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Mostrando ítems 1-7 de 7
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AcMNPV Core Gene ac109 Is Required for Budded Virion Transport to the Nucleus and for Occlusion of Viral Progeny
Alfonso, Victoria; Maroniche, Guillermo Andrés; Reca, Sol Rita; Lopez, Maria Gabriela; Del Vas, Mariana; Taboga, Oscar Alberto (Plos One, 2012-09)The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac109 core gene has been previously characterized as an essential late gene. Our results showed that budded virions could be detected in supernatants of ... -
Development of a novel set of Gateway-compatible vectors for live imaging in insect cells
Maroniche, Guillermo Andrés; Mongelli, Vanesa Claudia; Alfonso, Victoria; Llauger, Gabriela; Taboga, Oscar Alberto; Del Vas, Mariana (The Royal Entomological Society, 2011-10)Insect genomics is a growing area of research. To exploit fully the genomic data that are being generated, high-throughput systems for the functional characterization of insect proteins and their interactomes are required. ... -
Functional and biochemical properties of Mal de Río Cuarto virus (Fijivirus, Reoviridae) P9-1 viroplasm protein show further similarities to animal reovirus counterparts
Maroniche, Guillermo Andrés; Mongelli, Vanesa Claudia; Peralta, Andrea Veronica; Distefano, Ana Julia; Llauger, Gabriela; Taboga, Oscar Alberto; Hopp, Horacio Esteban; Del Vas, Mariana (Elsevier, 2010-09)Mal de Río Cuarto virus (MRCV) is a plant virus of the genus Fijivirus within the family Reoviridae that infects several monocotyledonous species and is transmitted by planthoppers in a persistent and propagative manner. ... -
In vivo subcellular localization of Mal de Río Cuarto virus (MRCV) non-structural proteins in insect cells reveals their putative functions
Maroniche, Guillermo Andrés; Mongelli, Vanesa Claudia; Llauger, Gabriela; Alfonso, Victoria; Taboga, Oscar Alberto; Del Vas, Mariana (Elsevier, 2012-09)The in vivo subcellular localization of Mal de Río Cuarto virus (MRCV, Fijivirus, Reoviridae) non-structural proteins fused to GFP was analyzed by confocal microscopy. P5-1 showed a cytoplasmic vesicular-like distribution ... -
Reference gene selection for gene expression studies using RT-qPCR in virus-infected planthoppers
Maroniche, Guillermo Andrés; Sagadin, Monica Beatriz; Mongelli, Vanesa Claudia; Truol, Graciela Ana Maria; Del Vas, Mariana (BioMed Central, 2011-12)Background: Planthoppers not only severely affect crops by causing mechanical damage when feeding but are also vectors of several plant virus species. The analysis of gene expression in persistently infected planthoppers ... -
Successful production of the potato antimicrobial peptide Snakin-1 in baculovirus-infected insect cells and development of specific antibodies
Almasia, Natalia Ines; Molinari, Maria Paula; Maroniche, Guillermo Andrés; Nahirñak, Vanesa; Barrios Baron, Maria Pilar; Taboga, Oscar Alberto; Vazquez Rovere, Cecilia (2017)Background: Snakin-1 (StSN1) is a broad-spectrum antimicrobial cysteine-rich peptide isolated from Solanum tuberosum. Its biotechnological potential has been already recognized since it exhibits in vivo antifungal and ... -
Virus infection elevates transcriptional activity of miR164a promoter in plants
Bazzini, Ariel Alejandro; Almasia, Natalia Ines; Manacorda, Carlos Augusto; Mongelli, Vanesa Claudia; Conti, Gabriela; Maroniche, Guillermo Andrés; Rodriguez, Maria Cecilia; Distefano, Ana Julia; Hopp, Horacio Esteban; Del Vas, Mariana; Asurmendi, Sebastian (BioMed Central, 2009-12)Background: Micro RNAs (miRs) constitute a large group of endogenous small RNAs that have crucial roles in many important plant functions. Virus infection and transgenic expression of viral proteins alter accumulation and ...