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Abstract
As other legume crops, alfalfa cultivation increases the emission of the greenhouse gas nitrous oxide (N2O). Since legumesymbiotic nitrogen-fixing bacteria play a crucial role in this emission, it is important to understand the possible impacts of rhizobial domestication on the evolution of denitrification genes. In comparison with the genomes of non-commercial strains, those of commercial alfalfa inoculants exhibit low total genome size, low number of [ver mas...]
dc.contributor.authorBrambilla, Silvina Maricel
dc.contributor.authorFrare, Romina Alejandra
dc.contributor.authorSoto, Gabriela Cinthia
dc.contributor.authorJozefkowicz, Cintia
dc.contributor.authorAyub, Nicolás Daniel
dc.date.accessioned2018-09-19T17:04:53Z
dc.date.available2018-09-19T17:04:53Z
dc.date.issued2018-08
dc.identifier.issn0095-3628
dc.identifier.issn1432-184X
dc.identifier.otherhttps://doi.org/10.1007/s00248-018-1145-9
dc.identifier.urihttps://link.springer.com/article/10.1007/s00248-018-1145-9
dc.identifier.urihttp://hdl.handle.net/20.500.12123/3399
dc.description.abstractAs other legume crops, alfalfa cultivation increases the emission of the greenhouse gas nitrous oxide (N2O). Since legumesymbiotic nitrogen-fixing bacteria play a crucial role in this emission, it is important to understand the possible impacts of rhizobial domestication on the evolution of denitrification genes. In comparison with the genomes of non-commercial strains, those of commercial alfalfa inoculants exhibit low total genome size, low number of ORFs and high numbers of both frameshifted genes and pseudogenes, suggesting a dramatic loss of genes during bacterial domestication. Genomic analysis focused on denitrification genes revealed that commercial strains have perfectly conserved the nitrate (NAP), nitrite (NIR) and nitric (NOR) reductase clusters related to the production of N2O from nitrate but completely lost the nitrous oxide (NOS) reductase cluster (nosRZDFYLX genes) associated with the reduction of N2O to gas nitrogen. Based on these results, we propose future screenings for alfalfa-nodulating isolates containing both nitrogen fixation and N2O reductase genes for environmental sustainability of alfalfa production.es_AR
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherSpringeres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceMicrobial Ecology 76 (2): 299–302 (August 2018)es_AR
dc.subjectÓxido Nitrosoes_AR
dc.subjectMedicago sativaes_AR
dc.subjectFijación del Nitrógenoes_AR
dc.subjectMuestreo clusteres_AR
dc.subjectGeneses_AR
dc.subjectRhizobiumes_AR
dc.subjectNitrous Oxideeng
dc.subjectNitrogen Fixationeng
dc.subjectCluster Samplingeng
dc.subject.otherInoculantes comercialeses_AR
dc.titleAbsence of the nitrous oxide reductase gene cluster in commercial alfalfa inoculants is probably due to the extensive loss of genes during rhizobial domesticationes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Genéticaes_AR
dc.description.filFil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentinaes_AR
dc.description.filFil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Jozefkowicz, Cintia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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