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Mostrando ítems 1-17 de 17

    • A fijivirus major viroplasm protein shows RNA-stimulated ATPase activity by adopting pentameric and hexameric assemblies of dimers 

      Llauger, Gabriela; Melero, Roberto; Monti, Demian Esteban; Sycz, Gabriela; Huck-Iriart, Cristian; Cerutti, Maria Laura; Klinke, Sebastián; Mikkelsen, Evelyn; Tijman, Ariel; Arranz, Rocío; Alfonso, Victoria; Arellano, Sofía Maité; Goldbaum, Fernando Alberto; Sterckx, Yann G. J.; Carazo, José María; Kaufman, Sergio B.; Dans, Pablo D.; Del Vas, Mariana; Otero, Lisandro H. (American Society for Microbiology, 2023-02)
      Fijiviruses replicate and package their genomes within viroplasms in a pro cess involving RNA-RNA and RNA-protein interactions. Here, we demonstrate that the 24 C-terminal residues (C-arm) of the P9-1 major viroplasm protein ...

    • AcMNPV Core Gene ac109 Is Required for Budded Virion Transport to the Nucleus and for Occlusion of Viral Progeny 

      Alfonso, Victoria; Maroniche, Guillermo Andrés; Reca, Sol Rita; Lopez, Maria Gabriela; Del Vas, Mariana; Taboga, Oscar Alberto (Plos One, 2012-09)
      The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac109 core gene has been previously characterized as an essential late gene. Our results showed that budded virions could be detected in supernatants of ...

    • Development of a novel set of Gateway-compatible vectors for live imaging in insect cells 

      Maroniche, Guillermo Andrés; Mongelli, Vanesa Claudia; Alfonso, Victoria; Llauger, Gabriela; Taboga, Oscar Alberto; Del Vas, Mariana (The Royal Entomological Society, 2011-10)
      Insect genomics is a growing area of research. To exploit fully the genomic data that are being generated, high-throughput systems for the functional characterization of insect proteins and their interactomes are required. ...

    • Development of nanobodies against Mal de Río Cuarto virus major viroplasm protein P9‑1 for diagnostic sandwich ELISA and immunodetection 

      Llauger, Gabriela; Monti, Demian Esteban; Aduriz Guerrero, Matías; Romão, Ema; Dumon, Analia Delina; Mattio, Maria Fernanda; Wigdorovitz, Andres; Muyldermans, Serge; Vincke, Cécile; Parreño, Viviana; Del Vas, Mariana (Springer Nature, 2021-10)
      Mal de Río Cuarto virus (MRCV) is a member of the genus Fijivirus of the family Reoviridae that causes a devastating disease in maize and is persistently and propagatively transmitted by planthopper vectors. Virus replication ...

    • Functional and biochemical properties of Mal de Río Cuarto virus (Fijivirus, Reoviridae) P9-1 viroplasm protein show further similarities to animal reovirus counterparts 

      Maroniche, Guillermo Andrés; Mongelli, Vanesa Claudia; Peralta, Andrea Veronica; Distefano, Ana Julia; Llauger, Gabriela; Taboga, Oscar Alberto; Hopp, Horacio Esteban; Del Vas, Mariana (Elsevier, 2010-09)
      Mal de Río Cuarto virus (MRCV) is a plant virus of the genus Fijivirus within the family Reoviridae that infects several monocotyledonous species and is transmitted by planthoppers in a persistent and propagative manner. ...

    • Functional characterization and intracellular localization of a Lotus tenuis NHX antiporter 

      Affinito, María Agostina; Espasandin, Fabiana Daniela; Del Vas, Mariana; Alvarez, Mayra Yanet; Andres, Adriana Noemi; Sansberro, Pedro Alfonso; Diaz Paleo, Antonio Horacio (Springer, 2021-05)
      Lotus tenuis is a perennial forage legume widely scattered in the flooding Pampa region of Argentina. Even when it is considered a salt-tolerant species, the molecular basis underlying this trait still remains unknown. ...

    • Genome-wide identification of MITE-derived microRNAs and their targets in bread wheat 

      Crescente, Juan Manuel; Zavallo, Diego; Del Vas, Mariana; Asurmendi, Sebastian; Helguera, Marcelo; Fernandez, Elmer; Vanzetti, Leonardo Sebastian (BioMed Central, 2022-02)
      Background: Plant miRNAs are a class of small non-coding RNAs that can repress gene expression at the post-transcriptional level by targeting RNA degradation or promoting translational repression. There is increasing ...

    • ICTV virus taxonomy profile : Sedoreoviridae 2022 

      Matthijnssens, Jelle; Attoui, Houssam; Bányai, Krisztián; Brussaard, Corina P.D.; Danthi, Pranav; Del Vas, Mariana; Dermody, Terence S.; Duncan, Roy; Fāng, Qín; Johne, Reimar; Mertens, Peter P.C.; Jaafar, Fauziah Mohd; Patton, John T.; Sasaya, Takahide; Suzuki, Nobuhiro; Wei, Taiyun (Microbiology Society, 2022-10)
      Sedoreoviridae is a large family of icosahedral viruses that are usually regarded as non- enveloped with segmented (10–12 linear segments) dsRNA genomes of 18–26 kbp. Sedoreovirids have a broad host range, infecting mammals, ...

    • In vivo subcellular localization of Mal de Río Cuarto virus (MRCV) non-structural proteins in insect cells reveals their putative functions 

      Maroniche, Guillermo Andrés; Mongelli, Vanesa Claudia; Llauger, Gabriela; Alfonso, Victoria; Taboga, Oscar Alberto; Del Vas, Mariana (Elsevier, 2012-09)
      The in vivo subcellular localization of Mal de Río Cuarto virus (MRCV, Fijivirus, Reoviridae) non-structural proteins fused to GFP was analyzed by confocal microscopy. P5-1 showed a cytoplasmic vesicular-like distribution ...

    • Interaction of Mal de Río Cuarto virus (Fijivirus genus) proteins and identification of putative factors determining viroplasm formation and decay 

      Llauger, Gabriela; De Haro, Luis Alejandro; Alfonso, Victoria; Del Vas, Mariana (2017)
      Mal de Río Cuarto virus (MRCV) is a member of the Fijivirus genus, within the Reoviridae family, that repli-cates and assembles in cytoplasmic inclusion bodies called viroplasms. In this study, we investigatedinteractions ...

    • Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein 

      Distefano, Ana Julia; Maldonado, Sara Beatriz; Hopp, Horacio Esteban; Del Vas, Mariana (Springer, 2009-06)
      Mal de Río Cuarto virus (MRCV) is a newly described species of the genus Fijivirus, family Reoviridae. Compared with other plant-infecting genus of the family, the function and localization of MRCV and other Fijivirus ...

    • Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves 

      De Haro, Luis Alejandro; Arellano, Sofía Maité; Novak, Ondrej; Feil, Regina; Dumon, Analia Delfina; Mattio, Maria Fernanda; Tarkowska, Danuse; Llauger, Gabriela; Strnad, Miroslav; Lunn, John Edward; Pearce, Stephen; Figueroa, Carlos María; Del Vas, Mariana (BMC, 2019-03)
      Background; Mal de Río Cuarto virus (MRCV) infects several monocotyledonous species including maize and wheat. Infected plants show shortened internodes, partial sterility, increased tillering and reduced root length. To ...

    • Mal de Río Cuarto Virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and Its planthopper vector 

      De Haro, Luis Alejandro; Dumon, Analia Delina; Mattio, Maria Fernanda; Arguello Caro, Evangelina Beatriz; Llauger, Gabriela; Zavallo, Diego; Blanc, Hervé; Mongelli, Vanesa Claudia; Truol, Graciela Ana Maria; Saleh, María-Carla; Asurmendi, Sebastian; Del Vas, Mariana (2017)
      Plant reoviruses are able to multiply in gramineae plants and delphacid vectors encountering different defense strategies with unique features. This study aims to comparatively assess alterations of small RNA (sRNA) ...

    • Negative modulation of the GABAAρ1 receptor function by histamine 

      Beltrán González, Andrea N.; López Pazos, Manuel I.; Del Vas, Mariana; Calvo, Daniel Juan (Elsevier, 2023-09)
      Besides its function as a local mediator of the immune response, histamine can play a role as a neurotransmitter and neuromodulator. Histamine actions are classically mediated through four different G protein-coupled ...

    • Reference gene selection for gene expression studies using RT-qPCR in virus-infected planthoppers 

      Maroniche, Guillermo Andrés; Sagadin, Monica Beatriz; Mongelli, Vanesa Claudia; Truol, Graciela Ana Maria; Del Vas, Mariana (BioMed Central, 2011-12)
      Background: Planthoppers not only severely affect crops by causing mechanical damage when feeding but are also vectors of several plant virus species. The analysis of gene expression in persistently infected planthoppers ...

    • Sequence analysis of genome segments S5 and S10 of Mal de Río Cuarto virus (Fijivirus, Reoviridae) 

      Distefano, Ana Julia; Hopp, Horacio Esteban; Del Vas, Mariana (Springer, 2005-06)
      Mal de Río Cuarto virus (MRCV) was recently described as a new species of the genus Fijivirus, family Reoviridae. The nucleotide sequence of two MRCV genome segments was determined. MRCV S5 and S10 were predicted to encode ...

    • Virus infection elevates transcriptional activity of miR164a promoter in plants 

      Bazzini, Ariel Alejandro; Almasia, Natalia Ines; Manacorda, Carlos Augusto; Mongelli, Vanesa Claudia; Conti, Gabriela; Maroniche, Guillermo Andrés; Rodriguez, Maria Cecilia; Distefano, Ana Julia; Hopp, Horacio Esteban; Del Vas, Mariana; Asurmendi, Sebastian (BioMed Central, 2009-12)
      Background: Micro RNAs (miRs) constitute a large group of endogenous small RNAs that have crucial roles in many important plant functions. Virus infection and transgenic expression of viral proteins alter accumulation and ...