenic properties and safety. We evaluated the production of FMD VLPs, via Agrobacterium-mediated transient expression, and
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dc.contributor.author | Ruiz, Vanesa | |
dc.contributor.author | Baztarrica, Josefina | |
dc.contributor.author | Rybicki, Edward P. | |
dc.contributor.author | Meyers, Ann E. | |
dc.contributor.author | Wigdorovitz, Andres | |
dc.date.accessioned | 2019-02-14T15:10:30Z | |
dc.date.available | 2019-02-14T15:10:30Z | |
dc.date.issued | 2018-12 | |
dc.identifier.issn | 2215-017X | |
dc.identifier.other | https://doi.org/10.1016/j.btre.2018.e00283 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/4443 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S2215017X1830050X?via%3Dihub | |
dc.description.abstract | Foot-and-mouth disease (FMD) remains one of the most feared viral diseases affecting cloven-hoofed animals, and results in severe economic losses. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of recombinant FMDV-like particles (VLPs) as subunit vaccines has gained importance because of their immunogenic properties and safety. We evaluated the production of FMD VLPs, via Agrobacterium-mediated transient expression, and the immunogenicity of these structures in mice. Leaves were infiltrated with pEAQ-HT and pRIC 3.0 vectors encoding the capsid precursor P1-2A and the protease 3C. The recombinant protein yield was 3–4 mg/kg of fresh leaf tissue. Both groups of mice immunized with purified VLPs and mice immunized with the crude leaf extract elicited a specific humoral response with similar antibody titers. Thus, minimally processed plant material containing transiently expressed FMD VLPs could be a scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV. | eng |
dc.format | application/pdf | eng |
dc.language.iso | eng | |
dc.publisher | Elsevier | eng |
dc.rights | info:eu-repo/semantics/restrictedAccess | eng |
dc.source | Biotechnology reports 20 : e00283. (December 2018) | eng |
dc.subject | Extractos de Hoja | es_AR |
dc.subject | Leaf Extracts | eng |
dc.subject | Virus Fiebre Aftosa | es_AR |
dc.subject | Aphthovirus | eng |
dc.subject | Inmunogenética | es_AR |
dc.subject | immunogenetics | eng |
dc.subject | Mice | eng |
dc.subject | Ratón | es_AR |
dc.subject.other | Nicotiana Benthamiana | es_AR |
dc.subject.other | Foot and Mouth Disease Virus | eng |
dc.title | Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice | eng |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | eng |
dc.type | info:eu-repo/semantics/publishedVersion | eng |
dc.description.origen | Instituto de Virología | es_AR |
dc.description.fil | Fil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina | es_AR |
dc.description.fil | Fil: Baztarrica, Josefina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina | es_AR |
dc.description.fil | Fil: Rybicki, Edward P. University of Cape Town. Department of Molecular and cell Biology. Biopharming Research Unit; Sudáfrica. University of Cape Town. Faculty of Health Sciences. Institute of Infectious Disease and Molecular Medicine; Sudáfrica | es_AR |
dc.description.fil | Fil: Meyers, Ann E. University of Cape Town. Department of Molecular and cell Biology. Biopharming Research Unit; Sudáfrica | es_AR |
dc.description.fil | Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.subtype | cientifico |
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