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Absence of the nitrous oxide reductase gene cluster in commercial alfalfa inoculants is probably due to the extensive loss of genes during rhizobial domestication

Abstract
As other legume crops, alfalfa cultivation increases the emission of the greenhouse gas nitrous oxide (N2O). Since legumesymbiotic nitrogen-fixing bacteria play a crucial role in this emission, it is important to understand the possible impacts of rhizobial domestication on the evolution of denitrification genes. In comparison with the genomes of non-commercial strains, those of commercial alfalfa inoculants exhibit low total genome size, low number of [ver mas...]
As other legume crops, alfalfa cultivation increases the emission of the greenhouse gas nitrous oxide (N2O). Since legumesymbiotic nitrogen-fixing bacteria play a crucial role in this emission, it is important to understand the possible impacts of rhizobial domestication on the evolution of denitrification genes. In comparison with the genomes of non-commercial strains, those of commercial alfalfa inoculants exhibit low total genome size, low number of ORFs and high numbers of both frameshifted genes and pseudogenes, suggesting a dramatic loss of genes during bacterial domestication. Genomic analysis focused on denitrification genes revealed that commercial strains have perfectly conserved the nitrate (NAP), nitrite (NIR) and nitric (NOR) reductase clusters related to the production of N2O from nitrate but completely lost the nitrous oxide (NOS) reductase cluster (nosRZDFYLX genes) associated with the reduction of N2O to gas nitrogen. Based on these results, we propose future screenings for alfalfa-nodulating isolates containing both nitrogen fixation and N2O reductase genes for environmental sustainability of alfalfa production. [Cerrar]
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Author
Brambilla, Silvina Maricel;   Frare, Romina Alejandra;   Soto, Gabriela Cinthia;   Jozefkowicz, Cintia;   Ayub, Nicolás Daniel;  
Fuente
Microbial Ecology 76 (2): 299–302 (August 2018)
Date
2018-08
Editorial
Springer
ISSN
0095-3628
1432-184X
URI
https://link.springer.com/article/10.1007/s00248-018-1145-9
http://hdl.handle.net/20.500.12123/3399
DOI
https://doi.org/10.1007/s00248-018-1145-9
Formato
pdf
Tipo de documento
artículo
Palabras Claves
Óxido Nitroso; Medicago sativa; Fijación del Nitrógeno; Muestreo cluster; Genes; Rhizobium; Nitrous Oxide; Nitrogen Fixation; Cluster Sampling; Inoculantes comerciales;
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