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Abstract
Background: Bovine herpesvirus type 1 (BoHV-1) is the causative agent of respiratory and genital tract infections; causing a high economic loss in all continents. Use of marker vaccines in IBR eradication programs is widely accepted since it allows for protection of the animals against the disease while adding the possibility of differentiating vaccinated from infected animals. The aim of the present study was the development and evaluation of safety and [ver mas...]
dc.contributor.authorRomera, Sonia Alejandra
dc.contributor.authorPuntel, Mariana
dc.contributor.authorQuattrocchi, Valeria
dc.contributor.authorDel Medico Zajac, Maria Paula
dc.contributor.authorZamorano, Patricia Ines
dc.contributor.authorBlanco Viera, Francisco Javier
dc.contributor.authorCarrillo, Consuelo
dc.contributor.authorChowdhury, Shafiqul
dc.contributor.authorBorca, Manuel Victor
dc.contributor.authorSadir, Ana Maria
dc.date.accessioned2019-01-22T13:40:17Z
dc.date.available2019-01-22T13:40:17Z
dc.date.issued2014-01
dc.identifier.issn1746-6148
dc.identifier.otherhttps://doi.org/10.1186/1746-6148-10-8
dc.identifier.urihttps://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-10-8
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4309
dc.description.abstractBackground: Bovine herpesvirus type 1 (BoHV-1) is the causative agent of respiratory and genital tract infections; causing a high economic loss in all continents. Use of marker vaccines in IBR eradication programs is widely accepted since it allows for protection of the animals against the disease while adding the possibility of differentiating vaccinated from infected animals. The aim of the present study was the development and evaluation of safety and efficacy of a glycoprotein E-deleted (gE-) BoHV-1 marker vaccine strain (BoHV-1ΔgEβgal) generated by homologous recombination, replacing the viral gE gene with the β-galactosidase (βgal) gene. Results: In vitro growth kinetics of the BoHV-1ΔgEβgal virus was similar to BoHV-1 LA. The immune response triggered by the new recombinant strain in cattle was characterized both as live attenuated vaccine (LAV) and as an inactivated vaccine. BoHV-1ΔgEβgal was highly immunogenic in both formulations, inducing specific humoral and cellular immune responses. Antibody titers found in animals vaccinated with the inactivated vaccine based on BoHV-1ΔgEβgal was similar to the titers found for the control vaccine (BoHV-1 LA). In the same way, titers of inactivated vaccine groups were significantly higher than any of the LAV immunized groups, independently of the inoculation route (p < 0.001). Levels of IFN-γ were significantly higher (p < 0.001) in those animals that received the LAV compared to those that received the inactivated vaccine. BoHV-1ΔgEβgal exhibited an evident attenuation when administered as a LAV; no virus was detected in nasal secretions of vaccinated or sentinel animals during the post-vaccination period. BoHV-1ΔgEβgal, when used in either formulation, elicited an efficient immune response that protected animals against challenge with virulent wild-type BoHV-1. Also, the deletion of the gE gene served as an immunological marker to differentiate vaccinated animals from infected animals. All animals vaccinated with the BoHV-1ΔgE βgal strain were protected against disease after challenge and shed significantly less virus than control calves, regardless of the route and formulation they were inoculated. Conclusions: Based on its attenuation, immunogenicity and protective effect after challenge, BoHV-1ΔgEβgal virus is an efficient and safe vaccine candidate when used either as inactivated or as live attenuated forms.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherBMCes_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceBMC Veterinary Research 10 : 8 (2014)es_AR
dc.subjectEnfermedades de los Animaleses_AR
dc.subjectAnimal Diseaseseng
dc.subjectGanado Bovinoes_AR
dc.subjectCattleeng
dc.subjectHerpes Virus Bovinoes_AR
dc.subjectBovine Herpesviruseng
dc.subjectGlicoproteínases_AR
dc.subjectGlycoproteinseng
dc.subjectVacunaes_AR
dc.subjectVaccineseng
dc.subjectVacuna Vivaes_AR
dc.subjectLive Vaccineseng
dc.subjectVacuna Inactivadaes_AR
dc.subjectInactivated Vaccineseng
dc.titleProtection induced by a glycoprotein E-deleted bovine herpesvirus type 1 marker strain used either as an inactivated or live attenuated vaccine in cattlees_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Virologíaes_AR
dc.description.filFil: Romera, Sonia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad del Salvador. Cátedra de Inmunología; Argentinaes_AR
dc.description.filFil: Puntel, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentinaes_AR
dc.description.filFil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentinaes_AR
dc.description.filFil: Del Medico Zajac, Maria Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentinaes_AR
dc.description.filFil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad del Salvador; Argentinaes_AR
dc.description.filFil: Blanco Viera, Francisco Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentinaes_AR
dc.description.filFil: Carrillo, Consuelo. USDA. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidoses_AR
dc.description.filFil: Chowdhury, Shafiqul. Louisiana State University. School of Veterinary Medicine. Department of Pathobiological Sciences; Estados Unidoses_AR
dc.description.filFil: Borca, Manuel Victor. USDA. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidoses_AR
dc.description.filFil: Sadir, Ana M. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad del Salvador. Cátedra de Inmunología; Argentinaes_AR
dc.subtypecientifico


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