Mostrar el registro sencillo del ítem

resumen

Resumen
The potato species exhibit gametophytic self-incompatibility system controlled by a single S-locus withmultiple S-haplotypes. Each S-haplotype carries two genetically linked genes, S-RNase and SLF, controllingthe female and male specificities, respectively. In the style, the S-RNase gene codes for an allele-specificribonuclease that is involved in the rejection of pollen that carries the same S-haplotype. This gene hasfive conserved regions (C1–C5) and [ver mas...]
dc.contributor.authorMarcellán, Olga
dc.contributor.authorAcevedo, Alberto
dc.date.accessioned2018-12-10T13:40:23Z
dc.date.available2018-12-10T13:40:23Z
dc.date.issued2014-02
dc.identifier.issn0304-4238
dc.identifier.otherhttps://doi.org/10.1016/j.scienta.2013.12.001
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4038
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0304423813006298?via%3Dihub
dc.description.abstractThe potato species exhibit gametophytic self-incompatibility system controlled by a single S-locus withmultiple S-haplotypes. Each S-haplotype carries two genetically linked genes, S-RNase and SLF, controllingthe female and male specificities, respectively. In the style, the S-RNase gene codes for an allele-specificribonuclease that is involved in the rejection of pollen that carries the same S-haplotype. This gene hasfive conserved regions (C1–C5) and two hypervariable domains located between C2 and C3 that play arole in S-RNase allele specificity. Presently, eight nucleotide sequences of S-RNase alleles from Solanumtuberosum and S. chacoense have been reported in potato species. In an effort to discriminate these S-RNase alleles and eventually detect new allelic variants, these sequences were analyzed by in silico PCR,using primers designed on the basis of four conserved regions, and were further examined by restrictionenzyme patterns. The development of the in silico approach led to the creation of a novel dichotomous keywhich was able to unequivocally identify the eight potato S-RNase alleles already known and eventuallydetect putative alleles when the size and/or the restriction pattern of the amplicons were different fromthose predicted. Experimental data validates the utility and efficiency of the dichotomous keyes_AR
dc.formatapplication/pdfeng
dc.language.isoeng
dc.publisherElsevieres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceScientia horticulturae 166 : 17-23. (13 February 2014)eng
dc.subjectSolanum Tuberosumes_AR
dc.subjectSolanum Chacoensees_AR
dc.subjectPapaes_AR
dc.subjectPotatoeseng
dc.subjectAleloses_AR
dc.subjectAlleleseng
dc.subjectSelf Sterilityeng
dc.subjectAutoesterilidades_AR
dc.subject.otherS-RNase Alleleseng
dc.subject.otherAlelos S-RNasees_AR
dc.subject.otherDichotomous Keyeng
dc.subject.otherClave Dicotómicaes_AR
dc.subject.otherIn Silico Analysiseng
dc.subject.otherAnalisis in Silicoes_AR
dc.subject.otherGametophytic Self-incompatibilityeng
dc.subject.otherAutoincompatibilidad Gametofíticaes_AR
dc.titleA dichotomous key for the discrimination of the already known S-RNase alleles in potato specieseng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Sueloses_AR
dc.description.filFil: Marcellán, Olga. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentinaes_AR
dc.description.filFil: Acevedo, Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; Argentinaes_AR
dc.subtypecientifico


Ficheros en el ítem

Thumbnail

Este ítem aparece en la(s) siguiente(s) colección(ones)

common

Mostrar el registro sencillo del ítem