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Recombinant FMDV empty capsids have been produced in insect cells and larvae using the baculovirus expression system, although protein yield and efficiency of capsid assembly have been highly variable. In this work, two strategies were compared for the expression of FMDV A/Arg/01 empty capsids: infection with a dual-promoter baculovirus vector coding for the capsid precursor (P12A) and the protease 3C under the control of the polyhedrin and p10 promoters,
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dc.contributor.author | Ruiz, Vanesa | |
dc.contributor.author | Mignaqui, Ana Clara | |
dc.contributor.author | Nuñez, M.C. | |
dc.contributor.author | Reytor, Edel | |
dc.contributor.author | Escribano, José M. | |
dc.contributor.author | Wigdorovitz, Andres | |
dc.date.accessioned | 2019-01-22T14:20:29Z | |
dc.date.available | 2019-01-22T14:20:29Z | |
dc.date.issued | 2014-11 | |
dc.identifier.issn | 1073-6085 | |
dc.identifier.issn | 1559-0305 | |
dc.identifier.other | https://doi.org/10.1007/s12033-014-9775-8 | |
dc.identifier.uri | https://link.springer.com/article/10.1007/s12033-014-9775-8 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/4310 | |
dc.description.abstract | Recombinant FMDV empty capsids have been produced in insect cells and larvae using the baculovirus expression system, although protein yield and efficiency of capsid assembly have been highly variable. In this work, two strategies were compared for the expression of FMDV A/Arg/01 empty capsids: infection with a dual-promoter baculovirus vector coding for the capsid precursor (P12A) and the protease 3C under the control of the polyhedrin and p10 promoters, respectively (BacP12A-3C), or a single-promoter vector coding the P12A3C cassette (BacP12A3C). Expression levels and assembly into empty capsids were analyzed in insect cells and larvae. We observed that the use of the single-promoter vector allowed higher levels of expression both in insect cells and larvae. Recombinant capsid proteins produced by both vectors were recognized by monoclonal antibodies (mAbs) directed against conformational epitopes of FMDV A/Arg/01 and proved to self-assemble into empty capsids (75S) and pentamers (12S) when analyzed by sucrose gradient centrifugation. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | Springer | es_AR |
dc.rights | info:eu-repo/semantics/restrictedAccess | es_AR |
dc.source | Molecular Biotechnology 56 (11) : 963–970 (November 2014) | es_AR |
dc.subject | Enfermedades de los Animales | es_AR |
dc.subject | Animal Diseases | eng |
dc.subject | Fiebre Aftosa | es_AR |
dc.subject | Foot and Mouth Disease | eng |
dc.subject | Virus Fiebre Aftosa | es_AR |
dc.subject | Aphthovirus | eng |
dc.subject | Baculovirus | es_AR |
dc.subject | Células | es_AR |
dc.subject | Cells | eng |
dc.title | Comparison of strategies for the production of FMDV empty capsids using the Baculovirus Vector System | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.description.origen | Instituto de Virología | es_AR |
dc.description.fil | Fil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Mignaqui, Ana Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Nuñez, M.C. Universidad Politécnica de Madrid. Parque Científico y Tecnológico. Alternative Gene Expression S.L. (ALGENEX); España | es_AR |
dc.description.fil | Fil: Reytor, Edel. Universidad Politécnica de Madrid. Parque Científico y Tecnológico. Alternative Gene Expression S.L. (ALGENEX); España | es_AR |
dc.description.fil | Fil: Escribano, José M. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA). Departamento de Biotecnología; España | es_AR |
dc.description.fil | Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.subtype | cientifico |
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