Listar Artículos científicos por fecha de publicación
Mostrando ítems 1-20 de 296
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Use of touch-down polymerase chain reaction to enhance the sensitivity of Mycobacterium bovis detection
(Sage Publications, 2005-05)The confirmatory diagnosis of Mycobacterium bovis (M. bovis) in animal samples is carried out by culture in Stonebrink media. However, culture is very slow because of the extremely long duplication time of the bacillus and ... -
Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants
(Wiley-Blackwell, 2008-05)Snakin‐1 (SN1), a cysteine‐rich peptide with broad‐spectrum antimicrobial activity in vitro, was evaluated for its ability to confer resistance to pathogens in transgenic potatoes. Genetic variants of this gene were cloned ... -
A candidate gene survey of quantitative trait loci affecting chemical composition in tomato fruit
(Society for Experimental Biology, 2008-07)In tomato, numerous wild-related species have been demonstrated to be untapped sources of valuable genetic variability, including pathogen-resistance genes, nutritional, and industrial quality traits. From a collection of ... -
Virus infection elevates transcriptional activity of miR164a promoter in plants
(BioMed Central, 2009-12)Background: Micro RNAs (miRs) constitute a large group of endogenous small RNAs that have crucial roles in many important plant functions. Virus infection and transgenic expression of viral proteins alter accumulation and ... -
Developments in plant-based vaccines against diseases of concern in developing countries
(BENTHAM Open, 2010)Vaccination has been extensively used to prevent, eradicate and control infectious diseases. However, the development and implementation of new vaccines remains prohibitively expensive for middle and low income countries where ... -
Differences in the virulence of two strains of Foot-and-Mouth Disease Virus Serotype A with the same spatiotemporal distribution
(Elsevier, 2010-01)During the 2000–2001 epidemic of Foot-and-Mouth Disease Virus (FMDV) in Argentina, two FMDV serotype A viruses were identified among others. Since different pathogenic properties between these virus strains were noticed ... -
Trans-complementation of polyhedrin by a stably transformed Sf9 insect cell line allows occ− baculovirus occlusion and larval per os infectivity
(Elsevier, 2010-01-15)Oral infection of insect larvae with baculovirus is an advantageous methodology for producing high levels of recombinant proteins and for achieving plague control. However, many recombinant baculoviruses express a foreign ... -
Preparation of a working seed lot of BCG and quality control by PCR genotyping
(Asociación Argentina de Microbiología, 2010-04)The bacillus Calmette-Guérin (BCG) was obtained in 1920 after successive passages leading to the attenuation of a Mycobacterium bovis strain. For the following 40 years, BCG had been replicated, resulting in substrains ... -
Identification of four novel DC-SIGN ligands on Mycobacterium bovis BCG
(Springer, 2010-09)Dendritic-cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN; CD209) has an important role in mediating adherence of Mycobacteria species, including M. tuberculosis and M. bovis BCG to human ... -
Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding
(Pontificia Universidad Catolica de Valparaiso, 2010-09)Snakin-1 (StSN1) is a broad-spectrum antimicrobial peptide isolated from Solanum tuberosum. Homologous proteins have been identified in a wide range of species but there is no apparent consensus in the roles they play. A ... -
Coupling virus-induced gene silencing to exogenous green fluorescence protein expression provides a highly efficient system for functional genomics in arabidopsis and across all stages of tomato fruit development
(2011-07)Since the advent of the postgenomic era, efforts have focused on the development of rapid strategies for annotating plant genes of unknown function. Given its simplicity and rapidity, virus-induced gene silencing (VIGS) ... -
Genetic dissection of vitamin E biosynthesis in tomato
(Society for Experimental Biology, 2011-07)Vegetables are critical for human health as they are a source of multiple vitamins including vitamin E (VTE). In plants, the synthesis of VTE compounds, tocopherol and tocotrienol, derives from precursors of the shikimate ... -
Respuesta inmune en terneros experimentalmente infectados con diferentes dosis de Mycobacterium bovis
(Gerencia de Comunicación e Imagen Institucional, DNA SICC, INTA, 2011-08)La tuberculosis bovina es una enfermedad zoonótica producida por Mycobacterium bovis que afecta a animales de producción causando pérdidas económicas y representa, además, un problema para la salud pública. El conocimiento ... -
Metabolic and miRNA profiling of TMV infected plants reveals biphasic temporal changes
(2011-12-12)Plant viral infections induce changes including gene expression and metabolic components. Identification of metabolites and microRNAs (miRNAs) differing in abundance along infection may provide a broad view of the pathways ... -
Transgenic expression of tobacco mosaic virus capsid and movement proteins modulate plant basal defense and biotic stress responses in Nicotiana tabacum
(2012)Plant viruses cause metabolic and physiological changes associated with symptomatic disease phenotypes. Symptoms involve direct and indirect effects, which result in disruption of host physiology. We used transgenic ... -
Snakin/GASA proteins : involvement in hormone crosstalk and redox homeostasis
(Taylor & Francis, 2012)Snakin/GASA proteins are widely distributed among plant species. They are expressed in different plant organs with high tissue and temporal specificity, and their subcellular localization varies among the different members. ... -
Potato Snakin-1 Gene Silencing Affects Cell Division, Primary Metabolism, and Cell Wall Composition
(American Society of Plant Biologists, 2012-01)Snakin-1 (SN1) is an antimicrobial cysteine-rich peptide isolated from potato (Solanum tuberosum) that was classified as a member of the Snakin/Gibberellic Acid Stimulated in Arabidopsis protein family. In this work, a ... -
Surface Display of AcMNPV Occlusion-Derived P74 Does Not Enhance Oral Infectivity of Budded Viruses
(S. Karger AG, 2012-02)Baculovirus occlusion-derived viruses (ODVs) and budded viruses (BVs) are morphologically and functionally distinct. ODVs are responsible for primary infection in insect hosts because of their high per os infectivity. On ... -
Detection of Mycobacterium bovis–infected dairy herds using PCR in bulk tank milk samples
(Mary Ann Liebert Inc., 2012-02)Bovine tuberculosis (bTB) is a chronic and zoonotic disease due to Mycobacterium bovis. The tuberculosis eradication campaign carried out in Argentina has considerably improved the health situation of the herds. Here we ... -
Genetic diversity of maize landraces from lowland and highland agro‐ecosystems of Southern South America: implications for the conservation of native resources
(Wiley, 2012-05)The North of Argentina is one of the southernmost areas of maize landrace cultivation. Two distinct centres of diversity have been distinguished within this region: Northwestern Argentina (NWA), and Northeastern Argentina ...