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Abstract
Foot-and-mouth disease (FMD) remains one of the most feared viral diseases affecting cloven-hoofed animals, and results in severe economic losses. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of recombinant FMDV-like particles (VLPs) as subunit vaccines has gained importance because of their immunogenic properties and safety. We evaluated the production of FMD VLPs, via Agrobacterium-mediated transient expression, and [ver mas...]
dc.contributor.authorRuiz, Vanesa
dc.contributor.authorBaztarrica, Josefina
dc.contributor.authorRybicki, Edward P.
dc.contributor.authorMeyers, Ann E.
dc.contributor.authorWigdorovitz, Andres
dc.date.accessioned2019-02-14T15:10:30Z
dc.date.available2019-02-14T15:10:30Z
dc.date.issued2018-12
dc.identifier.issn2215-017X
dc.identifier.otherhttps://doi.org/10.1016/j.btre.2018.e00283
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4443
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S2215017X1830050X?via%3Dihub
dc.description.abstractFoot-and-mouth disease (FMD) remains one of the most feared viral diseases affecting cloven-hoofed animals, and results in severe economic losses. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of recombinant FMDV-like particles (VLPs) as subunit vaccines has gained importance because of their immunogenic properties and safety. We evaluated the production of FMD VLPs, via Agrobacterium-mediated transient expression, and the immunogenicity of these structures in mice. Leaves were infiltrated with pEAQ-HT and pRIC 3.0 vectors encoding the capsid precursor P1-2A and the protease 3C. The recombinant protein yield was 3–4 mg/kg of fresh leaf tissue. Both groups of mice immunized with purified VLPs and mice immunized with the crude leaf extract elicited a specific humoral response with similar antibody titers. Thus, minimally processed plant material containing transiently expressed FMD VLPs could be a scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.publisherElseviereng
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceBiotechnology reports 20 : e00283. (December 2018)eng
dc.subjectExtractos de Hojaes_AR
dc.subjectLeaf Extractseng
dc.subjectVirus Fiebre Aftosaes_AR
dc.subjectAphthoviruseng
dc.subjectInmunogenéticaes_AR
dc.subjectimmunogeneticseng
dc.subjectMiceeng
dc.subjectRatónes_AR
dc.subject.otherNicotiana Benthamianaes_AR
dc.subject.otherFoot and Mouth Disease Viruseng
dc.titleMinimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in miceeng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Virologíaes_AR
dc.description.filFil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentinaes_AR
dc.description.filFil: Baztarrica, Josefina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentinaes_AR
dc.description.filFil: Rybicki, Edward P. University of Cape Town. Department of Molecular and cell Biology. Biopharming Research Unit; Sudáfrica. University of Cape Town. Faculty of Health Sciences. Institute of Infectious Disease and Molecular Medicine; Sudáfricaes_AR
dc.description.filFil: Meyers, Ann E. University of Cape Town. Department of Molecular and cell Biology. Biopharming Research Unit; Sudáfricaes_AR
dc.description.filFil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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