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Abstract
The genus Glandularia (Verbenaceae) has more than 50 species and it holds great ornamental potential due to its colorful flowers, long flowering period and low water requirements. Since the floriculture market avidly seeks novelties, increasing the diversity within the genus would increase its commercial value. Some of the traits that should be improved in these species are their architecture and the size and colors of the flowers and leaves. In this [ver mas...]
dc.contributor.authorGonzalez Roca, Lucia
dc.contributor.authorIannicelli, Jesica
dc.contributor.authorCoviella, Maria Andrea
dc.contributor.authorBugallo, Veronica Lucia
dc.contributor.authorBologna, Paula
dc.contributor.authorPitta Alvarez, Sandra Irene
dc.contributor.authorEscandon, Alejandro Salvio
dc.date.accessioned2018-07-26T12:55:08Z
dc.date.available2018-07-26T12:55:08Z
dc.date.issued2015-03-05
dc.identifier.issn0304-4238
dc.identifier.otherhttps://doi.org/10.1016/j.scienta.2014.12.032
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0304423814007055
dc.identifier.urihttp://hdl.handle.net/20.500.12123/2883
dc.description.abstractThe genus Glandularia (Verbenaceae) has more than 50 species and it holds great ornamental potential due to its colorful flowers, long flowering period and low water requirements. Since the floriculture market avidly seeks novelties, increasing the diversity within the genus would increase its commercial value. Some of the traits that should be improved in these species are their architecture and the size and colors of the flowers and leaves. In this sense, obtaining polyploid individuals is an interesting strategy to achieve this objective. In this paper a hybrid of Glandularia peruviana × Glandularia scrobiculata was cultured under in vitro conditions. Different plant growth regulators and their combinations were tested to obtain appropriate multiplication rates. The best results were obtained with the combination of 6.6 μM thidiazuron/0.03 μM α-naphthalenacetic acid, with a multiplication rate of 19 shoots per explant. The plantlets were first rooted and then acclimatized by transferal to an 8.0 cm diameter pot containing Growing mix®, and maintenance inside a humidity chamber. For poliploydization, the explants were exposed to colchicine in concentrations of 0.001% and 0.01% for 24 and 48 h. The 40 recovered plants were characterized according to their DNA content. There were 21 diploids, 14 solid tetraploids, 1 solid octoploid, 3 chimera tetraploids and 1 chimera octoploid. Phenotypically, the size of the flowers, inflorescences, pollen grains and stomata were significantly larger in polyploid individuals. Surprisingly, stem diameter, leaf size and color intensity of the leaves and flowers were not significantly different between diploid and tetraploid individuals. Due to the size of the inflorescences, the tetraploid individuals are a promising starting material for a Glandularia breeding program.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceScientia Horticulturae 184 : 46-54 (March 2015)es_AR
dc.subjectVerbenaceaees_AR
dc.subjectExperimentación in Vitroes_AR
dc.subjectIn Vitro Experimentationeng
dc.subjectPoliploidia Inducidaes_AR
dc.subjectInduced Polyploidyeng
dc.subjectPropagación Vegetativaes_AR
dc.subjectVegetative Propagationeng
dc.subjectHibridación Interespecíficaes_AR
dc.subjectInterspecific Hybridizationeng
dc.subject.otherGlandulariaes_AR
dc.titleA protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana × G. scrobiculata)es_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Floriculturaes_AR
dc.description.filFil: Gonzalez Roca, Lucia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentinaes_AR
dc.description.filFil: Iannicelli, Jesica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentinaes_AR
dc.description.filFil: Coviella, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentinaes_AR
dc.description.filFil: Bugallo, Verónica. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Genética; Argentinaes_AR
dc.description.filFil: Bologna, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentinaes_AR
dc.description.filFil: Pitta Alvarez, Sandra Irene. Universidad Argentina de la Empresa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Biotecnología Algal y Vegetal; Argentinaes_AR
dc.description.filFil: Escandon, Alejandro Salvio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentinaes_AR
dc.subtypecientifico


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